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Probing Membrane Heterogeneity with k-space Image Correlation Spectroscopy

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Membrane Organization and Dynamics

Part of the book series: Springer Series in Biophysics ((BIOPHYSICS,volume 20))

Abstract

Understanding and quantifying the plasma membrane complexity, with its myriad of structures and intricate interactions within and with components inside and outside of the cell boundary is one of paramount importance in the field of medical sciences. Indeed, when it comes to design and development of novel drug therapies targeting of G-protein coupled receptors (GPCR) and similar protein complexes, seeing the interaction of these proteins in situ with major players involved in drug uptake requires novel tools that can follow these process in live cells by fluorescence microscopy. In past 10 years, k-space Image Correlation Spectroscopy (kICS) was demonstrated to be a simple to implement and reliable approach for such studies. In this chapter we introduce the kICS methodology, followed by series of examples of its adaptations used in different case studies of membrane protein dynamics and kinetics in live cell membrane environment.

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Author information

Authors and Affiliations

  1. Biomedical Imaging Facility—Mark Wainwright Analytical Centre, University of New South Wales, Sydney, Australia

    Elvis Pandzic

  2. Departments of Physics and Chemistry, McGill University, Montreal, Canada

    Paul W. Wiseman

Authors
  1. Elvis Pandzic
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  2. Paul W. Wiseman
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Corresponding author

Correspondence to Paul W. Wiseman .

Editor information

Editors and Affiliations

  1. Centre for Cellular & Molecular Biology , Hyderabad, India

    Amitabha Chattopadhyay

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Pandzic, E., Wiseman, P.W. (2017). Probing Membrane Heterogeneity with k-space Image Correlation Spectroscopy. In: Chattopadhyay, A. (eds) Membrane Organization and Dynamics . Springer Series in Biophysics, vol 20. Springer, Cham. https://doi.org/10.1007/978-3-319-66601-3_7

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