Proteomic Research in Urine and Other Fluids

Isani, Gloria; Ferlizza, Enea; Nally, Jarlath E.

doi:10.1007/978-3-319-69682-9_7

Gloria Isani⁴,
Enea Ferlizza⁴ &
Jarlath E. Nally⁵

1205 Accesses
1 Citations

Abstract

Urine is an ideal biological sample. It is an important source of clinical biomarkers of systemic and renal diseases and can be obtained repeatedly without causing stress. Different analytical protocols for the study of the urine proteome have been used, from the simplest electrophoresis on agarose gels to the most sensitive and complex 2DE. As with urine, a range of bodily fluids (saliva, cerebrospinal, bronchoalveolar, amniotic fluids) are amenable to the tools of proteomics. The identification and characterization of the protein content of such fluids are being used to provide novel insights into the evolutionary adaptations of farm and domestic animal species and to characterize their normal physiological state. A comprehensive review on the application of proteomics to these biological samples is presented.

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Abbreviations

2DE:: Two-dimensional electrophoresis
Ig:: Immunoglobulins
MS:: Mass spectrometry
AKI:: Acute kidney injury
DIGE:: Difference gel electrophoresis
BSE:: Bovine spongiform encephalopathy
AGE:: Agarose gel electrophoresis
CAM:: Cellulose acetate membrane
HRE:: High-resolution electrophoresis
PrPC:: Physiological prion protein
GO:: Gene ontology
CSF:: Cerebrospinal fluid
BALF:: Bronchoalveolar fluid

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Authors and Affiliations

Department of Veterinary Medical Sciences (DIMEVET), Alma Mater Studiorum-University of Bologna, Bologna, Italy
Gloria Isani & Enea Ferlizza
Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, Agricultural Research Service (ARS), United States Department of Agriculture (USDA), Ames, IA, USA
Jarlath E. Nally

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Gloria Isani
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Enea Ferlizza
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Jarlath E. Nally
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Correspondence to Gloria Isani .

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Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, Lisbon, Portugal
Andre Martinho de Almeida
ERA Chair Laboratory, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
David Eckersall
Institute of Medical Biochemistry, University of Veterinary Medicine, Vienna, Austria
Ingrid Miller

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Isani, G., Ferlizza, E., Nally, J.E. (2018). Proteomic Research in Urine and Other Fluids. In: de Almeida, A., Eckersall, D., Miller, I. (eds) Proteomics in Domestic Animals: from Farm to Systems Biology. Springer, Cham. https://doi.org/10.1007/978-3-319-69682-9_7

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DOI: https://doi.org/10.1007/978-3-319-69682-9_7
Published: 02 February 2018
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-69681-2
Online ISBN: 978-3-319-69682-9
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