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Linkage Mapping and QTL Analysis

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The Jatropha Genome

Part of the book series: Compendium of Plant Genomes ((CPG))

Abstract

Jatropha curcas L. is a potential plant species for biodiesel production. However, its seed yield is too low for profitable production of biodiesel. To improve the productivity, genetic improvement through breeding is essential. Marker-assisted selection (MAS) has the huge potential to accelerate genetic improvement. We mapped 506 markers (216 microsatellites and 290 SNPs from ESTs) onto 11 linkage groups. However, genetic analysis of the yield traits has not been done in jatropha. Quantitative trait loci (QTL) mapping was conducted to identify genetic factors controlling growth and seed yield in jatropha. We identified a total of 28 QTLs for 11 growth and seed traits using a population of 296 backcrossing jatropha trees. QTL and expression QTL analyses were applied to identify genetic factors that are relevant to seed oil traits in jatropha. We screened key genes in auxin pathway including ARF and IAA families and downstream effectors to identify candidate genes controlling seed size in jatropha. JcARF19 was mapped in the major QTL region and significantly associated with seed length.

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Acknowledgements

The work is part of the project “Genetic Improvement of Jatropha” initiated and coordinated by Professor Nam-Hai Chua, Rockefeller University, USA. We thank Weijie Tang, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, 210095, Nanjing, China, for his editing work on this chapter. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of this chapter.

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Authors and Affiliations

  1. Temasek Life Sciences Laboratory, National, University of Singapore, 1 Research Link, 117604, Singapore, Singapore

    Jian Ye, Chunming Wang & Genhua Yue

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  1. Jian Ye
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  2. Chunming Wang
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  3. Genhua Yue
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Correspondence to Chunming Wang or Genhua Yue .

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Editors and Affiliations

  1. Graduate School of Engineering, Osaka University, Suita, Osaka, Japan

    Suguru Tsuchimoto

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Ye, J., Wang, C., Yue, G. (2017). Linkage Mapping and QTL Analysis. In: Tsuchimoto, S. (eds) The Jatropha Genome. Compendium of Plant Genomes. Springer, Cham. https://doi.org/10.1007/978-3-319-49653-5_2

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