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Magneto-Nanosensor Diagnostic Chips

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Point-of-Care Diagnostics on a Chip

Part of the book series: Biological and Medical Physics, Biomedical Engineering ((BIOMEDICAL))

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Abstract

We have developed an automated assay for disease biomarker detection that can be run on a handheld sensing platform. By coupling magnetic nanotechnology with an array of magnetically responsive nanosensors, we demonstrate a rapid, multiplex immunoassay that eliminates the need for trained technicians to run molecular diagnostic tests. A major limitation for other detection modalities is signal distortion that occurs due to background heterogeneity in ionic strength, pH, temperature, and autofluorescence. Here, we present a magnetic nanosensor technology that is insensitive to background yet still capable of rapid, multiplex protein detection with resolution down to attomolar concentrations and extensive linear dynamic range. The insensitivity of our detector to various media enables our technology to be directly applied to a variety of settings such as molecular biology and clinical diagnostics.

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Acknowledgements

This work was supported, in part, by the United States National Cancer Institute (grants 1U54CA119367, 1U54CA143907, 1U54CA151459, 1U01CA152737, 5R33CA138330, and N44CM-2009–00011), the United States National Science Foundation (grant ECCS-0801385–000), the United States Defense Advanced Research Projects Agency/Navy (grant N00014–02–1–0807), a Gates Foundation Grand Challenge Exploration Award, and the National Semiconductor Corporation. RSG acknowledges financial support from the Stanford Medical School Medical Scientist Training Program and the National Science Foundation Graduate Research Fellowship Program.

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Authors and Affiliations

  1. Department of Bioengineering, Medical Scientist Training Program, School of Medicine, Stanford, CA, 94305, USA

    Richard S. Gaster

  2. Department of Electrical Engineering, Stanford University, Stanford, CA, 94305, USA

    Drew A. Hall & Shan X. Wang

  3. Department of Materials Science and Engineering, Stanford University, Stanford, CA, 94305, USA

    Shan X. Wang

Authors
  1. Richard S. Gaster
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  2. Drew A. Hall
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  3. Shan X. Wang
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Correspondence to Shan X. Wang .

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Editors and Affiliations

  1. , Department of Bioengineering, University of Pennsylvania, 210 South 33rd Street, Philadelphia, 19104-6321, Pennsylvania, USA

    David Issadore

  2. , School of Engineering, Applied Sciences, Harvard University, Oxford Street 29, Cambridge, 02138, Massachusetts, USA

    Robert M. Westervelt

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Gaster, R.S., Hall, D.A., Wang, S.X. (2013). Magneto-Nanosensor Diagnostic Chips. In: Issadore, D., Westervelt, R. (eds) Point-of-Care Diagnostics on a Chip. Biological and Medical Physics, Biomedical Engineering. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-29268-2_7

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  • DOI: https://doi.org/10.1007/978-3-642-29268-2_7

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-29267-5

  • Online ISBN: 978-3-642-29268-2

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