Abstract
To understand the effect of peptone on the formation of shoot apical meristem during early development of orchid embryo, the cytological characterization of orchid embryo developmental phases was conducted. Three- to four-month-old fruits and seeds were sown on Vacin and Went (VW) medium for Dendrobium phalaenopsis and Dendrobium lineale and New Phalaenopsis (NP) medium for Phalaenopsis amabilis. The medium were added with 1 g · L−1 to 3 g · L−1 peptone. Cultures were maintained at 25 °C with continuous white light. Anatomic preparations were done using paraffin methods and observed every week. The result showed that orchid embryo germinated and formed shoot through five phases, namely, phase 0 (early embryo), phase 1 (swollen embryo, enlarged cell size), phase 2 (ovoid-shaped embryo, cell shape becomes ovoid and testa was broken), phase 3 (no testa, formed bipolar apical and basal cells), phase 4 (embryo forms tuber-like shape with absorbing hair in the basal part, termed protocorm) and phase 5 (protocorm formed shoot with dense colour cells in the shoot apical meristem, termed SAM). SAM was initiated at 3 weeks after seed sowing (WAS). The best concentration of additional peptone in the medium to accelerate the development of orchid embryos was 2 g · L−1 for Dendrobium and 3 g · L−1 for Phalaenopsis, which start from very early stages (phase 1 or phase 2).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Arditti, J.: Factors affecting the germination of orchid seeds. Bot. Rev. 33(1), 1–97 (1967)
Shekarriz, P., et al.: Coconut water and peptone improve seed germination and protocorm like body formation of hybrid Phalaenopsis. Agric. Sci. Dev. 3(10), 317–322 (2014)
Semiarti, E., et al.: Agrobacterium-mediated transformation of Indonesian orchid for micropropagation. http://cdn.intechopen.com/pdfs/18821/InTechAgrobacterium_mediated_transformation_of_indonesian_orchids_for_micropropagation.pdf (2011). Accessed 12 April 2016
George, E.F., Sherrington, P.D.: Plant Propagation by Tissue Culture. Exegetics Limited, Basingstoke (1984)
Da Silva, J.A.T.: Ammonium to nitrate ratio affects protocorm like bodies PLB formation in vitro of hybrid Cymbidium. J. Ornam. Hortic. Plant. 3(3), 155–160 (2013)
Taylor, et al.: Biological efficacy of several commercially available peptones for mammalian cells in culture. Exp. Cell Res. 74, 275–279 (1972)
Sakoda, F.: Establishment and characterization of a porcine kidney cell line, FS-L3, which forms unique multicellular domes in serum-free culture. In Vitro Cell. Dev. Biol. An. 34(53–57), (1998)
Semiarti, E., et al.: High-frequency genetic transformation of Phalaenopsis amabilis orchid using tomato extract-enriched medium for the pre-culture of protocorms. J. Hortic. Sci. Biotechnol. 85(3), 205–210 (2010)
David, et al.: Organic additives improves the in vitro growth of native orchid Vanda helvola Blume. Not. Sci. Biol. 7(2), 192–197 (2015)
Roy, J., Banerjee, N.: Cultural requirement for in vitro seed germination, protocorm growth and seedling development of Geodorum densiflorum (Lam.) Schltr. Indian J. Exp. Biol. 39, 1041–1047 (2001)
Kauth, P. J., et al.: Seed culture and seedling development of Calopogon tuberosus. Plant Cell Tiss. Org. Cult. In Vitro. 85, 91–102 (2006)
Indrianto, A.: Kultur Jaringan Tumbuhan. [Plant Tissue Culture]. Faculty of Biology UGM, Yogyakarta (2003) (in Bahasa Indonesia)
Davami, F., et al.: Effects of peptone supplementation in different culture media on growth, metabolic pathway and productivity of CHO DG44 cells; a new insight into amino acid profiles. Iran. Biomed. J. 19(4), 194–205 (2015)
Mondal, S., et al.: Effect of coconut water and ascorbic acid on shoot regeneration in banana variety dwarf Cavendish. Int. J. Bio. Res. Environ. Agric. Sci. 1(1), 65–69 (2015)
Hossain, M.M.: Asymbiotic seed germination and in vitro seedling development of Epidendrum ibaguense Kunth. (Orchidaceae). Afr. J. Biotechnol. 7(20), 3614–3619 (2008)
Soad, et al.: Influence of foliar application of peptone on growth, flowering and chemical composition of Helichrysum bracteatum plants under different irrigation intervals. Ozean J. Appl. Sci. 3(1), 143–155 (2010)
Acknowledgements
This work was supported by PUPT UGM grant from the Ministry of Research and Higher Education, Republic of Indonesia, No. 661/UN1-P.III/LT/DIT-LIT/2016, and in part it was supported by Research Grant BPPTNBh 2016 Faculty of Biology UGM No. UGM/BI/1945/UM/01/39.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer International Publishing AG
About this paper
Cite this paper
Semiarti, E., Setiari, N., Astutiningrum, W.D., Nurliana, S., Mose, W. (2017). The Effect of Peptone on Embryo Development of Orchid During In Vitro Culture. In: Isnansetyo, A., Nuringtyas, T. (eds) Proceeding of the 1st International Conference on Tropical Agriculture. Springer, Cham. https://doi.org/10.1007/978-3-319-60363-6_8
Download citation
DOI: https://doi.org/10.1007/978-3-319-60363-6_8
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-60362-9
Online ISBN: 978-3-319-60363-6
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)