Abstract
The reliable and fast detection of chemical or biological molecules, or the measurement of their concentrations in a sample, are key problems in many fields such as environmental analysis, medical diagnosis, or the food industry. There are traditionally two approaches to this problem. The first aims to carry out a measurement in situ in the sample using chemical and biological sensors. The constraints imposed by detection limits, specificity, and in some cases stability are entirely imputed to the sensor. The second approach uses so-called total analysis systems to process the sample according to a protocol made up of different steps, such as extractions, purifications, concentrations, and a final detection stage. The latter is made in better conditions than with the first approach, which may justify the greater complexity of the process. It is this approach that is implemented in most methods for identifying pathogens, whether they be in biological samples (especially for in vitro diagnosis) or samples taken from the environment. The instrumentation traditionally used to carry out these protocols comprises a set of bulky benchtop apparatus, which needs to be plugged into the mains in order to function. However, there are many specific applications (to be discussed in this chapter) for which analysis instruments with the following characteristics are needed: Possibility of use outside the laboratory, i.e., instruments as small as possible, consuming little energy, and largely insensitive to external conditions of temperature, humidity, vibrations, and so on. Possibility of use by non-specialised agents, or even unmanned operation. Possibility of handling a large number of samples in a limited time, typically for high-throughput screening applications. Possibility of handling small samples. At the same time, a high level of performance is required, in particular in terms of (1) the detection limit, which must be as low as possible, (2) specificity, i.e., the ability to detect a particular molecule in a complex mixture, and (3) speed.
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References
Manz, A., Graber, N., Widmer, H.M.: Miniaturized total chemical analysis systems: A novel concept for chemical sensing, Sensors and Actuators B: Chemical 1 (1–6), 244–248 (1990)
Bange, A., Halsall, H.B., Heineman, W.R.: Microfluidic immunosensor systems, Biosensors and Bioelectronics 20, 2488–2503 (2005)
Vilkner, T., Janasek, D., Manz, A.: Micro total analysis systems. Recent developments, Analytical Chemistry 76 (12), 3373 (2004)
Reyes, D.R., et al.: Micro total analysis systems. 1. Introduction, Theory, and Technology, Analytical Chemistry 74 (12), 2623–2636 (2002)
Auroux, P.-A., et al.: Micro total analysis systems. 2. Analytical standard operations and applications, Analytical Chemistry 74 (12), 2637–2652 (2002)
Oosterbroek, R.E., van den Berg, A.: Lab-on-a-Chip, Miniaturized Systems for (Bio)Chemical Analysis and Synthesis, ed. by R.E. Oosterbroek and A. van den Berg, Elsevier (2003) p. 394
Tüdõs, A.J., Besselink, G.A.J., Schasfoort, R.B.M.: Trends in miniaturized total analysis systems for point-of-care testing in clinical chemistry, Lab on a Chip 1 (2), 83–95 (2001)
Hobson, N.S., Tothill, I., Turner, A.P.F.: Microbial detection, Biosensors and Bioelectronics 11 (5), 455–477 (1996)
Ahmed, F.E.: Detection of genetically modified organisms in foods, Trends in Biotechnology 20 (5), 215–223 (2002)
Koester, C.J., Simonich, S.L., Esser, B.K.: Environmental Analysis, Analytical Chemistry 75, 2813–2829 (2003)
Richardson, S.D.: Water analysis: Emerging contaminants and current issues, Analytical Chemistry 75 (12), 2831–2857 (2003)
Iqbal, S.S., et al.: A review of molecular recognition technologies for detection of biological threat agents, Biosensors and Bioelectronics 15 (11–12), 549–578 (2000)
Wang, J.: Microchip devices for detecting terrorist weapons, Analytica Chimica Acta 507 (1) 3–10 (2004)
Skelley, A.M., et al. Development and evaluation of a microdevice for amino acid biomarker detection and analysis on Mars, P.N.A.S. USA 102 (4), 1041–1046 (2005)
White, T.J.: The future of PRC technology: Diversification of technologies and applications, Trends in Biotechnology 14 (12), 478–483 (1996)
Le Pioufle, B., Frenea, M., Tixier, A.: Biopuces pour le traitement de cellules vivantes: Micromanipulation des cellules par voie électrique ou microfluidique, Comptes Rendus Physique 5 (5), 589–596 (2004)
El-Ali, J., Sorger, P.K., Jensen, K.F.: Cells on chips, Nature 442 (7101), 403 (2006)
Lichtenberg, J., de Rooij, N.F., Verpoorte, E.: Sample pretreatment on microfabricated devices, Talanta 56 (2), 233–266 (2002)
Pawliszyn, J.: Sample preparation: Quo Vadis?, Analytical Chemistry 75 (11), 2543–2558 (2003)
Andersson, H., et al.: Micromachined flow-through filter-chamber for chemical reactions on beads, Sensors and Actuators B: Chemical 67 (1–2), 203–208 (2000)
Xing, X., et al.: Micromachined membrane particle filters, Sensors and Actuators A: Physical 73 (1–2), 184–191 (1999)
Desai, T.A., et al.: Nanoporous anti-fouling silicon membranes for biosensor applications, Biosensors and Bioelectronics 15 (9–10), 453–462 (2000)
Brody, J.P., Yager, P.: Diffusion-based extraction in a microfabricated device, Sensors and Actuators A: Physical 58 (1), 13–18 (1997)
Hatch, A., et al.: A rapid diffusion immunoassay in a T-sensor, Nature Biotechnology 19 (5), 461–465 (2001)
Raymond, D.E., Manz, A., Widmer, H.M.: Continuous sample pretreatment using a free-flow electrophoresis device integrated onto a silicon chip, Anal. Chem. 66, 2858–2865 (1994)
Stachowiak, T.B., Svec, F., Frechet, J.M.J.: Chip electrochromatography, Journal of Chromatography A 1044 (1–2), 97–111 (2004)
Sarrut, N., et al.: Enzymatic digestion and liquid chromatography in micro-pillar reactors; hydrodynamic versus electroosmotic driven flow. In: Photonics West, Microfluidics, BioMEMS and Medical Microsystems III, San Jose, California: SPIE-Int. Soc. Opt. Eng. (2005)
Oleschuk, R.D., et al.: Trapping of bead-based reagents within microfluidic systems: On-chip solid-phase extraction and electrochromatography, Analytical Chemistry 72 (3), 585–590 (2000)
Yu, C., et al.: Monolithic porous polymer for on-chip solid-phase extraction and preconcentration prepared by photoinitiated in situ polymerization within a microfluidic device, Analytical Chemistry 73 (21), 5088–5096 (2001)
Stachowiak, T.B., et al.: Fabrication of porous polymer monoliths covalently attached to the walls of channels in plastic microdevices, Electrophoresis 24 (21), 3689–3693 (2003)
Lion, N., et al.: Microfluidic systems in proteomics, Electrophoresis 24 (21), 3533–3562 (2003)
Fan, Z.H., et al.: Dynamic DNA hybridization on a chip using paramagnetic beads, Analytical Chemistry 71 (21), 4851–4859 (1999)
Choi, J.-W., et al.: Development and characterization of microfluidic devices and systems for magnetic bead-based biochemical detection, Biomedical Microdevices 3 (3), 191–200 (2001)
Tokeshi, M., et al.: Continuous-flow chemical processing on a microchip by combining microunit operations and a multiphase flow network, Analytical Chemistry 74 (7), 1565–1571 (2002)
Hashimoto, M., et al.: Rapid PCR in a continuous flow device, Lab on a Chip 4 (6), 638–645 (2004)
Giordano, B.C., et al.: Polymerase chain reaction in polymeric microchips: DNA amplification in less than 240 seconds, Analytical Biochemistry 291 (1), 124–132 (2001)
Guijt, R.M., et al.: Chemical and physical processes for integrated temperature control in microfluidic devices, Lab on a Chip 3 (1), 1–4 (2003)
Roper, M.G., Easley, C.J., Landers, J.P.: Advances in polymerase chain reaction on microfluidic chips, Analytical Chemistry 77 (12), 3887–3893 (2005)
Schwarz, M.A., Hauser, P.C.: Recent developments in detection methods for microfabricated analytical devices, Lab on a Chip 1 (1), 1–6 (2001)
Fritz, J., et al.: Translating biomolecular recognition into nanomechanics, Science 288 (5464), 316–318 (2000)
Drummond, G., Hill, M.G., Barton, J.K.: Electrochemical DNA sensors, Nature Biotechnology 21 (10), 1192–1199 (2003)
Bakker, E., Qin, Y.: Electrochemical Sensors, Anal. Chem. 78 (12), 3965–3984 (2006)
Miller, M.M., et al.: Detection of a micron-sized magnetic sphere using a ring-shaped anisotropic magnetoresistance-based sensor: A model for a magnetoresistance-based biosensor, Applied Physics Letters 81 (12), 2211–2213 (2002)
Cui, Y., al.: Nanowire nanosensors for highly sensitive and selective detection of biological and chemical species, Science 293, 1289–1292 (2001)
Patolsky, F., Zheng, G., Lieber, C.M.: Nanowire-based biosensors, Analytical Chemistry 78 (13), 4261 (2006)
Gu, L.-Q., Cheley, S., Bayley, H.: Capture of a single molecule in a nanocavity, Science 291, 636–640 (2001)
Saleh, O.A., Sohn, L.L.: An artificial nanopore for molecular sensing, Nano Letters 3 (1), 37–38 (2003)
Gut, I.G.: Automation in genotyping of single nucleotide polymorphisms, Human Mutation 17, 475–492 (2001)
Aebersold, R., Mann M.: Mass spectrometry-based proteomics, Nature 422, 198–207 (2003)
Hierlemann, A., et al.: Microfabrication techniques for chemical/biosensors, Proceedings of the IEEE 91 (6), 839–863 (2003)
Verpoorte, E.M.J., de Rooij, N.F.: Microfluidics meets MEMS, Proceedings of the IEEE 91 (6), 930–953 (2003)
Becker, H., Locascio, L.E.: Polymer microfluidic devices, Talanta 56 (2), 221–378 (2002)
Spearing, S.M.: Materials issues in microelectromechanical systems (MEMS), Acta Materialia 48 (1), 179–196 (2000)
Kovacs, G.T.A., Maluf, N.I., Petersen, K.E.: Bulk micromachining of silicon, Proceedings of the IEEE 86 (8), 1536–1551 (1998)
Bustillo, J.M., Howe, R.T., Muller, R.S.: Surface micromachining for microelectromechanical systems, Proceedings of the IEEE 86 (8), 1552–1574 (1998)
Duffy, D.C., et al.: Rapid prototyping of microfluidic systems in poly (dimethylsiloxane), Analytical Chemistry 70 (23), 4974–4984 (1998)
Heckele, M., Schomburg, W.K.: Review on micro molding of thermoplastic polymers, Journal of Micromechanics and Microengineering 14 (3), R1–R14 (2004)
Noerholm, M., et al.: Polymer microfluidic chip for online monitoring of microarray hybridizations, Lab on a Chip 4 (1), 28–37 (2004)
Rossier, J.S., et al.: Plasma etched polymer microelectrochemical systems, Lab on a Chip 2 (3), 145–150 (2002)
Anderson, R.C., et al.: A miniature integrated device for automated multistep genetic assays, Nucleic Acids Research 28 (12), E60 (2000)
Liu, R.H., et al.: Self-contained, fully integrated biochip for sample preparation, polymerase chain reaction amplification, and DNA microarray detection, Analytical Chemistry 76 (7), 1824–1831 (2004)
Easley, C.J. et al.: A fully integrated microfluidic genetic analysis system with sample-in-answer-out capability, Proceedings of the National Academy of Sciences 103 (51), 19272–19277 (2006)
Hong, J.W., et al.: A nanoliter-scale nucleic acid processor with parallel architecture, Nature Biotechnology 22 (4), 435–439 (2004)
Hong, J.W., et al.: Molecular biology on a microfluidic chip, Journal of Physics: Condensed Matter 18 (18) (2006)
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Puget, P. (2009). Lab on a Chip. In: Boisseau, P., Houdy, P., Lahmani, M. (eds) Nanoscience. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-88633-4_20
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DOI: https://doi.org/10.1007/978-3-540-88633-4_20
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