Abstract
Across the globe nitrogen is limiting primary productivity. Although fertilizer nitrogen could supplement soil nitrogen depletion, efforts should be to augment soil biological nitrogen fixation mediated by microbes. This could be addressed by conservation and sustainable use of rhizobia that can fix nitrogen in soil in association with legumes. Rhizobia commonly occur in soils but often fail to produce effective nodulation either because their population in soil is low or those present cannot effectively nodulate the particular legume. Rhizobia present in various ecosystems are considerably diverse; at present 130 species within 15 genera are reported. Rhizobial inoculation is almost always needed when certain new leguminous crops are introduced to new areas or when ineffective and incompatible rhizobia are present in the soils. For this, depository of authentic microbial gene pool is a must. Culture collection centers act as repository of valuable microbial strains. In the changing global scenario, these centers are becoming Biological Resource Centres (BRCs), carry out research, enhance value of strains, and control access to dangerous microorganisms. For adoption and popularizing use of rhizobium-legume technology, their isolation, identification, preservation, and deposition in biological banks are imminent. Moreover, there is a need to identify the compatible rhizobium-legume symbioses for sustainable agriculture.
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References
Amarger N, Macheret V, Laguerre G (1997) Rhizobium gallicum sp. nov. and Rhizobium giardinii sp. nov., from Phaseolus vulgaris nodules. Int J Syst Bacteriol 47:996–1006
Anonymous (2017) India to overtake China’s population in 7 yrs. A UN report published in Times of India
Australian Inoculant Research and Control Service, New South Wales Department of Agriculture Melbourne. http://www.dpi.nsw.gov.au/
Azevedo H, Lopes FM, Silla PR, Hungria M (2015) A database for the taxonomic and phylogenetic identification of the genus Bradyrhizobium using multilocus sequence analysis. BMC Genomics 16:S10
Ba S, Willems A, de Lajudie P, Roche P, Jeder H, Quatrini P, Neyra M, Ferro M, Prome JC, Gillis M, Boivin-Masson C, Lorquin J (2002) Symbiotic and taxonomic diversity of rhizobia isolated from Acacia tortilis subsp. Raddiana in Africa. Syst Appl Microbiol 25:130–145
Campos AK, de Andrade Mota M, de Araújo JV, Cecon PR (2004) Predatory activity, radial growth and sporulation of fungi predating Monacrosporium nematode submitted to cryopreservation. Sci Rural 34:465–469
Commonwealth Scientific and Industrial Research Organization’s (CSIRO) Cunningham Laboratory, Brisbane. https://www.csiro.au/en/Publications
Cummings SP, Gyaneshwar P, Vinuesa P, Farruggia FT, Andrews M, Humphry D, Elliott GN, Nelson A, Orr C, Pettitt D, Shah GR, Santos SR, Krishnan HB, Odee D, Moreira FMS, Sprent JI, Young JPW, James EK (2009) Nodulation of Sesbania species by Rhizobium (Agrobacterium) strain IRBG74 and other rhizobia. Environ Microbiol 11:2510–2525
Day JG, Stacey GN (eds) (2007) Cryopreservation and freeze-drying protocols, 2nd edn. Methods in molecular biology series 368. Humana, Totowa
Denardin ND, Freire JRJ (2000) Assessment of polymers for the formulation of legume inoculants. World J Microbiol Biotechnol 16:215–217
Fernandes Júnior PI, Rohr TG, de Oliveira PJ, Xavier GR, Rumjanek NG (2009) Polymers as carriers for rhizobia inoculant formulations. Pesq Agropec Bras Brasília 44:1184–1190
Frank B (1889) Über die Pilzsymbiose der Leguminosen. Ber Dtsch Bot Ges. 7:332–346
Fred EB, Baldwin IL, McCoy E (1932) Root nodule bacteria and leguminous plants. University of Wisconsin. Digitized19 Feb 2008
Freire JRJ, Kolling I (1986) World culture collections of Rhizobium. World J Microbiol Biotechnol 2:231–235
Gerhardt P, Murray RGE, Costilow RN, Nester EW, Wood WA, Krieg NR, Phillips GB (eds) (1981) Manual of methods for general bacteriology. American Society of Microbiology, Washington
Gevers D, Cohan FM, Lawrence JG, Spratt BG, Coenye T, Feil EJ, Stackebrandt E, de Peer YV, Vandamme P, Thompson FL, Swings J (2005) Re-evaluating prokaryotic species. Nat Rev Microbiol 3:733–739
Graham PH, Sadowsky MJ, Kersters HH, Barnet YM, Bradley RS, Cooper JE, De Ley DJ, Jarvis BDW, Roslycky EB, Strijdom BW, Young JPW (1991) Proposed minimal standards for the description of new genera and species of root-and stem-nodulating bacteria. Int J Syst Bacteriol 41:582–587
Haukka K, Lindstrom K, Young JPW (1998) Three phylogenetic groups of nodA and nifH genes in Sinorhizobium and Mesorhizobium isolates from leguminous trees growing in Africa and Latin America. Appl Environ Microbiol 64:419–426
Howeison JG, Dilworth MJ (2016) Working with rhizobia. Australian Centre for International Agricultural research, Canberra
Hubálek Z (2003) Protectants used in the cryopreservation of microorganisms. Cryobiology 46:205–229
Hungria M, Ardley J, O’Hara GW, Howeison JG (2016) Preservation of rhizobia. In: Howeison JG and Dilworth MJ (eds) Working with rhizobia. Australian Centre for International Agricultural Research, Canberra, pp 61–72
Johnson MD, Allen ON (1952) Cultural reactions of rhizobia with special reference to strains isolated from Sesbania species. Ant Leewenhoek 18:1–12
Jordan DC (1982) Notes: transfer of Rhizobium japonicum Buchanan 1980 to Bradyrhizobium gen. Nov., a genus of slow-growing, root nodule bacteria from leguminous plants. Int J Syst Bacteriol 32(1):136–139
Jordan DC (1984) Family III. Rhizobiaceae Conn (1938). In: Krieg NR, Holt JG (eds) Bergey’s manual of systematic bacteriology, vol 1. Williams and Wilkins, Baltimore, pp 234–254
Kampfer P, Glaeser SP (2012) Prokaryotic taxonomy in the sequencing era the polyphasic approach revisited. Environ Microbiol 14:291–317
Konstantinidis K, Tiedje JM (2005a) Genomic insights that advance the species definition for prokaryotes. Proc Natl Acad Sci USA 102:2567–2572
Konstantinidis KT, Tiedje JM (2005b) Towards a genome-based taxonomy for prokaryotes. J Bacteriol 187:6258–6264
Leon-Barrios M, Lorite MJ, Donate-Correa J, Sanjuan J (2009) Ensifer meliloti bv. lancerottense establishes nitrogen-fixing symbiosis with Lotus endemic to the Canary Islands and shows distinctive symbiotic genotypes and host range. Syst Appl Microbiol 32:413–420
Lindström K, Young JP (2011) International Committee on Systematics of Prokaryotes Subcommittee on the taxonomy of Agrobacterium and Rhizobium: minutes of the meeting, 7 September 2010, Geneva, Switzerland. Int J Syst Evol Microbiol 61:3089–3093
Lortet G, Mear N Lorquin J, Dreyfus B, de Lajudie P, Rosenberg C, Boivin C (1996) Nod factor thin-layer chromatography profiling as a tool to characterize symbiotic specificity of rhizobial strains: application to Sinorhizobium saheli, S. teranga, and Rhizobium sp. strains isolated from Acacia and Sesbania. Mol Plant–Microbe Interact 9:736–747
Maatallah J, Berraho E, Munoz S, Sanjuan J, Lluch C (2002) Phenotypic and molecular characterization of chickpea rhizobia isolated from different areas of Morocco. J Appl Microbiol 93:531–540
Menna P, Hungria M (2011) Phylogeny of nodulation and nitrogen fixation genes in Bradyrhizobium: supporting evidence for the theory of monophyletic origin, and spread and maintenance by both horizontal and vertical transfer. Int J Syst Evol Microbiol 61:3052–3067
Menna P, Barcellos FG, Hungria M (2009) Phylogeny and taxonomy of a diverse collection of Bradyrhizobium strains based on multilocus sequence analysis of the 16S rRNA gene, ITS region and glnII, recA, atpD and dnaK genes. Int J Syst Evol Microbiol 59:2934–2950
Mnasri B, Mrabet M, Laguerre G, Aouani ME, Mhamdi R (2007) Salt-tolerant rhizobia isolated from a Tunisian oasis that are highly effective for symbiotic N2-fixation with Phaseolus vulgaris constitute a novel biovar (bv. mediterranense) of Sinorhizobium meliloti. Arch Microbiol 187:79–85
Nandasena KG, O’Hara GW, Tiwari RP, Sezmis E, Howieson JG (2007) In situ lateral transfer of symbiosis islands results in rapid evolution of diverse competitive strains of mesorhizobia suboptimal in symbiotic nitrogen fixation on the pasture legume Biserrula pelecinus L. Environ Microbiol 9:2496–2511
Nour SM, Cleyet Marel JC, Normand P, Fernandez MP (1995) Genomic heterogeneity of strains nodulating chickpeas (Cicer arietinum L.) and description of Rhizobium mediterraneum sp. nov. Int J Syst Bacteriol 45:640–648
OECD (2007) Promoting pro-poor growth: policy guidance for donors. OECD Publishing. https://doi.org/10.1787/9789264024786-en
Ormeño-Orrillo E, Servín-Garcidueñas LE, Rogel MA, González V, Peralta H, Mora J, Martínez-Romero J, Martínez-Romero E (2015) Taxonomy of Rhizobia and Agrobacteria from the Rhizobiaceae family in light of genomics. Syst Appl Microbiol 38:287–291
Peterson TA, Russelle MP (1991) Alfalfa and the nitrogen cycle in the Corn Belt. J Soil Water Conserv 46:229–235
Quispel A (1988) Hellriegel and Wilfarth’s discovery of (symbiotic) nitrogen fixation one hundred years ago. In: Bothe H, de Bruijn FJ, Newton WE (eds) Nitrogen fixation: one hundred years after. Gustav Fisher, Stuttgart, pp 3–12
Radeva G, Jurgens G, Niemi M, Nick G, Suominen L, Lindstrom K (2001) Description of two biovars in the Rhizobium galegae species: biovar orientalis and biovar officinalis. Syst Appl Microbiol 24:192–205
Ramírez-Bahena MH, García Fraile P, Peix A, Valverde A, Rivas R, Igual JM, Mateos PF, Martínez Molina E, Velázquez E (2008) Revision of the taxonomic status of the species Rhizobium leguminosarum (Frank 1879) Frank 1889AL, Rhizobium phaseoli Dangeard 1926AL and Rhizobium trifolii Dangeard 1926AL. R. trifolii is a later synonym of R. leguminosarum. Reclassification of the strain R. leguminosarum DSM 30132 (=NCIMB 11478) as Rhizobium pisi sp. nov. Int J Syst Evol Microbiol 58:2484–2490
Rivas R, Laranjo M, Mateos PF, Oliveira S, Martínez-Molina E, Velázquez E (2007) Strains of Mesorhizobium amorphae and Mesorhizobium tianshanense, carrying symbiotic genes of common chickpea endosymbiotic species, constitute a novel biovar (ciceri) capable of nodulating Cicer arietinum. Lett Appl Microbiol 44:412–418
Rogel MA, Ormeño-Orrillo E, Martinez Romero E (2011) Symbiovars in rhizobia reflects bacterial adaptation to legumes. Syst. Appl Microbiol 34:96–104. https://doi.org/10.1016/j.syapm.2010.11.015
Romeiro RS (2001) Methods in plant bacteriology. UFV, Viçosa, p 279
Sahgal M, Sharma A, Johri BN, Prakash A (2004) Selection of growth promotory rhizobia for Dalbergia sissoo from diverse soil ecosystems of India. Symbiosis 36:83–96
Sanz T, Fernández MA, Salvador A, Muñhoz J, Fiszman SM (2005) Thermogelation properties of methylcellulose (MC) and their effect on a batter formula. Food Hydrocoll 19:141–147
Segovia L, Young JPW, Martinez-Romero E (1993) Reclassification of American Rhizobium leguminosarum biovar phaseoli type I strains as Rhizobium etli sp. nov. Int J Syst Bacteriol 43:374–377
Silva C, Vinuesa P, Eguiarte LE, Souza V, Martinez-Romero E (2005) Evolutionary genetics and biogeographic structure of Rhizobium gallicum sensu lato, a widely distributed bacterial symbiont of diverse legumes. Mol Ecol 14:4033–4050
Sly LI (2010) Biodiversity and the role of microbial resource centres. In: Brown AG (ed) Proceedings of conference “biodiversity and world food security: nourishing the planet and its people”. Conducted by the Crawford Fund for International Agricultural Research, Parliament House, Canberra, Australia, 30 August–1 September, 2010, pp 60–67
Smith D (2003) Culture collections over the world. Int Microbiol 6:95–100
Stackebrandt E, Frederiksen W, Garrity GM, Grimont PAD, Kampfer P, Maiden X, Nesme MCJ, Rossello-Mora R, Swings J, Truper HG, Vauterin L, Ward AC, Whitman WB (2002) Report of the adhoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047
Terpolilli J, O’Hara GW, Tiwari RP, Dilworth MJ, Howieson JG (2008) The model legume Medicago truncatula A17 is poorly matched for N2 fixation with the sequenced microsymbiont Sinorhizobium meliloti 1021. New Phytol 179:62–66
Tian CF, Wang ET, Wu LJ, Han TX, Chen WF, Gu CT, Gu JG, Chen WX (2008) Rhizobium fabae sp. nov., a bacterium that nodulates Vicia faba. Int J Syst Evol Microbiol 58:2871–2875
Tilman D, Reich PB, Knops J, Wedin D, Mielke T, Lehman C (2001) Diversity and productivity in a long-term grassland experiment. Sci 294:843–845
Tindall BJ, Rosselló-Móra R, Busse H-J, Ludwig W, Kämpfer P (2010) Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 60:249–266
Trewavas A (2001) Urban myths of organic farming. Nature 410:409–410
Van Elsas JD (2001) Effects of ecological factors on the survival and physiology of Ralstonia solanacearum bv.2 in irrigation water. Can J Microbiol 47:842–854
Vandamme P, Pot B, Gillis M, De Vos P, Kersters K, Swings J (1996) Polyphasic taxonomy; a consensus approach to bacterial systematics. Microbiol Mol Biol Rev 60:407–438
Villegas MC, Rome S, Maure L, Domergue O, Gardan L, Bailly X, Cleyet- Marel JC, Brunel B (2006) Nitrogen-fixing sinorhizobia with Medicago laciniata constitute a novel biovar (bv. medicaginis) of S. meliloti. Syst Appl Microbiol 29:526–538
Vinuesa P, Leon-Barrios M, Silva C, Willems A, Jarabo-Lorenzo A, Perez-Galdona R, Werner D, Martinez-Romero E (2005) Bradyrhizobium canariense sp. nov., an acid-tolerant endosymbiont that nodulates endemic genistoid legumes (Papilionoideae: Genisteae) from the Canary Islands, along with Bradyrhizobium japonicum bv. genistearum, Bradyrhizobium genospecies alpha and Bradyrhizobium genospecies beta. Int J Syst Evol Microbiol 55:569–575
Wang ET, Rogel MA, Garcia-De los Santos A, Martinez-Romero J, Cevallos MA, Martinez-Romero E (1999) Rhizobium etli bv. mimosae, a novel biovar isolated from Mimosa affinis. Int J Syst Bacteriol 49:1479–1491
Weir BS (2016) The current taxonomy of rhizobia. NZ Rhizobia website. https://www.rhizobia.co.nz/taxonomy/rhizobia. Last updated: 10 Jan 2016
Willems A, Coopman R, Gillis M (2001) Comparison of sequence analysis of 16S–23S rDNA spacer regions, AFLP analysis and DNA–DNA hybridizations in Bradyrhizobium. Int J Syst Evol Microbiol 51:623–632
World Directory of Culture Collections, sixth version (2014) www.wfcc.info/ccinfo/index.php/home/statistics/#m2
Acknowledgments
The authors thank Indian Council of Agricultural Research (ICAR), and Ministry of Environment & Forests (MoEF), and GOI for financial assistance to MS. The senior author MS thanks Mariangela Hungria, Brazil for critical suggestions.
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Sahgal, M., Jaggi, V. (2018). Rhizobia: Culture Collections, Identification, and Methods of Preservation. In: Sharma, S., Varma, A. (eds) Microbial Resource Conservation. Soil Biology, vol 54. Springer, Cham. https://doi.org/10.1007/978-3-319-96971-8_6
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