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Image Formation and Analysis of Coherent Microscopy and Beyond – Toward Better Imaging and Phase Recovery

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Coherent Light Microscopy

Part of the book series: Springer Series in Surface Sciences ((SSSUR,volume 46))

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Abstract

Applications of phase microscopy based on either coherent or partially coherent sources are widely distributed in today's biological and biomedical research laboratories. But the quantitative phase information derivable from these techniques is often not fully understood, because in general, no universal theoretical model can be set up, and each of the techniques has to be treated specifically. This chapter is dedicated to the fundamental understanding of the methodologies that derive optical phase information using imaging techniques and microscopic instrumentation. Several of the latest and most significant techniques are thoroughly studied through the theoretical formalism of the optical transfer function. In particular, we classify these systems into two main categories: those based on coherent illumination, such as digital holographic microscopy (DHM) and its extension into tomography, and those based on partially coherent illumination, such as differential interference contrast (DIC) and differential phase contrast (DPC). Our intention is that the models described in this chapter give an insight into the behaviour of these phase imaging techniques, so that better instrumentation can be designed and improved phase retrieval algorithms can be devised.

The authors Shan Shan Kou and Shalin B. Mehta have contributed equally to the work.

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Authors and Affiliations

  1. Division of Bioengineering, Optical Bioimaging Laboratory, National University of Singapore, Singapore, 117576, Singapore

    Shan Shan Kou, Shalin B. Mehta & Shakil Rehman

  2. Graduate School for Integrative Sciences and Engineering (NGS), National University of Singapore, Singapore, 117597, Singapore

    Shan Shan Kou & Shalin B. Mehta

  3. Singapore Eye Research Institute, Singapore, 168751, Singapore

    Shakil Rehman

  4. Division of Bioengineering, Optical Bioimaging Laboratory, National University of Singapore, 7 Engineering Drive 1, Singapore, 117576, Singapore

    Colin J.R. Sheppard

  5. Graduate School for Integrative Sciences and Engineering (NGS), National University of Singapore, 10 Medical Drive, Singapore, 117597, Singapore

    Colin J.R. Sheppard

  6. Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore, 117543, Singapore

    Colin J.R. Sheppard

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  1. Shan Shan Kou
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  2. Shalin B. Mehta
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Correspondence to Colin J.R. Sheppard .

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  1. Ist. Nazionale di Ottica Applicata, CNR, Via Campi Flegrei 34, Pozzuoli, 80078, Napoli, Italy

    Pietro Ferraro

  2. Dept. Biomedical Engineering, Duke University, Durham, 27708-0292, North Carolina, USA

    Adam Wax

  3. Dept. Engineering, Bar-Ilan University, Ramat Gan, 52900, Israel

    Zeev Zalevsky

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Kou, S.S., Mehta, S.B., Rehman, S., Sheppard, C.J. (2011). Image Formation and Analysis of Coherent Microscopy and Beyond – Toward Better Imaging and Phase Recovery. In: Ferraro, P., Wax, A., Zalevsky, Z. (eds) Coherent Light Microscopy. Springer Series in Surface Sciences, vol 46. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-15813-1_11

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