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Diaphanization Techniques in the Study of Root Canal Anatomy

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The Root Canal Anatomy in Permanent Dentition

Abstract

The study of the root canal anatomy is very important in endodontics. The introduction of innovative technologies such as micro-CT or CBCT has provided a great advance in the field; however, due to their excessive costs, it is still out of reach of most practitioners and researchers. Dental diaphanization or transparency methods, also known as dental clarification, are economical in their implementation, and the results are reliable. These features make this technique a useful tool for understanding the internal anatomy of human teeth while providing personal assessment of various endodontic procedures using extracted teeth. The mastery of advanced dental transparency techniques can require a personal learning curve where art and trial and error play a key role. Practice, patience, and perseverance are essential elements in learning these processes, but the basic elements and fundamentals of tooth transparency can give results that allow the teaching and learning benefits that are offered herein. Therefore, this chapter aimed to describe the foundation of the dental diaphanization methodology and provide details about the most used techniques.

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References

  1. Spalteholz KW. Über das Durchsichtigmachen von menschlichen und tierischen Präparaten, nebst Anhang: Über Knochenfärbung. Leipzig: S. Hirzel; 1911.

    Google Scholar 

  2. Spalteholz KW, inventor. Manufacture of transparent and translucent bodies. Patent number US1021952A.1912.

    Google Scholar 

  3. Prinz H. The Spalteholz method of preparing transparent animal bodies. Dental Cosmos. 1913;55:374–8.

    Google Scholar 

  4. Fasoli, Arlotta. Sull’anatomia dei canali radicolari dei denti umani. Giugno: Stomatologia; 1913.

    Google Scholar 

  5. Vertucci FJ. Root canal anatomy of the human permanent teeth. Oral Surg Oral Med Oral Pathol. 1984;58:589–99.

    Article  Google Scholar 

  6. Altunbaş D, Kuştarci A, Arslan D, Er K, Kocak S. Comparison of various current electronic apex locators to determine the working length using the clearing technique. Niger J Clin Pract. 2015;18:359–63.

    Article  Google Scholar 

  7. Venturi M, Prati C, Capelli G, Falconi M, Breschi L. A preliminary analysis of the morphology of lateral canals after root canal filling using a tooth-clearing technique. Int Endod J. 2003;36:54–63.

    Article  Google Scholar 

  8. Venturi M, Di Lenarda R, Prati C, Breschi L. An in vitro model to investigate filling of lateral canals. J Endod. 2005;31:877–81.

    Article  Google Scholar 

  9. de Gregorio C, Estevez R, Cisneros R, Heilborn C, Cohenca N. Effect of EDTA, sonic, and ultrasonic activation on the penetration of sodium hypochlorite into simulated lateral canals: an in vitro study. J Endod. 2009;35:891–5.

    Article  Google Scholar 

  10. de Gregorio C, Estevez R, Cisneros R, Paranjpe A, Cohenca N. Efficacy of different irrigation and activation systems on the penetration of sodium hypochlorite into simulated lateral canals and up to working length: an in vitro study. J Endod. 2010;36:1216–21.

    Article  Google Scholar 

  11. Robertson D, Leeb IJ, McKee M, Brewer E. A clearing technique for the study of root canal systems. J Endod. 1980;6:421–4.

    Article  Google Scholar 

  12. Robertson DC, Leeb IJ. The evaluation of a transparent tooth model system for the evaluation of endodontically filled teeth. J Endod. 1982;8:317–21.

    Article  Google Scholar 

  13. Yamamoto T, Domon T, Takahashi S, Islam MN, Suzuki R. A resin embedding method for transparent teeth with ink-infiltrated pulp cavities. Ann Anat. 2001;183:481–3.

    Article  Google Scholar 

  14. Omer OE, Al Shalabi RM, Jennings M, Glennon J, Claffey NM. A comparison between clearing and radiographic techniques in the study of the root-canal anatomy of maxillary first and second molars. Int Endod J. 2004;37:291–6.

    Article  Google Scholar 

  15. Weng XL, Yu SB, Zhao SL, Wang HG, Mu T, Tang RY, et al. Root canal morphology of permanent maxillary teeth in the Han nationality in Chinese Guanzhong area: a new modified root canal staining technique. J Endod. 2009;35:651–6.

    Article  Google Scholar 

  16. Neelakantan P, Subbarao C, Subbarao CV. Comparative evaluation of modified canal staining and clearing technique, cone-beam computed tomography, peripheral quantitative computed tomography, spiral computed tomography, and plain and contrast medium-enhanced digital radiography in studying root canal morphology. J Endod. 2010;36:1547–51.

    Article  Google Scholar 

  17. Lee KW, Kim Y, Perinpanayagam H, Lee JK, Yoo YJ, Lim SM, et al. Comparison of alternative image reformatting techniques in micro-computed tomography and tooth clearing for detailed canal morphology. J Endod. 2014;40:417–22.

    Article  Google Scholar 

  18. Monika, Dhawan R, Dhawan S, Mehta P. Analysis of root canal anatomy & morphological variations of maxillary 1st molar by different methods – an in vitro study. Endodontology. 2014;26:279–85.

    Google Scholar 

  19. Rehman K, Khan FR, Habib S. Diaphonization: a recipe to study teeth. J Contemp Dent Pract. 2015;16:248–51.

    Article  Google Scholar 

  20. Barrington C. Clearing a tooth to expose internal anatomy. Endod Practice USA. 2015;8:18–23.

    Google Scholar 

  21. Greco-Machado Y, García-Molina JA, Bueno-Martínez R, Manzanares-Céspedes MC, Lozano-De Luaces V. Técnicas de diafanización: estudio comparativo. Endodoncia. 2008;26:85–92.

    Google Scholar 

  22. Okumura T. Anatomy of the root canals. JADA. 1927;14:632–6.

    Google Scholar 

  23. Aprile H, Figún ME, Garino RR. Anatomía odontológica: orocervicofacial. 4th ed. Buenos Aires: El Ateneo; 1967.

    Google Scholar 

  24. Gómez ME, Campos A. Histología, embriología e ingeniería tisular bucodental. 3rd ed. Mexico City: Medica Panamericana; 2010.

    Google Scholar 

  25. An YH, Martin KL. Handbook of histology methods for bone and cartilage. 1st ed. New York: Humana Press; 2003.

    Google Scholar 

  26. Segura-Egea J, Jimenez-Rubio A, Llamas R, Jimenez-Planas A. El ácido etilen diamino tetraacético (EDTA) y su uso en endodoncia. Endodoncia. 1997;15:90–7.

    Google Scholar 

  27. IHC World: Life science product and services. Methods and protocols for decalcification of bone materials https://www.ihcworld.com/_protocols/histology/decalcification.htm. Accessed 12 May 2018.

  28. Mawhinney WH, Richardson E, Malcolm AJ. Control of rapid nitric acid decalcification. J Clin Pathol. 1984;37:1409–13.

    Article  Google Scholar 

  29. Suvarna K, Layton C, Bancroft JD. Bancroft’s theory and practice of histological techniques. 7th ed. Churchill, Livingstone: Elsevier; 2013.

    Google Scholar 

  30. Chang R, Goldsby K. Química. 11th ed. McGraw-Hill: Mexico City; 2015.

    Google Scholar 

  31. Ebbing D, Gammon SD. General chemistry. 11th ed. Auburn, WA: Cengage Learning; 2016.

    Google Scholar 

  32. Pécora JD, Woelfel JB, Sousa-Neto MD, Issa EP. Morphologic study of the maxillary molars. Part II: Internal anatomy. Braz Dent J. 1992;3:53–7.

    Google Scholar 

  33. Cediel J, Cárdenas M, García A. Manual de histología: tejidos fundamentales. 1st ed. Rosario, Argentina: Editorial Universidad de Rosario; 2009.

    Google Scholar 

  34. Agency for Toxic Substances and Disease Registry. Public health statement for 1,4 dioxane CAS # 123-91-1, Atlanta, GA, 2015. https://www.atsdr.cdc.gov/ToxProfiles/tp187-c1-b.pdf. Accessed 12 May 2018.

  35. Agency for Toxic Substances and Disease Registry. ToxFAQs for chloroform CAS # 67-66-3, Atlanta, GA, 2011. https://www.atsdr.cdc.gov/toxfaqs/tfacts6.pdf. Accessed 12 May 2018.

  36. Verdín S, Moreno L, Rojo N, García A, Omaña M, Meneses A, et al. Histología e inmunohistoquímica: Manual de métodos. 1st ed. Mexico City: Editorial de la Universidad Nacional Autónoma de México, Facultad de Estudios Superiores Iztacala; 2013.

    Google Scholar 

  37. Center for Disease Control and Prevention. The National Institute for Occupational Safety and Health. Isopropyl alcohol, 2015. https://www.cdc.gov/niosh/ipcsneng/neng0554.html. Accessed 12 May 2018.

  38. Pécora JD, Silva RS, Sousa-Neto MD. Apresentação de uma técnica simplificada de diafanização de dentes e sua inclusão em blocos transparentes. Odonto. 1993;2:384–5.

    Google Scholar 

  39. Evans D, Steele R, Sumnar M. Animal litter comprising gypsum and aluminum sulfate and processes of making same, US 6029603A, 2000. https://www.google.com/patents/US6029603. Accessed 12 May 2018.

  40. Wilse Robinson G, Cho CH, Gellene GI. Refractive index mysteries of water. J Phys Chem B. 2000;104:7179–82.

    Article  Google Scholar 

  41. Meng Z, Yao XS, Yao H, Liang Y, Liu T, Li Y, et al. Measurement of the refractive index of human teeth by optical coherence tomography. J Biomed Opt. 2009;14:034010.

    Article  Google Scholar 

  42. Armarego WLF, Perrin DD. Purification of laboratory chemicals. 4th ed. Boston/Oxford: Butterworth Heinemann; 1997.

    Google Scholar 

  43. Chan TY. The risk of severe salicylate poisoning following the ingestion of topical medicaments or aspirin. Postgrad Med J. 1996;72:109–12.

    Article  Google Scholar 

  44. Chin RL, Olson KR, Dempsey D. Salicylate toxicity from ingestion and continued dermal absorption. Cal J Emerg Med. 2007;8:23–5.

    PubMed  PubMed Central  Google Scholar 

  45. Keller O. Untersuchungen zur Anatomie der Wurzelkanäle des menschlichen Gebisses nach dem Aufhellungsverfahren. Zurich; 1928.

    Google Scholar 

  46. Castelucci A. Endodontics. Edizioni Odontoiatriche Il Tridente: Florence; 2005.

    Google Scholar 

  47. Malentacca A, Lajolo C. A new technique to make transparent teeth without decalcifying: description of the methodology and micro-hardness assessment. Ann Anat. 2015;197:11–5.

    Article  Google Scholar 

  48. von Stetten ZAO, Vahedi B, Reuver H. Der transparente Zahn. Endod J. 2008;3:34–5.

    Google Scholar 

  49. Thompson R. Close-up and macro photography: its art and field craft techniques. New York: Focal Press; 2017.

    Google Scholar 

  50. Augerson CC, Messinger JM. Controlling the refractive index of epoxy adhesives with acceptable yellowing after aging. JAIC. 1993;32:311–4.

    Google Scholar 

  51. Mark JE. Physical properties of polymers handbook. New York: Springer Science & Business Media; 2007.

    Book  Google Scholar 

  52. Hasselgren G, Tronstad L. The use of transparent teeth in the teaching of preclinical endodontics. J Endod. 1975;1:278–80.

    Article  Google Scholar 

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Author information

Authors and Affiliations

  1. Private Practice, Waxahachie, TX, USA

    Craig Barrington D.D.S.

  2. Private Practice Limited to Endodontics, Torreón, Coahuila, Mexico

    Francisco Balandrano D.D.S.

Authors
  1. Craig Barrington D.D.S.
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  2. Francisco Balandrano D.D.S.
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Corresponding author

Correspondence to Craig Barrington D.D.S. .

Editor information

Editors and Affiliations

  1. University of São Paulo, Ribeirão Preto, Brazil

    Marco A. Versiani

  2. University of Toronto, Toronto, Canada

    Bettina Basrani

  3. University of São Paulo, Ribeirão Preto, Brazil

    Manoel D. Sousa-Neto

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Barrington, C., Balandrano, F. (2019). Diaphanization Techniques in the Study of Root Canal Anatomy. In: Versiani, M., Basrani, B., Sousa-Neto, M. (eds) The Root Canal Anatomy in Permanent Dentition. Springer, Cham. https://doi.org/10.1007/978-3-319-73444-6_5

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  • DOI: https://doi.org/10.1007/978-3-319-73444-6_5

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  • Online ISBN: 978-3-319-73444-6

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