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Conformational Structure and Dynamics from Single-Molecule FRET

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Energy Transfer Dynamics in Biomaterial Systems

Part of the book series: Springer Series in Chemical Physics ((CHEMICAL,volume 93))

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Abstract

Various ways of extracting information on the conformational structure, dynamics and correlations between them from single-molecule measurements of orescence resonance energy transfer are surveyed. The information obtained via those various ways is then analyzed in detail in the case of an off-lattice model of a two-stranded coiled-coil polypeptide that follows Langevin dynamics. The analysis includes a consideration of the cases of a freely diffusing and surface-immobilized polypeptide as well as the effect of different types of surface and denaturation conditions.

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Author information

Authors and Affiliations

  1. Chemistry Department, University of Michigan, 930 N. University Ave, Ann Arbor, MI, 48109-1055, USA

    Eitan Geva & Jianyuan Shang

Authors
  1. Eitan Geva
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  2. Jianyuan Shang
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Correspondence to Eitan Geva .

Editor information

Editors and Affiliations

  1. Department de chimie, UMR 8642 due CNRS, Ecole Normale Superieure, rue Lhomond 24, Paris CX 05, 75231, France

    Irene Burghardt

  2. Institut für Physik, AG Halbleitertheorie, Humboldt-Univ. Berlin, Newtonstr. 15, Berlin, 12489, Germany

    V. May

  3. Physical Chemistry Division, University Florida, New Physics Building 2318, Gainesville, 32611-8435, USA

    David A. Micha

  4. Dept. Chemistry, University of Houston, Houston, 77204-5003, USA

    E. R. Bittner

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Geva, E., Shang, J. (2009). Conformational Structure and Dynamics from Single-Molecule FRET. In: Burghardt, I., May, V., Micha, D., Bittner, E. (eds) Energy Transfer Dynamics in Biomaterial Systems. Springer Series in Chemical Physics, vol 93. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-02306-4_3

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