The signal from galactose is transmitted to Gal80p-Gal4p transcriptional switch through Gal3p/Gal1p signaling system. Based on the genetic experiments discussed previously, it appeared unlikely that signaling is due to a derivative of galactose catalyzed by Gal3p. Attempts to dissociate Gal4p-Gal80p complex in presence of galactose derivatives were unsuccessful, but it was clear that at least galactokinase activity is not required for signaling. Therefore, an idea that necessitates the presence of galactose for induction but does not warrant that the signal transducer to be an enzyme was considered.
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(2008). Signal Transduction Revisited. In: Galactose Regulon of Yeast. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-74015-5_6
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