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Isolation and Regeneration of Protoplasts from Higher Plants

  • Chapter
Differentiation of Protoplasts and of Transformed Plant Cells

Part of the book series: Results and Problems in Cell Differentiation ((RESULTS,volume 12))

Abstract

The aseptic culture of plant cells has emerged in recent years as a powerful technique not only for the study of cell differentiation, but also for plant improvement and agriculture. During the past few years, the potential of plant cell culture has vastly improved due to the fast emerging technology of isolation, cultivation, and fusion, of protoplasts. The term “protoplast” in this respect refers to the spherical plasmolyzed contents of a plant cell enclosed in the plasmalemma and set free of the covering cell wall by a suitable experimental method. The naked cells so obtained constitute an ideal “free cell” developmental system because protoplasts are separate entities capable of reforming cell walls and regenerating whole plants. This fact is of considerable advantage as not only has clonal, large scale, propagation of desired plants become much more efficient, but being discrete, the protoplasts — especially from haploid plants or cell lines — can be handled like microbes and are well suited to mutagenic treatments and somatic cell genetics.

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References

  • Abo El-Nil MM, Hildebrandt AC (1976) Cell wall regeneration and colony formation from isolated single geranium protoplasts in microculture. Can J Bot 54: 1530–1534

    Google Scholar 

  • Ahuja PS, Hadiuzzaman S, Davey MR, Cocking EC (1983 a) Prolific plant regeneration from protoplast-derived tissue of Lotus corniculatus L. (birdsfoot trefoil). Plant Cell Rep 2: 101–104

    Google Scholar 

  • Ahuja PS, Lu DY, Cocking EC, Davey MR ( 1983 b) An assessment of the cultural capabilities of Trifolium repens L. (white clover) and Onobrychis viciifolia Scop. ( Sainfoin) mesophyll protoplasts. Plant Cell Rep 2: 269–272

    Google Scholar 

  • Akerman, KEO, Proudlove MO, Moore AL (1983) Evidence for a Ca++ gradient across the plasma membrane of wheat protoplasts. Biochem Biophys Res Commun 113: 171–177

    PubMed  CAS  Google Scholar 

  • Arcioni S, Davey MR, Santos AVP Dos, Cocking EC (1982) Somatic embryogenesis in tissues from mesophyll and cell suspension protoplasts of Medicago coerulea and M. glutinosa. Z Pflanzenphysiol 106: 105–110

    CAS  Google Scholar 

  • Arnold S von, Eriksson T (1976) Factors influencing the growth and division of pea mesophyll protoplasts. Physiol Plant 36: 193–196

    Google Scholar 

  • Arnold S von, Eriksson T (1977) A revised medium for growth of pea mesophyll protoplasts. Physiol Plant 39: 257–260

    Google Scholar 

  • Asamizu T, Nishi A (1980) Regenerated cell wall of carrot protoplasts isolated from suspension-cultured cells. Physiol Plant 48: 207–212

    CAS  Google Scholar 

  • Banks MS, Evans PK (1976) A comparison of the isolation and culture of mesophyll protoplasts from several Nicotiana species and their hybrids. Plant Sci Lett 7: 409–416

    Google Scholar 

  • Bapat VA, Schieder 0 (1981) Protoplast culture of several members of the genus Physalis. Plant Cell Rep 1: 69–70

    Google Scholar 

  • Barbier M, Dulieu H (1983) Early occurrence of genetic variants in protoplast cultures. Plant Physiol (Bethesda) 29: 201–206

    Google Scholar 

  • Barsby T, Shepard JF (1983) Regeneration of plants from mesophyll protoplasts of Solanum species of the Etuberosa group. Plant Sci Lett 31: 101–105

    Google Scholar 

  • Berry SF, Lu DY, Pental D, Cocking EC (1982) Regeneration of plants from protoplasts of Lactuca sativa L. Z Pflanzenphysiol 108: 31–38

    Google Scholar 

  • Bharal S, Rashid A (1980) Isolation of protoplasts from stem and hypocotyl of the legume Vigna sinensis and some factors affecting their regeneration. Protoplasma 102: 307–313

    Google Scholar 

  • Bhatt DP, Fassuliotis G (1981) Plant regeneration from mesophyll protoplasts of eggplant. Z Pflanzenphysiol 104: 81–89

    CAS  Google Scholar 

  • Bhojwani SS, Razdan MK (1983) Plant Tissue Culture: Theory and Practice. Elsevier, Amsterdam

    Google Scholar 

  • Bhojwani SS, Power JB, Cocking EC (1977) Isolation, culture and division of cotton callus protoplasts. Plant Sci Lett 8: 85–89

    CAS  Google Scholar 

  • Bidney DL, Shepard JF, Kaleikau E (1983) Regeneration of plants from mesophyll protoplasts of Brassica oleracea. Protoplasma 117: 89–92

    Google Scholar 

  • Bilkey PC, Cocking EC (1982) A non-enzymatic method for isolation of protoplasts from callus of Saintpaulia ionantha ( African violet ). Z Pflanzenphysiol 105: 285–288

    Google Scholar 

  • Binding H (1964) Regeneration und Verschmelzung nackter Laubmoosprotoplasten. Z Naturforsch Teil B Anorg Chem Org Chem 19: 775

    Google Scholar 

  • Binding H (1966) Regeneration und Verschmelzung nackter Laubmoosprotoplasten. Z Pflanzenphysiol 55: 305–321

    Google Scholar 

  • Binding H (1974 a) Cell cluster formation by leaf protoplasts from axenic cultures of haploid Petunia hybrida L. Plant Sci Lett 2:185–188

    Google Scholar 

  • Binding H (1974b) Regeneration von haploiden und diploiden Pflanzen aus Protoplasten von Petunia hybrida L. Z Pflanzenphysiol 74: 327–356

    Google Scholar 

  • Binding H (1984) Aufzucht von Pflanzen aus isolierten Protoplasten und Fusionskörpern. Abstr. In: Mitteilungsband — Kurzfassungen der Beiträge, Botaniker-Tagung in Wien 1984. Inst Bot Univ Wien, p 61

    Google Scholar 

  • Binding H, Kollmann R (1985, in press) Regeneration of protoplasts. In: Coordination of Agricultural Research. Seminar Proc. Comm Commun, Luxembourg

    Google Scholar 

  • Binding H, Nehls R (1977) Regeneration of isolated protoplasts to plants in Solanum dulcamara L. Z. Pflanzenphysiol 85: 279–280

    Google Scholar 

  • Binding H, Nehls R (1978) Regeneration of isolated protoplasts of Vicia faba L. Z Pflanzenphysiol 88: 327–332

    Google Scholar 

  • Binding H, Nehls R (1980) Protoplast regeneration to plants in Senecio vulgaris L. Z Pflanzenphysiol 99: 183–185

    Google Scholar 

  • Binding H, Nehls R, Schieder O, Sopory SK, Wenzel G (1978) Regeneration of mesophyll pro- toplasts isolated from dihaploid clones of Solanum tuberosum. Physiol Plant 43: 52–54

    Google Scholar 

  • Binding H, Nehls R, Kock R (1980) Versuche zur Protoplastenregeneration dikotyler Pflanzen unterschiedlicher systematischer Zugehörigkeit. Ber Dtsch Bot Ges 93: 667–671

    Google Scholar 

  • Binding H, Nehls R, Kock R, Finger J, Mordhorst G (1981) Comparative studies on protoplast regeneration in herbaceous species of the Dicotyledoneae class. Z Pflanzenphysiol 101: 119–130

    Google Scholar 

  • Blaschek W, Koehler H, Semler U, Franz G (1982) Molecular weight distribution of cellulose in primary cell walls. Investigations with regenerating protoplasts, suspension cultured cells and mesophyll of tobacco. Planta (Berl) 154: 550–555

    Google Scholar 

  • Bourgin JP, Missonier C (1978) Culture de protoplastes de mésophylle Nicotiana alata Link et Otto. Z Pflanzenphysiol 87: 55–64

    CAS  Google Scholar 

  • Bourgin JP, Missionier C, Chupeau Y (1976) Culture de protoplastes de mésophylle de Nicotiana sylvestris Spegazzini et Comes haplöíde et diplöíde. CR Acad Sci Paris Ser D 282: 1853–1856

    Google Scholar 

  • Bourgin JP, Chupeau Y, Missionier C (1979) Plant regeneration from mesophyll protoplasts of several Nicotiana species. Physiol Plant 45: 288–292

    CAS  Google Scholar 

  • Boyes DJ, Sink KC (1981) Regeneration of plants from callus-derived protoplasts of Salpiglossis. J Am Soc Hortic Sci 106: 42–46

    CAS  Google Scholar 

  • Boyes CJ, Zapata FJ, Sink KC (1980) Isolation, culture and regeneration to plants of callus protoplasts of Salpiglossis sinuata L. Z Pflanzenphysiol 99: 471–474

    CAS  Google Scholar 

  • Brar DS, Rambold S, Constabel F, Gamborg OL (1980) Isolation, fusion and culture of Sorghum and corn protoplasts. Z Pflanzenphysiol 96: 269–275

    Google Scholar 

  • Brenneman FN, Galston AW (1975) Experiments of the cultivation and calli of agriculturally important plants. 1. Oat (Avena sativa L.). Biochem Physiol Pflanzen (BPP) 168: 453–471

    CAS  Google Scholar 

  • Briskin DP, Leonard RT (1979) Ion transport and isolated protoplasts from tobacco suspension cells. III. Membrane potential. Plant Physiol (Bethesda) 64: 959–962

    Google Scholar 

  • Bui-Dang-Ha D, Mackenzie IA (1973) The division of protoplasts from Asparagus officinalis L. and their growth and differentiation. Protoplasma 78: 215–221

    Google Scholar 

  • Burgess J, Linstead PJ (1979) Structure and association of wall fibrils produced by regenerating tobacco protoplasts. Planta (Berl) 146: 203–210

    Google Scholar 

  • Butenko RG, Kuchko AA, Vitenko AA, Aventisov VA (1977) Production and cultivation of protoplasts isolated from the mesophyll of leaves of Solanum tuberosum L. and Solanum chacoense Bitt. Soy Plant Physiol (Engl Transl Fiziol Rast) 24: 540–541

    Google Scholar 

  • Caboche M (1980) Nutritional requirements of protoplast-derived, haploid tobacco cells grown at low cell densities in liquid medium. Planta (Berl) 149: 7–18

    CAS  Google Scholar 

  • Cai Q, Quain Y, Zhou Y, Wu S (1978) A further study on the isolation and culture of rice (Oryza sativa L.) protoplasts. Acta Bot Sin 20: 97–102

    Google Scholar 

  • Carlberg I, Glimelius K, Eriksson T (1983) Improved culture ability of potato protoplasts by use of activated charcoal. Plant Cell Rep 2: 223–225

    Google Scholar 

  • Chang T-Y, Senn AA, Pilet PE (1983) Effect of abscisic acid on maize root protoplasts. Z Pflanzenphysiol 110: 127–133

    CAS  Google Scholar 

  • Chapman KSR, Hatch MD (1983) Intracellular location of phosphoenol pyruvate carboxykinase and other C-4 photosynthetic enzymes in mesophyll and bundle sheath protoplasts of Panicum maximum. Plant Sci Lett 29: 145–154

    CAS  Google Scholar 

  • Chupeau Y, Bourgin JP, Missonier C, Dorion N, Morel G (1974) Preparation et culture de protoplastes de divers Nicotiana. CR Acad Sci Paris Ser D 278: 1564–1568

    Google Scholar 

  • Cocking EC (1960) A method for isolation of plant protoplasts and vacuoles. Nature (Lond) 187: 927–929

    Google Scholar 

  • Cocking EC (1966) Electron microscopic studies on isolated plant protoplasts. Z Naturforsch Teil B Anorg Chem Org Chem 21: 581–584

    Google Scholar 

  • Cocking EC (1972) Plant cell protoplasts — isolation and development. Annu Rev Plant Physiol 23: 29–50

    CAS  Google Scholar 

  • Constabel F, Kirkpatrick JW, Gamborg OL (1973) Callus formation from mesophyll protoplasts of Pisum sativum. Can J Bot 51: 2105–2106

    Google Scholar 

  • Cornel D, Grignon C, Rona JP, Heller R (1983) Measurement of intracellular potassium activity in protoplasts of Acer pseudoplatanus: origin of their electropositivity. Physiol Plant 57: 203–209

    CAS  Google Scholar 

  • Crepy L, Chupeau M-C, Chupeau Y (1982) The isolation and culture of leaf protoplasts of Cich-orium intybus and their regeneration into plants. Z Pflanzenphysiol 107: 123–131

    CAS  Google Scholar 

  • Davey MR, Bush E, Power JB (1974) Cultural studies of a dividing legume leaf protoplast sys-tem. Plant Sci Lett 3: 127–133

    Google Scholar 

  • Davey MR, Mathias RJ (1979) Close-packing of plasma membrane particles during wall regen- eration by isolated higher plant protoplasts — fact or artifact? Protoplasma 100: 85–99

    Google Scholar 

  • Deka PC, Sen SK (1976) Differentiation in calli originated from isolated protoplasts of rice (Oryza sativa L.) through plating technique. Mol Gen Genet 145: 239–243

    Google Scholar 

  • Donn G (1978) Cell division and callus regeneration from leaf protoplasts of Vicia narbonensis. Z Pflanzenphysiol 86: 65–75

    CAS  Google Scholar 

  • Dorion N, Chupeau Y, Bourgin JP (1975) Isolation, culture and regeneration into plants of Ranunculus sceleratus L. leaf protoplasts. Plant Sci Lett 5: 325–331

    Google Scholar 

  • Dudits K, Kao KN, Constabel F, Gamborg OL (1976) Embryogenesis and formation of tetraploid and hexaploid plants from carrot protoplasts. Can J Bot 5: 1063–1067

    Google Scholar 

  • Durand J, Potrykus I, Donn G (1973) Plantes issues de protoplastes de Petunia. Z Pflanzenphysiol 69: 26–34

    CAS  Google Scholar 

  • Engler DE, Grogan RG (1983) Isolation, culture and regeneration of lettuce leaf mesophyll protoplasts. Plant Sci Lett 28: 223–229

    Google Scholar 

  • Enzmann-Becker G (1973) Plating efficiency of protoplasts of tobacco in different light conditions. Z Naturforsch Sect C Biosci 28: 470–471

    Google Scholar 

  • Eriksson T, Jonasson K (1969) Nuclear division in isolated protoplasts from cells of higher plants grown in vitro. Planta (Berl) 89: 85–89

    Google Scholar 

  • Evans DA (1979) Chromosome stability of plants regenerated from mesophyll protoplasts of Nicotiana species. Z Pflanzenphysiol 95: 459–463

    Google Scholar 

  • Evans PK, Keates AG, Cocking EC (1972) Isolation of protoplasts from cereal leaves. Planta (Berl) 104: 178–181

    CAS  Google Scholar 

  • Evola SV, Earle ED, Chaleff RS (1983) The use of genetic markers selected in vitro for the isolation and genetic verification of intraspecific somatic hybrids of Nicotiana tabacum L. Mol Gen Genet 189: 441–446

    CAS  Google Scholar 

  • Farmer I, Lee PE (1977) Culture of protoplasts derived from Ramsey durum wheat. Plant Sci Lett 10: 141–145

    Google Scholar 

  • Fitter MS, Krikorian AD (1981) Recovery of totipotent cells and plantlet production from day lily protoplasts. Ann Bot (Lond) 48: 591–597

    Google Scholar 

  • Fowke LC, Bech-Hansen CW, Constabel F, Gamborg OL (1974) A comparative study on the ultrastructure of cultured cells and protoplasts of soybean during cell division. Protoplasma 81: 189–203

    Google Scholar 

  • Frearson EM, Power JB, Cocking EC (1973) The isolation, culture and regeneration of Petunia leaf protoplasts. Dev Biol 33: 130–137

    PubMed  CAS  Google Scholar 

  • Fumer IJ, King J, Gamborg OL (1978) Plant regeneration from protoplasts isolated from a pre-dominantly haploid suspension culture of Datura innoxia Mill. Plant Sci Lett 11: 169–176

    Google Scholar 

  • Galbraith DW (1981) Microfluorimetric quantitation of cellulose biosynthesis by plant pro-toplasts using Calcofluor White. Physiol Plant 53: 111–116

    CAS  Google Scholar 

  • Galbraith DW, Shields BA (1982) The effects of inhibitors of cell wall synthesis on tobacco protoplast development. Physiol Plant 55: 25–30

    CAS  Google Scholar 

  • Galston AW, Kaur-Sawhney R, Altman A, Flores H (1980) Polyamines, macromolecular synthesis and the problem of cereal protoplast regeneration. In: Ferenczy L, Farkas GL (eds) Advances in Protoplast Research. Pergamon, Oxford, pp 485–497

    Google Scholar 

  • Galun E (1981) Plant protoplasts as physiological tools. Annu Rev Plant Physiol 32: 237–266

    CAS  Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158

    PubMed  CAS  Google Scholar 

  • Gamborg OL, Miller RA (1973) Isolation, culture and uses of plant protoplasts. Can J Bot 51: 1795–1799

    Google Scholar 

  • Gamborg OL, Constabel F, Fowke L, Kao KN, Ohyama K, Kartha K, Pelcher L (1974) Protoplast and cell culture methods in somatic hybridization in higher plants. Can J Genet Cytol 16: 737–750

    Google Scholar 

  • Gamborg OL, Shyluk JP, Kartha KK (1975) Factors affecting the isolation and callus formation in protoplasts from the shoot apices of Pisum sativum L. Plant Sci Lett 4: 285–292

    CAS  Google Scholar 

  • Gamborg OL, Davis BP, Stahlhut RW (1983) Cell division and differentiation in protoplasts from cell cultures of Glycine species and leaf tissue of soybean. Plant Cell Rep 2: 213–215

    Google Scholar 

  • Gatenby AA, Cocking EC (1977) Callus formation from protoplasts of marrow stem kale. Plant Sci Lett 8: 275–280

    Google Scholar 

  • Gay L (1980) The development of leafy gametophytes from isolated protoplasts of Polytrichum juniperinum Willd. Z Pflanzenphysiol 79: 33–39

    Google Scholar 

  • Gill R, Rashid A, Maheshwari SC (1978) Regeneration of plants from mesophyll protoplasts of Nicotiana plumbaginifolia Viv. Protoplasma 96: 375–379

    Google Scholar 

  • Gill R, Rashid A, Maheshwari SC (1979) Isolation of mesophyll protoplasts of Nicotiana rustica and their regeneration into plants flowering in vitro. Physiol Plant 47: 7–10

    CAS  Google Scholar 

  • Gill R, Rashid A, Maheshwari SC (1981) Dark requirement for cell regeneration and colony formation by mesophyll protoplasts of Nicotiana plumbaginifolia Viv. Protoplasma 106: 351–354

    Google Scholar 

  • Gleba YY (1978) Microdroplet culture: Tobacco plants from single mesophyll protoplasts. Naturwissenschaften 65: 158–159

    Google Scholar 

  • Gosch G, Bajaj YPS, Reinert J (1975 a) Isolation, culture, and fusion studies on protoplasts from different species. Protoplasma 85: 327–336

    Google Scholar 

  • Gosch G, Bajaj YPS, Reinert J (1975 b) Isolation, culture, and induction of embryogenesis in protoplasts from cell-suspensions of Atropa belladonna. Protoplasma 86: 405–410

    Google Scholar 

  • Grambow HJ, Kao KN, Miller RA, Gamborg OL (1972) Cell divisions and plant development from protoplasts of carrot cell suspension cultures. Planta (Berl) 103: 348–355

    Google Scholar 

  • Gresshoff PM (1980) In vitro culture of white clover: callus, suspension, protoplast culture, and plant regeneration. Bot Gaz 141: 157–164

    Google Scholar 

  • Hahne G, Herth W, Hoffmann F (1983) Wall formation and cell division in fluorescence-labelled plant protoplasts. Protoplasma 115: 217–221

    Google Scholar 

  • Halim H, Pearce RS (1980) An electrophoresis method for bulk manipulation of isolated protoplasts from higher plants. Biochem Physiol Pflanz (BPP) 175: 123–129

    CAS  Google Scholar 

  • Hanke DE, Northcote DH (1974) Cell wall formation by soybean callus protoplasts. J Cell Sci 14: 29–50

    PubMed  CAS  Google Scholar 

  • Harms CT, Potrykus I (1978) Enrichment for heterokaryocytes by the use of iso-osmotic density gradients after plant protoplast fusion. Theor Appl Genet 53: 49–55

    Google Scholar 

  • Harms CT, Lörz H, Potrykus I (1979) Multiple-drop-array (MDA) technique for the large-scale testing of culture media variations in hanging microdrop cultures of single cell systems. II. Determination of phytohormone combinations for optimal division response in Nicotiana tabacum protoplast cultures. Plant Sci Lett 14: 237–244

    CAS  Google Scholar 

  • Hasezawa S, Nagata T, Syono K (1981) Transformation of Vinca protoplasts mediated by Agro-bacterium spheroplasts. Mol Gen Genet 182: 206–210

    Google Scholar 

  • Haydu Z, Vasil IK (1981) Somatic embryogenesis and plant regeneration from leaf tissues and anthers of Pennisetum purpureum Schum. Theor Appl Genet 59: 269–273

    Google Scholar 

  • Hayward C, Power JB (1975) Plant production from leaf protoplasts of Petunia parodii. Plant Sci Lett 4: 407–410

    Google Scholar 

  • Heber U (1982) Photosynthesis under osmotic stress: effect of high solute concentrations on the permeability properties of the chloroplast envelope and on activity of stroma enzymes. Planta (Berl) 153: 423–429

    Google Scholar 

  • Herth W, Meyer Y (1977) Ultrastructural and chemical analysis of the wall fibrils synthesized by tobacco mesophyll protoplasts. Biol Cell 30: 33–40

    CAS  Google Scholar 

  • Hess D, Leipoldt G (1979) Regeneration of roots and shoots from isolated mesophyll protoplasts of Nemesia strumosa. Biochem Physiol Pflanz (BPP) 174: 411–417

    Google Scholar 

  • Hooley R (1982) Protoplasts isolated from aleurone layers of wild oat (Avena fatua L.) exhibit the classic response to gibberellic acid. Planta (Berl) 154: 29–40

    CAS  Google Scholar 

  • Hughes BG, White FG, Smith MA (1976) Scanning electron microscopy of barley protoplasts. Protoplasma 90: 399–405

    Google Scholar 

  • Huhtinen O, Honkanen J, Simola LK (1982) Ornithine-and putrescine-supported divisions and cell colony formation in leaf protoplasts of alders (Alnus glutinosa and A. incana). Plant Sci Lett 28: 3–9

    CAS  Google Scholar 

  • Ishii S, Mogi Y (1983) Identification of enzymes that are effective for isolating protoplasts from grass leaves. Plant Physiol (Bethesda) 72: 641–644

    CAS  Google Scholar 

  • Izhar S, Power JB (1977) Genetical studies with Petunia leaf protoplasts. I. Genetic variation to specific growth hormones and possible genetic control on stages of protoplast development in culture. Plant Sci Lett 8: 375–383

    Google Scholar 

  • Jha TB, Roy SC (1979) Rhizogenesis from Nigella sativa protoplasts. Protoplasma 101: 139–142

    Google Scholar 

  • Jia SR (1982) Factors affecting the division frequency of mesophyll protoplasts. Can J Bot 60: 2192–2196

    CAS  Google Scholar 

  • Johnson LB, Stuteville DL, Higgens RK, Douglas HL (1982) Pectolyase Y-23 for isolating mesophyll protoplasts from several Medicago species. Plant Sci Lett 26: 133–137

    Google Scholar 

  • Kaiser G, Heber U (1983) Photosynthesis of leaf cell protoplasts and permeability of plasma-lemma to some solute. Planta (Berl) 157: 462–470

    CAS  Google Scholar 

  • Kameya T (1975) Culture of protoplasts from chimeral plant tissue of nature. Jpn J Genet 50: 417–420

    Google Scholar 

  • Kameya T, Uchimiya H (1972) Embryoids derived from isolated protoplasts of carrot. Planta (Berl) 103: 356–360

    CAS  Google Scholar 

  • Kao KN, Keller WA, Miller RA (1970) Cell division in newly formed cells from protoplasts of soybean. Exp Cell Res 62: 338–340

    PubMed  CAS  Google Scholar 

  • Kao KN, Gamborg OL, Miller RA, Keller WA (1971) Cell divisions in cells regenerated from protoplasts of soybean and Haplopappus gracilis. Nature New Biol 232: 124

    Google Scholar 

  • Kao KN, Gamborg OL, Michayluk MR, Keller WA, Miller RA (1973) The effects of sugars and inorganic salts on cell regeneration and sustained division in plant protoplasts. In: Protoplastes et fusion de cellules somatiques vegetales. Colloq Int Cent Natl Rech Sci 212: 207–213

    Google Scholar 

  • Kao KN, Michayluk MR (1975) Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta (Berl) 126: 105–110

    CAS  Google Scholar 

  • Kao KN, Michayluk MR (1980) Plant regeneration from mesophyll protoplasts of alfalfa. Z Pflanzenphysiol 96: 135–141

    Google Scholar 

  • Kartha KK, Michayluk MR, Kao KN, Gamborg OL, Constabel F (1974) Callus formation and plant regeneration form mesophyll protoplasts of rape plants (Brassica napus L. cv. Zephyr ). Plant Sci Lett 3: 265–271

    Google Scholar 

  • Kaur-Sawhney R, Flores HE, Galston AW (1980) Polyamine-induced DNA synthesis and mitosis in oat leaf protoplasts. Plant Physiol (Bethesda) 65: 368–371

    CAS  Google Scholar 

  • Kim IS, Song PS (1981) Binding of phytochrome to liposomes and protoplasts. Biochemistry 20: 5482–5489

    PubMed  CAS  Google Scholar 

  • Kinnersley AM, Racusen RH, Galston AW (1978) A comparison of regenerated cell walls in tobacco and cereal protoplasts. Planta (Berl) 139: 155–158

    Google Scholar 

  • Kirby EG, Cheng T-Y (1979) Colony formation from protoplasts derived from Douglas fir cotyledons. Plant Sci Lett 14: 145–154

    CAS  Google Scholar 

  • Klein AS, Montezinos D, Delmer DP (1981) Cellulose and 1,3-glucan synthesis during the early stages of wall regeneration in soybean protoplasts. Planta (Berl) 152: 105–114

    CAS  Google Scholar 

  • Klercker I (1892) Eine Methode zur Isolierung lebender Protoplasten. Öfvers Vet-Akad Förhdl 9: 463–474

    Google Scholar 

  • Koblitz H (1976) Isolierung und Kultivierung von Protoplasten aus Calluskulturen der Gerste. Biochem Physiol Pflanz (BPP) 170: 287–293

    Google Scholar 

  • Kohlenbach HW, Bohnke E (1975) Isolation and culture of mesophyll protoplasts of Hyoscyamus piger L. var. annuus Sims. Experientia (Basel) 31: 1281–1282

    Google Scholar 

  • Kohlenbach HW, Wenzel C, Hoffmann F (1982) Regeneration of Brassica napus plantlets in cultures from isolated protoplasts of haploid stem embryos as compared with leaf protoplasts. Z Pflanzenphysiol 105: 131–142

    Google Scholar 

  • Koop H-U, Weber G, Schweiger H-G (1983) Individual culture of selected single cells and protoplasts of higher plants in microdroplets of defined media. Z Pflanzenphysiol 112: 21–34

    Google Scholar 

  • Kowalczyk TP, Mackenzie IA, Cocking EC (1983) Plant regeneration from organ explants and protoplasts of the medicinal plant Solanum khasianum CB Clarke var. chatterjeeanum Sengupta (Syn. Solanum viarum Dunal). Z Pflanzenphysiol 111: 55–68

    CAS  Google Scholar 

  • Krikorian AD (1982) Cloning higher plants from aseptically cultured tissues and cells. Biol Rev Camb Philos Soc 57: 151–218

    Google Scholar 

  • Lloyd CW, Slabas AR, Powell AJ, Lowe SB (1980) Microtubules, protoplasts and plant cell shape, an immunofluorescent study. Planta (Berl) 147: 500–506

    Google Scholar 

  • Lörz H, Potrykus I, Thomas E (1977) Somatic embryogenesis from tobacco protoplasts. Naturwissenschaften 64: 439–440

    Google Scholar 

  • Lörz H, Wernicke W, Potrykus I (1979) Culture and plant regeneration of Hyoscyamus protoplasts. Planta Med 36: 21–29

    PubMed  Google Scholar 

  • Lu CY, Vasil IK (1981) Somatic embryogenesis and plant regeneration in tissue cultures of Panicum maximum Jacq. Am J Bot 69: 77–81

    Google Scholar 

  • Lu CY, Vasil V, Vasil IK (1981) Isolation and culture of protoplasts of Panicum maximum Jacq. (Guinea grass): somatic embryogenesis and plantlet formation. Z Pflanzenphysiol 104: 311–318

    Google Scholar 

  • Lu DY, Cooper-Bland S, Pental D, Cocking EC, Davey MC (1983) Isolation and sustained division of protoplasts from cotyledons of seedlings and immature seeds of Glycine max L. Z Pflanzenphysiol 111: 389–394

    CAS  Google Scholar 

  • Lu DY, Davey MR, Cocking EC (1983) A comparison of the cultural behaviour of protoplasts from leaves, cotyledons and roots of Medicago sativa. Plant Sci Lett 31: 87–89

    Google Scholar 

  • Mackenzie IA, Bui-Dang-Ha D, Davey MR (1973) Some aspects of the isolation, fine structure and growth of protoplasts from Asparagus officinalis L. In: Protoplastes et fusion de cellules somatiques vegetales. Colloq Int Cent Natl Rech Sci 212: 291–299

    Google Scholar 

  • Maheshwari SC, Rashid A, Tyagi AK (1982) Haploids from pollen grains — retrospect and prospect. Am J Bot 69: 865–879

    Google Scholar 

  • Meijer EGM, Steinbiss H-H (1983) Plantlet regeneration from suspension and protoplast cultures of the tropical pasture legume Stylosanthes guyanensis ( Aubl.) Sw. Ann Bot (Lond) 52: 305–310

    Google Scholar 

  • Menczel L, Lazar C, Maliga P (1978) Isolation of somatic hybrids by cloning Nicotiana heterokaryons in nurse cultures. Planta (Berl) 143: 29–32

    Google Scholar 

  • Messerschmidt M (1974) Callus formation and differentiation of isolated protoplasts from cotyledons of Pharbitis nil. Z Pflanzenphysiol 74: 175–178

    Google Scholar 

  • Meyer Y (1974) Isolation and culture of tobacco mesophyll protoplasts using a saline medium. Protoplasma 81: 363–372

    Google Scholar 

  • Meyer Y, Abel WO (1975) Budding and cleavage division of tobacco mesophyll protoplasts in relation to pseudo-wall and wall formation. Planta (Berl) 125: 1–13

    CAS  Google Scholar 

  • Meyer Y, Cooke R (1979) Time course of hormonal control of the first mitosis in tobacco mesophyll protoplasts cultivated in vitro. Planta (Berl) 147: 181–185

    CAS  Google Scholar 

  • Meyer Y, Herth W (1978) Chemical inhibition of cell wall formation and cytokinesis, but not of nuclear division in protoplasts of Nicotiana tabacum L. cultivated in vitro. Planta (Berl) 142: 253–262

    CAS  Google Scholar 

  • Michayluk MR, Kao KN (1975) A comparative study of sugars and sugar alcohols on cell re-generation and sustained cell division in plant protoplasts. Z Pflanzenphysiol 75: 181–185

    CAS  Google Scholar 

  • Morgan A, Cocking EC (1982) Plant regeneration from protoplasts of Lycopersicon esculentum Mill. Z Pflanzenphysiol 106: 97–104

    Google Scholar 

  • Mühlbach HP (1980) Different regeneration potentials of mesophyll protoplasts from cultivated and a wild species of tomato. Planta (Berl) 148: 89–96

    Google Scholar 

  • Mühlbach HP, Thiele H (1981) Response to chilling of tomato mesophyll protoplasts. Planta (Berl) 151: 399–401

    Google Scholar 

  • Muller J F, Missionier C, Caboche M (1983) Low density growth of cells derived from Nicotiana and Petunia protoplasts: influence of the source of protoplasts and comparison of growth-promoting activity of various auxins. Physiol Plant 57: 35–41

    CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15: 473–497

    CAS  Google Scholar 

  • Nagata T, Ishii S (1979) A rapid method for isolation of mesophyll protoplasts. Can J Bot 57: 1820–1823

    Google Scholar 

  • Nagata T, Takebe I (1970) Cell wall regeneration and cell division in isolated tobacco mesophyll protoplasts. Planta (Berl) 92: 301–308

    Google Scholar 

  • Nagata T, Takebe I (1971) Plating of isolated tobacco mesophyll protoplasts on agar medium. Planta (Berl) 99: 12–20

    Google Scholar 

  • Nagy JI, Maliga P (1976) Callus induction and plant regeneration from mesophyll protoplasts of Nicotiana sylvestris. Z Pflanzenphysiol 78: 453–455

    Google Scholar 

  • Nebiolo CM, Kaczmarczyk WJ, Ulrich V (1983) Manifestation of hybrid vigor in RNA synthesis parameters by corn seedling protoplasts in the presence and absence of gibberellic acid. Plant Sci Lett 28: 195–206

    CAS  Google Scholar 

  • Negrutiu I, Mousseau J (1980) Protoplast culture from in vitro grown plants of Nicotiana sylves-tris Spegg. and Comes. Z Pflanzenphysiol 100: 373–376

    Google Scholar 

  • Nehls R (1978) Isolation and regeneration of protoplasts from Solanum nigrum L. Plant Sci Lett 12: 183–187

    CAS  Google Scholar 

  • Nelson RS, Creissem GP, Bright SWJ (1983) Plant regeneration from protoplasts of Solanum brevidens. Plant Sci Lett 30: 355–362

    CAS  Google Scholar 

  • Nemet G, Dudits D (1977) Potentials of protoplasts, cell and tissue culture in cereal research. In: Novak FJ (ed) Use of tissue culture in plant breeding. Czechoslovak Acad Sci, Prague, pp 145–163

    Google Scholar 

  • Norman HA, Black M, Chapman JM (1983) The induction of sensitivity to gibberellin in aleurone tissue of developing wheat grain: sensitization of isolated protoplasts. Planta (Berl) 158: 264–271

    CAS  Google Scholar 

  • Oelck MM, Bapat VA, Schieder O (1982) Protoplast culture of three legumes: Arachis hypogaea, Melilotus officinalis, Trifolium resupinatum. Z Pflanzenphysiol 106: 173–177

    Google Scholar 

  • Ohyama K, Nitsch JP (1972) Flowering haploid plants obtained from protoplasts of tobacco leaves. Plant Cell Physiol 13: 229–236

    Google Scholar 

  • Ono K, Ohyama K, Gamborg OL (1979) Regeneration of the liverwort Marchantia polymorpha L. from protoplasts isolated from cell suspension culture. Plant Sci Lett 14: 225–229

    Google Scholar 

  • Passiatore JE, Sink KC (1981) Plant regeneration from leaf mesophyll protoplasts of selected or-namental Nicotiana species. J Soc Hortic Sci 106: 779–803

    Google Scholar 

  • Patnaik G, Wilson D, Cocking EC (1981) Importance of enzyme purification of increased plating efficiency and plant regeneration from single protoplasts of Petunia parodii. Z Pflanzenphysiol 102: 199–205

    CAS  Google Scholar 

  • Pelcher LE, Gamborg OL, Kao KN (1974) Bean mesophyll protoplasts: production, culture and callus formation. Plant Sci Lett 3: 107–111

    Google Scholar 

  • Poirier-Hamon S, Rao PS, Harada H (1974) Culture of mesophyll protoplasts and stem segments of Antirrhinum magus ( Snapdragon ): Growth and organization of embryoids. J Exp Bot 25: 752–760

    Google Scholar 

  • Potrykus I (1980) The old problem of protoplast culture: cereals. In: Ferenczy L, Farkas GL (eds) Advances in Protoplast Research. Pergamon, Oxford, pp 243–254

    Google Scholar 

  • Potrykus I, Durand J (1972) Callus formation from single protoplasts of Petunia. Nature New Biol 237: 286–287

    PubMed  CAS  Google Scholar 

  • Potrykus I, Harms CT, Lörz H (1976) Problems in culturing cereal protoplasts. In: Dudits D, Farkas GL, Maliga P (eds) Cell Genetics in Higher Plants. Publishing House of the Hungarian Academy of Sciences, Budapest, pp 129–140

    Google Scholar 

  • Potrykus I, Harms CT, Lörz H, Thomas E (1977) Callus formation from stem protoplasts of corn (Zea mays L.) Mol Gen Genet 156: 347–350

    CAS  Google Scholar 

  • Potrykus I, Harms CT, Lörz H (1979 a) Callus formation from cell culture protoplasts of corn (Zea mays L.). Theor Appl Genet 54: 209–214

    Google Scholar 

  • Potrykus I, Harms CT, Lörz H ( 1979 b) Multiple-drop-array (MDA) technique for the large-scale testing of culture media variations in hanging microdrop cultures of single cell systems. I. The technique. Plant Sci Lett 14: 231–235

    Google Scholar 

  • Power JB, Berry SF (1979) Plant regeneration from protoplasts of Browallia viscosa. Z Pflanzenphysiol 94: 469–471

    Google Scholar 

  • Power JB, Frearson EM, George D, Evans PK, Berry SF, Hayward C, Cocking EC (1976) The isolation, culture and regeneration of leaf protoplasts in the genus Petunia. Plant Sci Lett 7: 1–55

    Google Scholar 

  • Racusen RH, Kinnersley AM, Galston AW (1977) Osmotically induced changes in electrical properties of plant protoplast membranes. Science (Wash DC) 198: 405–407

    CAS  Google Scholar 

  • Rahat M, Reinhold L (1983) Rb uptake by isolated pea mesophyll protoplasts in light and darkness. Physiol Plant 59: 83–90

    CAS  Google Scholar 

  • Rao PS (1982) Protoplast culture. In: Johri BM (ed) Experimental Embryology of Vascular Plants. Springer, Berlin Heidelberg New York, pp 231–262

    Google Scholar 

  • Raveh D, Galun E (1975) Rapid regeneration of plants from tobacco protoplasts plated at low densities. Z Pflanzenphysiol 76: 76–79

    Google Scholar 

  • Raveh D, Huberman E, Galun E (1973) In vitro culture of tobacco protoplasts: use of feeder techniques to support division of cells plated at low densities. In Vitro (Rockville) 9: 216222

    Google Scholar 

  • Roscoe DH, Bell GM (1981) Use of a pH indicator in protoplast culture medium. Plant Sci Lett 21: 275–279

    CAS  Google Scholar 

  • Ruesink AW, Thimann KV (1965) Protoplasts from the Avena coleoptile. Proc Natl Acad Sci USA 54: 56–64

    PubMed  CAS  Google Scholar 

  • Santos AVF Dos, Outka DE, Cocking EC, Davey MR (1980) Organogenesis and somatic embryogenesis in tissues derived from leaf protoplasts and leaf explants of Medicago sativa. Z Pflanzenphysiol 99: 261–270

    Google Scholar 

  • Saxena PK, Rashid A (1980) Development of gametophores from isolated protoplasts of the moss Anoectangium thomsonii Mitt. Protoplasma 103: 401–404

    Google Scholar 

  • Saxena PK, Rashid A (1981) High frequency regeneration of Funaria hygrometrica protoplasts isolated from low calcium protonemal suspension. Plant Sci Lett 23: 117–122

    CAS  Google Scholar 

  • Saxena PK, Gill R, Rashid A, Maheshwari SC (1981 a) Plantlet formation from isolated pro-toplasts of Solanum melongena L. Protoplasma 106: 355–359

    Google Scholar 

  • Saxena PK, Gill R, Rashid A, Maheshwari SC (1981 b) Isolation and culture of protoplasts of Capsicum annuum L. and their regeneration into plants flowering in vitro. Protoplasma 108: 357–360

    Google Scholar 

  • Saxena PK, Gill R, Rashid A, Maheshwari SC (1982 a) Colony formation by cotyledonary protoplasts of Cyamopsis tetragonoloba L. Z Pflanzenphysiol 106: 277–280

    Google Scholar 

  • Saxena PK, Gill R, Rashid A, Maheshwari SC (1982b) Plantlets from mesophyll protoplasts of Solanum xanthocarpum. Plant Cell Rep 1: 219–220

    Google Scholar 

  • Schenk HR, Hoffmann F (1979) Callus and root regeneration from mesophyll protoplasts of basic Brassica species: Brassica campestris, B. oleracea and B. nigra. Z Pflanzenzuecht 82: 354–360

    Google Scholar 

  • Schieder 0 (1975) Regeneration von haploiden and diploiden Datura innoxia Mill. Mesophyll Protoplasten zu Pflanzen. Z Pflanzenphysiol 76: 462–466

    Google Scholar 

  • Schieder O (1977) Attempts in regeneration of mesophyll protoplasts of haploid and diploid wild type lines, and those of chlorophyll-deficient strains from different Solanaceae. Z Pflanzenphysiol 84: 275–281

    Google Scholar 

  • Schilde-Rentschler L (1977) Role of the cell wall in the ability of tobacco protoplasts to form callus. Planta (Berl) 135: 177–181

    CAS  Google Scholar 

  • Schumann U, Opatrny Z, Koblitz H (1980) Plant recovery from long term callus cultures and from suspension culture derived protoplasts of Solanum phureja. Biochem Physiol Pflanz (BPP) 175: 670–675

    Google Scholar 

  • Scowcroft WR, Larkin PJ (1980) Isolation, culture and plant regeneration from protoplasts of Nicotiana debneyi. Aust J Plant Physiol 7: 635–644

    CAS  Google Scholar 

  • Shahin EA, Shepard JF (1980) Cassava mesophyll protoplasts: Isolation, proliferation, and shoot formation. Plant Sci Lett 17: 459–465

    Google Scholar 

  • Shakurov MI (1982) Cultivation of isolated protoplasts of different species of the genus Nicotiana. Soy Plant Physiol (Engl Transl Fiziol Rast) 29: 132–140

    Google Scholar 

  • Shekhawat NS, Galston AW (1983 a) Mesophyll protoplasts of fenugreek (Trigonella foenum-graecum): isolation, culture and shoot regeneration. Plant Cell Rep 2: 119–121

    Google Scholar 

  • Shekhawat NS, Galston AW (1983 b) Isolation, culture and regeneration of mothbean Vigna aconitifolia leaf protoplasts. Plant Sci Lett 32: 43–51

    Google Scholar 

  • Shepard JF (1980) Mutant selection and plant regeneration from potato mesophyll protoplasts. In: Rubenstein I, Gengenbach B, Phillips RL, Green CE (eds) Emergent techniques for the genetic improvement of crops. Univ Minnesota Press, Minneapolis, pp 185–219

    Google Scholar 

  • Shepard JF, Totten RE (1975) Isolation and regeneration of tobacco mesophyll cell protoplasts under low osmotic conditions. Plant Physiol (Bethesda) 55: 689–694

    CAS  Google Scholar 

  • Shepard JF, Totten RE (1977) Mesophyll cell protoplasts of potato — isolation, proliferation, and plant regeneration. Plant Physiol (Bethesda) 60: 313–316

    CAS  Google Scholar 

  • Shillito RD, Paszkowski J, Potrykus I (1983) Agarose plating and a bead type culture technique enable and stimulate development of protoplast-derived colonies in a number of plant species. Plant Cell Rep 2: 244–247

    CAS  Google Scholar 

  • Sidorov V, Menczel L, Maliga P (1981) Isoleucine-requiring Nicotiana plant deficient in threonine deaminase. Nature (Lond) 294: 87–88

    CAS  Google Scholar 

  • Simmonds JA, Simmonds DH, Cumming BG (1979) Isolation and cultivation of protoplasts from morphogenetic callus cultures of Lilium. Can J Bot 57: 512–516

    CAS  Google Scholar 

  • Sink KC, Power JB (1977) The isolation, culture and regeneration of leaf protoplasts of Petunia parviflora Juss. Plant Sci Lett 10: 335–340

    Google Scholar 

  • Steward FC, Krikorian AD (1979) Problems and potentialities of cultured plant cells in retrospect and prospect. In: Sharp WR, Larsen PO, Paddock EF, Raghavan V (eds) Plant cell tissue culture, principles and applications. Ohio State Univ Press, Columbus, pp 221–262

    Google Scholar 

  • Stumm I, Meyer Y, Abel WO (1975) Regeneration of the moss Physcomitrella patens Hedw. from isolated protoplasts. Plant Sci Lett 5: 113–118

    Google Scholar 

  • Takebe I, Otsuki Y (1973) Fine structure of isolated mesophyll protoplasts of tobacco. Planta (Berl) 113: 21–27

    Google Scholar 

  • Takebe I, Labib G, Melchers G (1971) Regeneration of whole plants from isolated mesophyll protoplasts of tobacco. Naturwissenschaften 58: 318–320

    Google Scholar 

  • Takeuchi Y, Komamine A (1978) Composition of the cell wall formed by protoplasts isolated from cell suspension cultures of Vinca rosea. Planta (Berl) 140: 227–232

    CAS  Google Scholar 

  • Takeuchi Y, Komamine A (1982) Effects of culture conditions on cell division and composition of regenerated cell walls in Vinca rosea protoplasts. Plant Cell Physiol 23: 249–255

    CAS  Google Scholar 

  • Thomas E, Hoffmann F, Potrykus I, Wenzel G (1976) Protoplast regeneration and stem embryo-genesis of haploid androgenetic rape. Mol Gen Genet 145: 245–247

    Google Scholar 

  • Thomas E, King PJ, Potrykus I (1979) Improvement of crop plants via single cells in vitro — an assessment. Z Pflanzenzuecht 82: 1–30

    Google Scholar 

  • Thomas E, Brettell R, Wernicke W (1980) Problems in plant regeneration from protoplasts of important crops. In: Ferenczy L, Farkas GL (eds) Advances in protoplasts research. Perga-mon, Oxford, pp 269–274

    Google Scholar 

  • Thomas E (1981) Plant regeneration from shoot culture-derived protoplasts of tetraploid potato (Solanum tuberosum cv. Maris Bard). Plant Sci Lett 23: 81–88

    Google Scholar 

  • Thomas E, Bright SWJ, Franklin J, Lancaster VA, Miflin MJ, Gibson R (1982) Variation amongst protoplast-derived potato plants (Solanum tuberosum cv. Maris Bard ). Theor Appl Genet 72: 65–68

    Google Scholar 

  • Uchimiya H, Murashige T (1976) Influence of the nutrient medium on the recovery of dividing cells from tobacco protoplasts. Plant Physiol (Bethesda) 57: 424–429

    CAS  Google Scholar 

  • Ulrich TH, Chowdhury JB, Widholm JM (1980) Callus and root formation from mesophyll protoplasts of Brassica rapa. Plant Sci Lett 19: 347–354

    CAS  Google Scholar 

  • Upadhya MD (1975) Isolation and culture of mesophyll protoplasts of potato (Solanum tuberosum L.). Potato Res 18: 438–445

    Google Scholar 

  • Vardi A, Spiegel-Roy P, Galun E (1975) Citrus cell culture: isolation of protoplasts, plating densities, effect of mutagens and regeneration of embryos. Plant Sci Lett 4: 231–236

    Google Scholar 

  • Vardi A, Spiegel-Roy P, Galun E (1982) Plant regeneration from Citrus protoplasts: variability in methodological requirements among cultivars and species. Theor Appl Genet 62: 171–176

    Google Scholar 

  • Vasil IK (1982) Plant cell culture and somatic cell genetics of cereals and grasses. In: Vasil IK, Scowcroft WR, Frey KJ (eds) Plant improvement and somatic cell genetics. Academic, New York, pp 179–203

    Google Scholar 

  • Vasil IK (ed) (1984) Cell culture and somatic cell genetics of plants. Vol 1. Laboratory procedures and their applications. Academic, New York

    Google Scholar 

  • Vasil IK, Vasil V (1980) Isolation and culture of protoplasts. Int Rev Cytol Suppl 11B: 1–19

    Google Scholar 

  • Vasil V, Vasil IK (1974) Regeneration of tobacco and Petunia plants from protoplasts and culture of corn protoplasts. In Vitro (Rockville) 10: 83–96

    Google Scholar 

  • Vasil V, Vasil IK (1979) Isolation and culture of cereal protoplasts. I. Callus formation from pearl millet (Pennisetum americanum) protoplasts. Z Pflanzenphysiol 92: 379–384

    Google Scholar 

  • Vasil V, Vasil IK (1980) Isolation and culture of cereal protoplasts. II. Embryogenesis and plant- let formation from protoplasts of Pennisetum americanum. Theor Appl Genet 56: 97–99

    Google Scholar 

  • Vasil V, Wang D-Y, Vasil IK (1983) Plant regeneration from protoplasts of napier grass (Pen-nisetum purpureum Schum.). Z Pflanzenphysiol 111: 233–239

    CAS  Google Scholar 

  • Wallin A, Eriksson T (1973) Protoplast culture from cell suspensions of Daucus carota. Physiol Plant 28: 33–39

    Google Scholar 

  • Wenzel G (1973) Isolation of leaf protoplasts from haploid plants of Petunia, rape and rye. Z Pflanzenzuecht 69: 58–61

    Google Scholar 

  • Wenzel G, Schieder 0 (1973) Regeneration of isolated protoplasts from nicotinic acid-deficient mutants of the liverwort Sphaerocarpos donnellii Aust. Plant Sci Lett 1: 421–423

    CAS  Google Scholar 

  • Wernicke W, Brettell RIS (1982) Morphogenesis from cultured leaf tissue of Sorghum bicolor —culture initiation. Protoplasma 111: 19–27

    CAS  Google Scholar 

  • Wernicke W, Thomas E (1980) Studies on morphogenesis from isolated plant protoplasts: shoot formation from mesophyll protoplast of Hyoscyamus muticus and Nicotiana tabacum. Plant Sci Lett 17: 401–407

    CAS  Google Scholar 

  • Wernicke W, Lörz H, Thomas E (1979) Plant regeneration from leaf protoplasts of haploid Hyoscyamus muticus L. produced via anther culture. Plant Sci Lett 15: 239–249

    Google Scholar 

  • Willison JHM, Cocking EC (1975) Microfibril synthesis at the surface of isolated tobacco mesophyll protoplasts, a freeze-etch study. Protoplasma 84: 147–159

    Google Scholar 

  • Willison JHM, Grout BWW (1978) Further observations on cell-wall formation around isolated protoplasts of tobacco and tomato. Planta (Berl) 140: 53–58

    Google Scholar 

  • Xiang-hui Li (1981) Plantlet regeneration from mesophyll protoplasts of Digitalis lanata Ehrh. Theor Appl Genet 60: 345–347

    Google Scholar 

  • Xiang-hui Li, Yan Qui-sheng, Huang Mei-juan, Sun Yung-ru, Li Wen-bin (1980) Division of cells regenerated from mesophyll protoplasts of wheat (Triticum aestivum L.) In: Ferenczy L, Farkas GL (eds) Advances in protoplast research. Pergamon, Oxford, pp 261–267

    Google Scholar 

  • Xu X-H, Davey MR (1983) Shoot regeneration from mesophyll protoplasts and leaf explants of Rehmannia glutinosa. Plant Cell Rep 2: 55–57

    Google Scholar 

  • Xu Z-H, Davey MR, Cocking EC (1981) Isolation and sustained division of Phaseolus aureus (mung bean) root protoplasts. Z Pflanzenphysiol 104: 289–298

    Google Scholar 

  • Xu Z-H, Davey MR, Cocking EC (1982) Plant regeneration from root protoplasts of Brassica. Plant Sci Lett 24: 117–121

    Google Scholar 

  • Xuan LT, Menczel L (1980) Improved protoplast culture and plant regeneration from protoplast-derived callus in Arabidopsis thaliana. Z Pflanzenphysiol 96: 77–80

    Google Scholar 

  • Zamura AB, Scott KI (1983) Callus formation and plant regeneration from wheat leaves. Plant Sci Lett 29: 183–189

    Google Scholar 

  • Zapata FJ, Sink KC (1981) Somatic embryogenesis from Lycopersicon peruvianum leaf mesophyll protoplasts. Theor Appl Genet 59: 265–268

    Google Scholar 

  • Zapata FJ, Evans PK, Power JB, Cocking EC (1977) The effect of temperature on the division of leaf protoplasts of Lycopersicon esculentum and Lycopersicon peruvianum. Plant Sci Lett 8: 119–124

    Google Scholar 

  • Zapata FJ, Sink KC, Cocking EC (1981) Callus formation from leaf mesophyll protoplasts of three Lycopersicon species: L. esculentum cv. Walter, L. pimpinillifolium and L. hirsutum f. glabratum. Plant Sci Lett 23: 41–46

    CAS  Google Scholar 

  • Arcioni S, Mariotti D, Pezotti M (1985) Hedysarum coronarium L. In vitro conditions for plant regneration from protoplasts and callus of various explants. J Plant Physiol 121: 141–148

    Google Scholar 

  • Bass A, Hughes W (1984) Conditions for isolation and regeneration of viable protoplasts of oil palm (Elaeis guineensis). Plant Cell Rep 3: 169–171

    Google Scholar 

  • Bright SWJ, Jones MGK (eds) (1985) Cereal Tissue and Cell Culture. Nijhoff/Junk

    Google Scholar 

  • Chatterjee G, Sikdar SR, Das S, Sen SK (1985) Regeneration of plantlets from mesophyll protoplasts of Brassica juncea ( L) Czern. Plant Cell Rep 4: 245–247

    Google Scholar 

  • Chourey PS, Sharpe DZ (1985) Callus from protoplasts of Sorghum cell suspension cultures. Plant Sci 39: 171–175

    Google Scholar 

  • Giles KL (ed) (1983) Plant Protoplasts. Int Rev Cytol Suppl 16

    Google Scholar 

  • Gleddie S, Keller WA, Setterfield G (1985) Plant regeneration from tissue, cell and protoplast cultures of several wild Solanum species. J Plant Physiol 119: 405–418

    CAS  Google Scholar 

  • Glimelius K (1984) High growth rate and regeneration capacity of hypocotyl protoplasts in some Brassicaceae. Physiol Plant 61: 38–44

    CAS  Google Scholar 

  • Gorton HL, Satter RL (1984) Extensor and flexor protoplasts from Samanea pulvini. II. X-ray analysis of potassium, chlorine, sulfur, phosphorus and calcium. Plant Physiol (Bethesda) 76: 685–690

    Google Scholar 

  • Griffing LR, Cutler AJ, Shargool PD, Fowke LC (1985) Isoelectric focussing of plant cell protoplasts. Plant Physiol (Bethesda) 77: 765–769

    CAS  Google Scholar 

  • Grosser JW, Collins GB (1985) Isolation and culture of Trifolium rubens protoplasts with whole plant regeneration. Plant Sci Lett 37: 165–170

    Google Scholar 

  • Hassanpour-Estahbanati A, Demarly Y (1985) Plant regeneration from protplasts of Solanum pennellii: Effect of photoperiod applied to donor plant. J Plant Physiol 121: 171–174

    Google Scholar 

  • Heyser JW (1984) Callus and shoot regeneration from protoplasts of Proso millet (Panicum mil-iaceum L.). Z Pflanzenphysiol 113: 293–299

    CAS  Google Scholar 

  • Koop H-U, Schweiger H-G (1985) Regeneration of plants from individually cultivated pro-toplasts using an improved microculture system. J Plant Physiol 121: 245–257

    Google Scholar 

  • Li GG, Constabel F (1984) Plant regeneration with callus and protoplasts of Solanum uporo Dun. J Plant Physiol 117: 137–142

    PubMed  CAS  Google Scholar 

  • Mariotti D, Arcioni S, Pezzotti M (1984) Regeneration of Medicago arborea L. plants from tissue and protoplast cultures of different organ origin. Plant Sci Lett 37: 149–156

    Google Scholar 

  • Mhatre M, Bapat VA, Rao PS (1984) Plant regeneration in protoplast cultures of Tylophora indica. J Plant Physiol 115: 231–235

    PubMed  CAS  Google Scholar 

  • Moran N, Ehrenstein G, Iwasa K, Bare C, Mischke C (1984) Ion channels in plasmalemma of wheat protoplasts. Science (Wash DC) 226: 835–837

    CAS  Google Scholar 

  • Negrutiu I, Brouwer D De, Dirks R, Jacobs M (1985) Amino acid auxotrophs from protoplasts cultures of Nicotiana plumbaginifolia Viviani I. BUdR enrichment selection, plant regeneration and general characterisation. Mol Gen Genet 199: 330–337

    Google Scholar 

  • Negrutiu I, Jacobs M, Caboche M (1984) Advances in somatic cell genetics of higher plants — the protoplast approach in basic studies on mutagenesis and isolation of biochemical mutants. Theor Appl Genet 67: 289–304

    Google Scholar 

  • Newell CA, Luu HT (1985) Protoplast culture and plant regeneration in Glycine canescens FJ Herm. Plant Cell Tissue Organ Culture 4: 145–149

    Google Scholar 

  • Oka S, Ohyama K (1985) Plant regeneration from leaf mesophyll protoplasts of Broussonetia kazinoki Sieb ( Paper mulberry ). J Plant Physiol 119: 455–460

    Google Scholar 

  • Orzyk W, Malepszy S (1985) In vitro culture of Cucumis sativus L. V. Stabilizing effect of glycine on leaf protplasts. Plant Cell Rep 4: 269–273

    Google Scholar 

  • Potrykus I, Harms CT, Hinnen A, Hutter R, King PJ, Shillito RD (eds) (1983) Proc 6th Int Protoplast Symp, Basel

    Google Scholar 

  • Rao PS, Ozias-Akins P (1985) Plant regeneration through somatic embryogenesis in protoplast cultures of sandalwood, (Santalum album L.) Protoplasma 124: 80–86

    Google Scholar 

  • Shneyour Y, Zelcer A, Shamay I, Beckmann JA (1984) A simple feeder layer technique for the plating of plant cells and protoplasts at low density. Plant Sci Lett 33: 293–302

    Google Scholar 

  • Swanson EB, Wong RSC, Kemble RJ (1985) A novel method for the isolation and purificaton of protoplasts from friable, embryogenic corn (Zea mays L.) callus. Plant Sci 40: 137–144

    Google Scholar 

  • Toriyama K, Hinata K (1985) Cell suspension and protoplast culture in rice. Plant Sci 491: 179–183

    Google Scholar 

  • Vasil IK (ed) (1984) Cell culture and somatic cell genetics of plants Vol 1. Academic Press, London Toronto Tokyo

    Google Scholar 

  • Wilson VM, Haq M, Evans PK (1985) Protoplast isolation, culture and plant regeneration in the winged bean, Psophocarpus tetragonolobus ( L) DC. Plant Sci 41: 61–68

    Google Scholar 

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Authors and Affiliations

  1. Unit for Plant Cell and Molecular Biology, University of Delhi, 110007, Delhi, India

    S. C. Maheshwari, N. Maheshwari & P. K. Gharyal

  2. Bio-Organic Division, Bhabha Atomic Research Centre, Bombay, India

    R. Gill

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  1. Institut für Pflanzenphysiologie, Königin-Luise-Straße 12-16, D-1000, Berlin 33, Germany

    J. Reinert

  2. Botanisches Institut der Universität, Olshausenstraße 40-60, D-2300, Kiel 1, Germany

    H. Binding

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© 1986 Springer-Verlag Berlin Heidelberg

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Maheshwari, S.C., Gill, R., Maheshwari, N., Gharyal, P.K. (1986). Isolation and Regeneration of Protoplasts from Higher Plants. In: Reinert, J., Binding, H. (eds) Differentiation of Protoplasts and of Transformed Plant Cells. Results and Problems in Cell Differentiation, vol 12. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-39836-3_2

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  • DOI: https://doi.org/10.1007/978-3-540-39836-3_2

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-21816-7

  • Online ISBN: 978-3-540-39836-3

  • eBook Packages: Springer Book Archive

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