Abstract
The velocity of the electroosmotic flow (EOF) is a crucial parameter for separations by capillary electrophoresis. It determines the range of analyte mobilities that can be handled in a single run as well as the run time. Moreover, variations in the EOF, between different capillaries and between different runs on the same capillary, are the main source of the irrepeatability and irreproducibility of the elution times so often encountered in CE. The electroosmotic mobility is in principle governed by the chemical condition of the inner surface of the capillary and by the composition of the background electrolyte (BGE) used for the separation. Still, even with careful control of these parameters, large variations in the EOF are found in practice.
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Kok, W. (2000). Capillaries and the Electroosmotic Flow. In: Capillary Electrophoresis: Instrumentation and Operation. Chromatographia CE-Series, vol 4. Vieweg+Teubner Verlag, Wiesbaden. https://doi.org/10.1007/978-3-322-83133-0_6
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DOI: https://doi.org/10.1007/978-3-322-83133-0_6
Publisher Name: Vieweg+Teubner Verlag, Wiesbaden
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