Abstract
Metagenomics, shotgun metagenomics in particular, is a field that has disproportionately benefited from advances in high-throughput sequencing technologies. This chapter discusses the choice of sample number, depth, and sequencing technology, the shearing, size-selection, and multiplexing options on Illumina platforms, and outlines the steps in the bioinformatic analysis and sharing of metagenomic data.
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Annex: Quick Reference Guide
Annex: Quick Reference Guide
Representation of the wet-lab procedure workflow
Main steps of the computational analysis pipeline
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Trimble, W.L., Greenwald, S.M., Owens, S., Glass, E.M., Meyer, F. (2016). Metagenomic Design and Sequencing. In: Aransay, A., LavÃn Trueba, J. (eds) Field Guidelines for Genetic Experimental Designs in High-Throughput Sequencing. Springer, Cham. https://doi.org/10.1007/978-3-319-31350-4_12
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DOI: https://doi.org/10.1007/978-3-319-31350-4_12
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