Abstract
Multi-miRNA Hairpins technology refers to a single artificial construct that can produce multiple mature miRNAs, improving gene knockdown over single miRNAs and offering expression silence of multiple genes. This technology was concurrently developed in 2006 by Zhu's laboratory (Department of Developmental and Molecular Biology, Albert Einstein College of Medicine) [Sun D, Melegari M, Sridhar S, Rogler CE, Zhu L, Biotechniques 41:59–63, 2006] and by Xu's laboratory (Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School) [Xia XG, Zhou H, Xu Z, Biotechniques 41:64–68, 2006a]. Similar principle was later applied by my laboratory (Department of Medicine, Montreal Heart Institute, University of Montreal) in 2008 to miR-Mimics to establish the Multi-miRNA Mimics technology that is able to silence multiple genes [Chen G, Lin H, Xiao J, Luo X, Wang Z, Biotechniques 2009]. Both Multi-miRNA Hairpins and Multi-miRNA Mimics technologies belong to the “miRNA-targeting” and “miRNA-gain-of-function” strategy. These technologies were developed based on the concept ‘One-Drug, Multiple-Target’ described in Sect. 2. 3.
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Wang, Z. (2009). Multi-miRNA Hairpins and Multi-miRNA Mimics Technologies. In: MicroRNA Interference Technologies. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-00489-6_5
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DOI: https://doi.org/10.1007/978-3-642-00489-6_5
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