Skip to main content
SpringerLink
Log in

Biotechnological Approaches for In Vitro Conservation of Picrorhiza kurroa Royle ex Benth

  • Chapter
Medicinal Plants and Environmental Challenges

  • 1321 Accesses

Abstract

Medicinal plants have been used by humans for the treatment of various diseases since ancient times. The plants being rich in secondary metabolites are exploited for their probable utility due to the presence of phytochemical properties. The pure compound can easily be obtained from the plants resulting in safer drugs. One of such important medicinal plant is Picrorhiza kurroa which is a principle source of iridoid glycosides, with a wide range of biological activities. Today the plant has become endangered due to restricted distribution and unskilled harvesting and is demanding to be conserved for its posterity. In order to safeguard and preserve such medicinally important plant species, certain biotechnological approaches are found to be very valuable for their conservation and further manipulation so as to augment their yield and quality traits.

The original version of this chapter was revised: New chapter has been included at the end. The erratum to this chapter is available at https://doi.org/10.1007/978-3-319-68717-9_22

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 199.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Arya D, Bhatt D, Ravi Kumar R, Tewari LM, Kishor K, Joshi GC (2013) Studies on natural resources, trade and conservation of Kutki (Picrorhiza kurroa Royle ex Benth, Scrophulariaceae) from Kumaun Himalaya. Sci Res Essays 8:575–580

    Google Scholar 

  • Baruah CC, Gupta PP, Nath A, Patnaik LG, Dhawan BN (1988) Anti-allergic and anti-anaphylactic activity of picroliv—a standardised iridoid glycoside fraction of Picrorhiza kurroa. Pharma Res 36:487–492

    Google Scholar 

  • Benson EE (2008) Cryopreservation of phytodiversity: a critical appraisal of theory & practice. Crit Rev Plant Sci 27:141–219

    Article  CAS  Google Scholar 

  • Bhat WW, Lattoo SK, Rana S, Razdan S, Dhar N, Dhar RS, Vishwakarma RA (2012) Efficient plant regeneration via direct organogenesis and Agrobacterium tumefaciens-mediated genetic transformation of Picrorhiza kurroa: An endangered medicinal herb of the alpine Himalayas. In Vitro Cell Dev Biol Plant 48:295–303

    Article  CAS  Google Scholar 

  • Bodeker G, Klooster VT, Weisbord E (2014) Prunus africana (Hook.f.) Kalkman: the overexploitation of a medicinal plant species and its legal context. J Altern Complement Med 20:810–822

    Article  Google Scholar 

  • Chand D, Malik ZA, Nautiyal MC (2016) Conservation of Picrorhiza kurrooa through cultivation in Garhwal Himalaya: a review. Int J Herb Med 4:64–69

    Google Scholar 

  • Chandra B, Palni LMS, Nandi SK (2006) Propagation and conservation of P. kurrooa Royle ex Benth: an endangered Himalayan medicinal herb of high commercial value. Biodiver Conserv 15:2325–2328

    Article  Google Scholar 

  • Chen SL, Yu H, Luo HM, Wu Q, Li CF, Steinmetz A (2016) Conservation and sustainable use of medicinal plants: problems, progress, and prospects. Chin Med 11:37

    Article  Google Scholar 

  • Gupta S, Chaturvedi P, Joshi K. Micropropagation (2016) Bioaugmentation of Picroside I in Leaves of in vitro cultured plants of Picrorhiza kurrooa Royle ex Benth. Nat Acad Sci Lett 39. https://doi.org/10.1007/s40009-016-0443-3

  • Helena DS, Kannojia G, Gaur AK (2015) In vitro regeneration of Picrorhiza kurroa Royal ex Benth. for ex situ conservation and sustainable utilization. Ann Biol Res 6:7–14

    CAS  Google Scholar 

  • Hosseinzadeh S, Jafarikukhdan A, Hosseini A, Armand R (2015) The application of medicinal plants in traditional and modern medicine: a review of Thymus vulgaris. Int J Clin Med 6:635–642

    Article  Google Scholar 

  • Jan A, Phalisteen S, Thomas GT, Shawl AS (2009) Auxin prompted improved micropropagation protocol of Picrorhiza kurroa: an endangered medicinal plant. Plant Tissue Cult Biotech 19:161–167

    Google Scholar 

  • Kashyap SR (1970) Samhita or Vriddha Jivakiya Tantra. (Shastri IGM, trans). Bombey Sastu Sahitya

    Google Scholar 

  • Khanna PK, Kumar A, Chandra R, Verma V (2013) Germination behaviour of seeds of Withania somnifera (L.) Dunal: a high value medicinal plant. Physiol Mol Biol Plants 19:449–454

    Article  CAS  Google Scholar 

  • Kumar HM, Ramesh C (2014) Anticancer activity of nano encapsulated formulation from the extracts of Picrorhiza kurroa against human cancer cell lines. J Pharmacognosy Phytochem 2:182–185

    Google Scholar 

  • Lal N, Ahuja PS (1996) Plantlet regeneration from callus in Picrorhiza kurrooa Royle ex Benth.—an endangered medicinal plant. Plant Tissue Cult 6:127–134

    Google Scholar 

  • Lal N, Ahuja PS, Kukreja AK, Pandey B (1988) Clonal propagation of Picrorhiza kurroa royle ex Benth. by shoot tip culture. Plant Cell Rep 7:202–205

    Article  CAS  Google Scholar 

  • Mahajan R, Kapoor N, Singh I (2016) Somatic embryogenesis and callus regeneration in Picrorhiza kurroa Royle ex Benth. J Exp Biol Agric Sci 4:201–209

    CAS  Google Scholar 

  • Mallick MN, Singh M, Parveen R, Khan W, Ahmad S, Najm MZ, Husain SA (2015) HPTLC analysis of bioactivity guided anticancer enriched fraction of hydroalcoholic extract of Picrorhiza kurroa. Biomed Res Int 2015:513875

    Article  Google Scholar 

  • Mishra J, Bhandari H, Singh M, Rawat S, Agnihotri RK, Mishra S, Purohit S (2011) Hairy root culture of Picrorhiza kurroa Royle ex Benth.: a promising approach for the production of picrotin and picrotoxinin. Acta Physiol Plant 33:1841–1846

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473

    Article  CAS  Google Scholar 

  • Nadeem M, Palni LMS, Purohit AN, Pandey H, Nandi SK (2000) Propagation and conservation of Podophyllum hexandrum Royle: an important medicinal herb. Biol Conserv 92:121–129

    Article  Google Scholar 

  • Nautiyal MC (1988) Germination studies on some high altitude medicinal plant species. In: Proceedings of indigenous medicinal plant symposium. Today and Tomorrows Printers and Publishers, New Delhi, 1988, pp 107–112

    Google Scholar 

  • Nautiyal BP, Prakash V, Chauhan RS, Purohit H, Nautiyal MC (2001) Assessment of germinability, productivity and cost benefit analysis of Picrorhiza kurrooa cultivated at lower altitudes. Curr Sci 81:579–585

    Google Scholar 

  • Ochoa-Villarreal M, Howat S, Hong S, Jang MO, Jin YW, Lee EK, Loake GJ (2016) Plant cell culture strategies for the production of natural products. BMB Rep 49:149–158

    Article  CAS  Google Scholar 

  • Padulosi S, Leaman D, Quek P (2002) Challenges and opportunities in enhancing the conservation and use of medicinal and aromatic plants. J Herbs Spices Med Plants 9:243–267

    Article  Google Scholar 

  • Pan SY, Litscher G, Gao SH, Zhou SF, Yu ZL, Chen HQ, Zhang SF, Tang MK, Sun JN, Ko KM (2014) Historical perspective of traditional indigenous medical practices: the current renaissance and conservation of herbal resources. Evid Based Complement Altern Med 2014:525340

    Google Scholar 

  • Patial V, Devi K, Sharma M, Bhattacharya A, Ahuja PS (2012) Propagation of Picrorhiza kurroa Royle ex Benth.: an important medicinal plant of Western Himalaya. J Med Plant Res 6:4848–4860

    Article  CAS  Google Scholar 

  • Patial V, Sharma M, Bhattacharya A (2016) Potential of thidiazuron in improved micropropagation of Picrorhiza kurroa—an endangered medicinal herb of alpine Himalaya. Plant Biosyst 1–8

    Google Scholar 

  • Patil AA, Sachin BS, Shinde DB, Wakte PS (2013) Supercritical CO2 assisted extraction and LC-MS identification of picroside I and picroside II from Picrorhiza kurroa. Phytochem Anal 24:97–104

    Article  CAS  Google Scholar 

  • Pradhan S (2011) Antihyperglycemic effect of various medicinal plants of Sikkim Himalayas—a review. Int J Res Phytochem Pharma. 1:124–130

    CAS  Google Scholar 

  • Rawat B, Rawat JM, Mishra S, Mishra SN (2013a) Picrorhiza kurrooa: current status and tissue culture mediated biotechnological interventions. Acta Physiol Plant 35:1–12

    Article  CAS  Google Scholar 

  • Rawat B, Rawat JM, Mishra S, Mishra SN (2013b) Picrorhiza kurrooa: current status and tissue culture mediated biotechnological interventions. Acta Physiol Plant 35:1–12

    Article  CAS  Google Scholar 

  • Rawat JM, Rawat B, Mishra S, Bhandari A, Agnihotri RK, Chandra A (2016) Influence of Agrobacterium rhizogenes strains, acitosyringone, inoculum size and temperature on production of active ingredients from Picrorhiza kurrooa. Physiol Mol Biol Plants 22:423

    Article  CAS  Google Scholar 

  • Sharma N, Sharma B (2003) Cryopreservation of shoot tips of Picrorhiza kurrooa Royle ex Benth., an indigenous endangered medicinal plant through vitrification. Cryo Lett 24:181–190

    Google Scholar 

  • Sharma S, Katoch V, Rathour R, Sharma TR (2010) In vitro propagation of endangered temperate Himalayan medicinal herb Picrorhiza kurrooa Royle ex Benth. using leaf explants and nodal segments. J Plant Biochem Biotechnol 19:111–114

    Article  CAS  Google Scholar 

  • Sood H, Chauhan RS (2009) High frequency callus induction and plantlet regeneration from different explants of Picrorhiza kurroa—a medicinal herb of Himalayas. Afr J Biotech 8:1965–1972

    CAS  Google Scholar 

  • Sood H, Chauhan RS (2010) Biosynthesis and accumulation of a medicinal compound, Picroside-I, in cultures of Picrorhiza kurroa Royle ex Benth. Plant Cell Tiss 100:113–117

    Article  CAS  Google Scholar 

  • Srivastava M, Sharma S, Misra P (2016) Elicitation based enhancement of secondary metabolites in Rauwolfia serpentina and Solanum khasianum hairy root cultures. Pharmacognosy Mag 12(Suppl 3):S315–S320

    Google Scholar 

  • Sultan P, Jan A, Pervaiz Q (2016) Phytochemical studies for quantitative estimation of iridoid glycosides in Picrorhiza kurroa Royle. Bot Stud Int J 57:7

    Article  Google Scholar 

  • Thakur RS, Puri HS, Hussain A (1989) Major medicinal plants of India. CIMAP, Lucknow, pp 404–407

    Google Scholar 

  • Tiwari SS, Pandey MM, Srivastava S, Rawat AK (2012) TLC densitometric quantification of picrosides (picroside-I and picroside-II) in Picrorhiza kurroa and its substitute Picrorhiza scrophulariiflora and their antioxidant studies. Biomed Chromatogr 26:61–68

    Article  CAS  Google Scholar 

  • Trivedi P, Pandey A (2007) Biological hardening of micropropagated Picrorhiza kurrooa Royel ex Benth., an endangered species of medical importance. World J Microbiol Biotechnol 23:877–878

    Article  Google Scholar 

  • Vasava DS (2016) Application of biotechnological techniques for medicinal plant research. Forest Res 5:4

    Article  Google Scholar 

  • Verma P, Mathur AK, Jain SP, Mathur A (2012) In vitro conservation of twenty-three over exploited medicinal plants belonging to the Indian sub continent. SciI World J 2012:929650

    Google Scholar 

Download references

Acknowledgements

The authors are thankful to National Medicinal Plant Board (NMPB), New Delhi for their financial support as a major project. The authors are also thankful to School of Biotechnology, University of Jammu, Jammu.

Author information

Authors and Affiliations

  1. School of Biotechnology, University of Jammu, Jammu, J&K, India

    Ritu Mahajan & Nisha Kapoor

Authors
  1. Ritu Mahajan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nisha Kapoor
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Medicinal Plants, Arak University, Arak, Iran

    Mansour Ghorbanpour

  2. Amity Institute of Microbial Technology, Amity University, Noida, Uttar Pradesh, India

    Ajit Varma

Rights and permissions

Reprints and permissions

Copyright information

© 2017 Springer International Publishing AG

About this chapter

Cite this chapter

Mahajan, R., Kapoor, N. (2017). Biotechnological Approaches for In Vitro Conservation of Picrorhiza kurroa Royle ex Benth. In: Ghorbanpour, M., Varma, A. (eds) Medicinal Plants and Environmental Challenges. Springer, Cham. https://doi.org/10.1007/978-3-319-68717-9_21

Download citation

Publish with us

Policies and ethics

Search

Navigation