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The Integration of Recognition and Cleavage: X-Ray Structures of Pre-Transition State Complex, Post-Reactive Complex, and the DNA-Free Endonuclease

  • Chapter
Restriction Endonucleases

Part of the book series: Nucleic Acids and Molecular Biology ((NUCLEIC,volume 14))

Abstract

DNA has been selected as the biological information storage molecule for many reasons; one of which is its stability. The rate of spontaneous hydrolysis of DNA(at 24 ° C and pH 7.4) was estimated at 5.7×10−14 S−1 (Bunton et al.1960; Kumamoto et al. 1956; Serpersu et al. 1987); more recently, Radzicka and Wolfenden have estimated this value as 1.7xl0−13 S−1. (Radzicka and Wolfenden 1995).This corresponds to an estimated half-life of 130,000 years for a DNA phosphodiester bond in solution, placing DNAhydrolysis among the slowest of biochemical reactions in the absence of enzymes.

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Authors and Affiliations

  1. Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, 15260, USA

    A. Grigorescu, M. Horvath, P. A. Wilkosz, K. Chandrasekhar & J. M. Rosenberg

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  1. A. Grigorescu
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  2. M. Horvath
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  3. P. A. Wilkosz
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  4. K. Chandrasekhar
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  1. Institute for Biochemistry, Justus-Liebig-University, Heinrich-Buff-Ring 58, 35392, Giessen, Germany

    Alfred M. Pingoud

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Grigorescu, A., Horvath, M., Wilkosz, P.A., Chandrasekhar, K., Rosenberg, J.M. (2004). The Integration of Recognition and Cleavage: X-Ray Structures of Pre-Transition State Complex, Post-Reactive Complex, and the DNA-Free Endonuclease. In: Pingoud, A.M. (eds) Restriction Endonucleases. Nucleic Acids and Molecular Biology, vol 14. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-18851-0_6

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