Skip to main content
SpringerLink
Log in

Enzymes of the Pentose Phosphate Cycle

  • Chapter
Modern Methods of Plant Analysis / Moderne Methoden der Pflanzenanalyse

Part of the book series: Modern Methods of Plant Analysis / Moderne Methoden der Pflanzenanalyse ((PFLANZENANAL.,volume 7))

  • 229 Accesses

Abstract

The authors have reviewed the literature covering the methods used in the isolation, purification, and assay of the ten enzymes of the pentose phosphate cycle of metabolism in higher plants, as well as the preparation of substrates. Where detailed investigations of higher plants are lacking, information from studies on microorganisms and animal tissues has been included since the procedures are generally applicable.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Abbreviations

G-6-P:

dehydrogenase, glucose-6-phosphate dehydrogenase

6-PGA:

dehydrogenase, 6-phosphogluconate dehydrogenase

DAP:

dihydroxyacetone phosphate

F-6-P:

fructose-6-phosphate

F-1,6-P:

fructose-1,6-diphosphate

GSH:

reduced glutathione

GSSG:

oxidized glutathione

G-6-P:

glucose-6-phosphate

G-3-P:

glyceraldehyde-3-phosphate

PEP:

phosphoenolpyruvate

6-PGA:

6-phosphogluconate

R-5-P:

ribose-5-phosphate

Ru-5-P:

ribulose-5-phosphate

Ru-1,5-P:

ribulose-1,5-diphosphate

S-7-P:

sedoheptulose-7-phophate

Xu-5-P:

xylulose-5-phophate

ADP:

adenosine diphosphate

ATP:

adenosine triphosphate

DPN:

disphosphopyridine nucleaotide

DPNH:

reduced disphosphopyridine nucleotide

TPN:

triphosphopyridine nucleaotide

TPNH:

reduced diphosphopyridine nucleaotide

TPP:

thiaminepyrophosphate

EDTA:

ethylenediaminotetra acetic acid

DEAE:

cellulose, diethylaminoethycellulose

S.A. (s.a.):

Specific activity

TCA:

trichloracetic acid

Tris, Tris:

(hydroxy)-methylaminomethane

TTC:

triphenyltetrazolium chloride

References

  1. Anderson, D. G., H. A. Stafford, E. E. Conn and B. VeNnesland: Plant Physiol. 27, 675 (1952).

    PubMed  CAS  Google Scholar 

  2. Ashwell, G., and J. J. Hickman: J. Amer. chem. Soc. 76, 5889 (1954).

    CAS  Google Scholar 

  3. Axelrod, B., R. S. Bandurski, C. M. Greiner and R. Jang: J. biol. Chem. 202, 619 (1953).

    PubMed  CAS  Google Scholar 

  4. Axelrod, B., and H. Beevers: Ann. Rev. PL Physiol. 7, 267 (1956).

    CAS  Google Scholar 

  5. Axelrod, B., and R. Jang: J. biol. Chem. 209, 847 (1954).

    PubMed  CAS  Google Scholar 

  6. Bailey, R. C, and E. C. Webb: Biochem. J. 42, 60 (1948).

    CAS  Google Scholar 

  7. Barnett, R. C., H. A. Stafford, E. E. Conn and B. VeNnesland: Plant Physiol. 28, 115 (1953).

    PubMed  CAS  Google Scholar 

  8. Berger, L., M. W. Slein, S. P. Colowick and C. F. Cori: J. gen. Physiol. 29, 379 (1946).

    CAS  Google Scholar 

  9. Boser, H.: Phytopath. Z. 33, 197 (1958).

    Google Scholar 

  10. Brodie, A. P., and F. Lipmann: J. biol. Chem. 212, 677 (1955).

    PubMed  CAS  Google Scholar 

  11. Cardini, C. E.: Enzymologia 14, 362 (1950).

    Google Scholar 

  12. Clayton, R. A.: Arch. Biochem. Biophys. 79, 111 (1959).

    CAS  Google Scholar 

  13. Colowick, S. P., and H. M. Kalckar: J. biol. Chem. 148, 117 (1943).

    CAS  Google Scholar 

  14. Conn, E. E., and B. VeNnesland: J. biol. Chem. 192, 17 (1951).

    PubMed  CAS  Google Scholar 

  15. Cori, O., and F. Lipmann: J. biol. Chem. 194, 417 (1952).

    PubMed  CAS  Google Scholar 

  16. Cori, G. T., M. W. Slein and C. F. Cori: J. biol. Chem. 173, 605 (1948).

    PubMed  CAS  Google Scholar 

  17. Couri, D., and E. Racker: Arch. Biochem. Biophys. 83, 195 (1959).

    PubMed  CAS  Google Scholar 

  18. DE LA Haba, G., I. G. Leder and E. Racker: J. biol. Chem. 214, 409 (1955).

    Google Scholar 

  19. DE LA Haba, G., and E. Racker: Methods in Enzymology 1, 375 (1955).

    Google Scholar 

  20. Deley, J.: Enzymologia 18, 33 (1957).

    CAS  Google Scholar 

  21. DE Moss, R. D.: Methods in Enzymology 1, 328 (1955).

    Google Scholar 

  22. Dickens, F.: Ann. N.Y. Acad. Sci. 75, 71 (1958).

    PubMed  CAS  Google Scholar 

  23. Dickens, F., and D. H. Williamson: Nature (Lond.) 176, 400 (1955).

    CAS  Google Scholar 

  24. Dische, Z.: J. biol. Chem. 204, 983 (1953).

    PubMed  CAS  Google Scholar 

  25. Dische, Z., and E. Borenfrettnd: J. biol. Chem. 192, 583 (1951).

    PubMed  CAS  Google Scholar 

  26. Forti, G., C. Tua, and L. Tognoli: Biochim. biophys. Acta 36, 19 (1959).

    PubMed  CAS  Google Scholar 

  27. Fujita, A., and I. Numata: Biochem. Z. 299, 249 (1938).

    Google Scholar 

  28. Gibbs, M.: Nature (Lond.) 170, 164 (1952).

    CAS  Google Scholar 

  29. Gibbs, M.: Plant Physiol. 29, 34 (1954).

    PubMed  CAS  Google Scholar 

  30. Gibbs, M.: Ann. Rev. PL Physiol. 10, 329 (1959).

    CAS  Google Scholar 

  31. Gibbs, M., and B. L. Horecker: J. biol. Chem. 208, 813 (1954).

    PubMed  CAS  Google Scholar 

  32. Gottschalk, A.: Biochem. J. 41, 478 (1947).

    CAS  Google Scholar 

  33. Hageman, R. H., and E. R. Waygood: Plant Physiol. 34, 306 (1959).

    Google Scholar 

  34. Hann, R. M., E. B. Tilden and C. S. Hudson: J. Amer. chem. Soc. 60, 1201 (1938).

    CAS  Google Scholar 

  35. Hers, H. G.: Biochim. biophys. Acta 8, 416 (1952).

    PubMed  CAS  Google Scholar 

  36. Hers, H. G.: Biochim. biophys. Acta 8, 424 (1952).

    PubMed  CAS  Google Scholar 

  37. Hers, H. G.: Methods in Enzymology 1, 289 (1955).

    Google Scholar 

  38. Hochster, R. M.: Canad. J. Microbiol. 1, 346 (1954-1955).

    Google Scholar 

  39. Horecker, B. L.: Methods in Enzymology 11, 195 (1957).

    Google Scholar 

  40. Horecker, B. L., M. Gibbs, H. Klenow and P. Z. Smyrniotis: J. biol. Chem. 206, 393 (1954).

    Google Scholar 

  41. Horecker, B. L., and P. Z. Smyrniotis: J. biol. Chem. 193, 371 (1951).

    PubMed  CAS  Google Scholar 

  42. Horecker, B. L., and P. Z. Smyrniotis: J. Amer. chem. Soc. 75, 2021 (1953).

    CAS  Google Scholar 

  43. Horecker, B. L., and P. Z. Smyrniotis: J. biol. Chem. 212, 811 (1955).

    PubMed  CAS  Google Scholar 

  44. Horecker, B. L., and P. Z. Smyrniotis: Methods in Enzymology 1, 323 (1955a).

    Google Scholar 

  45. Horecker, B. L., and P. Z. Smyrniotis: Methods in Enzymology 1, 371 (1955b).

    CAS  Google Scholar 

  46. Horecker, B. L., and P. Z. Smyrniotis: Methods in Enzymology 1, 381 (1955c).

    CAS  Google Scholar 

  47. Horecker, B. L., P. Z. Smyrniotis, H. H. Hiatt and P. A. Marks: J. biol. Chem. 212, 827 (1955).

    PubMed  CAS  Google Scholar 

  48. Horecker, B. L., P. Z. Smyrniotis and J. Httrwitz: J. biol. Chem. 223, 1009 (1956).

    PubMed  CAS  Google Scholar 

  49. Horecker, B. L., P. Z. Smyrniotis and H. Klenow: J. biol. Chem. 205, 661 (1953).

    PubMed  CAS  Google Scholar 

  50. Horecker, B. L., P. Z. Smyrniotis and G. S. Seegmiller: J. biol. Chem. 193, 383 (1951).

    PubMed  CAS  Google Scholar 

  51. Htjrwitz, J., and B. L. Horecker: J. biol. Chem. 223, 993 (1956).

    Google Scholar 

  52. Htjrwitz, J., A. Weissbach, B. L. Horecker and P. Z. Smyrniotis: J. biol. Chem. 218, 769 (1956).

    Google Scholar 

  53. James, W. O.: Adv. Enzymol. 18, 281 (1957).

    CAS  Google Scholar 

  54. Kehjn, D., and E. F. Habtbee: Proc. Roy. Soc. (Lond.) Ser. B124,397 (1937-1938).

    Google Scholar 

  55. Klevestband, R., and A. Nobdal: Acta chem. scand. 4, 1320 (1950).

    Google Scholar 

  56. KoBn-bebg, A.: J. biol. Chem. 182, 805 (1950).

    Google Scholar 

  57. KoBnbebg, A., and B. L. Hobeckeb: Methods in Enzymology 1, 323 (1955).

    Google Scholar 

  58. KoBnbebg, A., and W. E. Pbiceb: J. biol. Chem. 193, 481 (1951).

    Google Scholar 

  59. Kbebs, E. G.: Methods in Enzymology 1, 407 (1955).

    Google Scholar 

  60. Kbebs, H. A., and H. L. KOBNBEBG: Ergebn. Physiol. 29, 212 (1957).

    Google Scholar 

  61. Ktjnitz, M., and M. R. Mcdonald: J. gen. Physiol. 29, 393 (1946).

    Google Scholar 

  62. Ktjnitz, M., and M. R. Mcdonald: in “Crystalline Enzymes”, ed. J. H. Nobtheop, p. 181 2nd. ed. New York: Columbia University Press 1948.

    Google Scholar 

  63. Kubsanov, A. L., O. A. Pavlinova and T. P. AfAnasieva: Arch. Bioehem. Biophys. 83, 239 (1959).

    Google Scholar 

  64. Labdy, H. A.: “Respiratory enzymes,” rev. ed. p. 184. Minneapolis: Burgess 1949.

    Google Scholar 

  65. LeTTthabt, F., and E. Testa: Helv. physiol. Acta 8, C67 (1950).

    Google Scholar 

  66. Mcdonald, M. R.: Methods in Enzymology 1, 269 (1955).

    CAS  Google Scholar 

  67. Medina, A., and D. J. D. Nicholas: Bioehem. J. 66, 573 (1957).

    CAS  Google Scholar 

  68. Medina, A., and A. Sols: Biochim. biophys. Acta 19, 378 (1956).

    PubMed  CAS  Google Scholar 

  69. Mejbaum, W.: Hoppe-Seylers Z. physiol. Chem. 258, 117 (1939).

    CAS  Google Scholar 

  70. Meyebhof, O.: Bioehem. Z. 183, 176 (1927).

    Google Scholar 

  71. Negelein, E., and E. Haas: Bioehem. Z. 282, 206 (1935).

    CAS  Google Scholar 

  72. Neilands, J. B., and P. K. Stumpf: “Enzyme chemistry,” 2nd ed. New York: John Wiley and Sons 1958.

    Google Scholar 

  73. Nelson, N.: J. biol. Chem. 153, 375 (1944).

    CAS  Google Scholar 

  74. NETTFELD, E. F., N. O. KAPLAN and S. P. COLOWICK: Biochim. biophys. Acta 17, 525 (1955).

    Google Scholar 

  75. Pabks, R. E., E. Ben-gebshom and H. A. Labdy: J. biol. Chem. 227, 231 (1957).

    Google Scholar 

  76. Rackeb, E.: J. biol. Chem. 167, 843 (1947).

    Google Scholar 

  77. Rackeb, E., G. DE LA Haba and I. G. Ledeb: J. Amer. chem. Soc. 75, 1010 (1953).

    Google Scholar 

  78. Rackeb, E., and E. A. R. Schboe-deb: Arch. Bioehem. Biophys. 66, 241 (1957).

    Google Scholar 

  79. Radhakbishnan, A. N.: Biochim. biophys. Acta 40, 546 (1960).

    Google Scholar 

  80. RamAsabma, T., and K. V. Gnti: Arch. Bioehem. Biophys. 62, 91 (1956).

    Google Scholar 

  81. Richteb, G.: Z. Naturforsch. 12b, 662 (1957).

    Google Scholar 

  82. Saltman, P.: J. biol. Chem. 200, 145 (1953).

    PubMed  CAS  Google Scholar 

  83. Sebvettaz, O.: R. C. Accad. Lincei 20, 255 (1956).

    Google Scholar 

  84. Simpson, P. J.: Canad. J. Bioehem. Physiol. 38, 115 (1960).

    CAS  Google Scholar 

  85. Slein, M. W.: Methods in Enzymology 1, 299 (1955).

    CAS  Google Scholar 

  86. Slein, M. W., G. T. Cobiand C. F. Cobi: J. biol. Chem. 186, 763 (1950).

    PubMed  CAS  Google Scholar 

  87. Sols, A.: Biochim. biophys. Acta 19, 144 (1956).

    PubMed  CAS  Google Scholar 

  88. Somebs, G. F., and E. Cosby: Arch. Bioehem. 6, 295 (1945).

    Google Scholar 

  89. Somogyi, M.: J. biol. Chem. 160, 69 (1945).

    CAS  Google Scholar 

  90. Spybidon, G. A. A., and O. F. Denstedt: J. biol. Chem. 199, 493 (1952).

    Google Scholar 

  91. Sbebe, P. A., J. R. Coopeb, V. Klybas and E. Rackeb: Arch. Bioehem. Biophys. 59, 535 (1955).

    Google Scholar 

  92. Sbebe, P. A., J. R. Cooper, M. Tabachnick and E. Racker: Arch. Bioehem. Biophys. 74, 295 (1958).

    Google Scholar 

  93. Sbebe, P. A., H. L. KoBnbebg and E. Rackeb: Fed. Proc. 14, 285 (1955).

    Google Scholar 

  94. Stumpf, P. K., and B. L. Hobeckeb: J. biol. Chem. 218, 753 (1956).

    PubMed  CAS  Google Scholar 

  95. Tabachnick, M., P. A. Sbebe, J. R. Coopeb and E. Rackeb: Arch. Bioehem. Biophys. 74, 315 (1958).

    CAS  Google Scholar 

  96. Tanko, B.: Bioehem. J. 30, 692 (1936).

    CAS  Google Scholar 

  97. Vallee, B. L., F. L. Hoch, S. J. Adelstein and W. E. C. Wackeb: J. Amer. chem. Soc. 78, 5879 (1956).

    CAS  Google Scholar 

  98. Vishniac, W.: Arch. Bioehem. 26, 167 (1950).

    CAS  Google Scholar 

  99. WaGneb-jatibegg, T., and H. Ratten: Hoppe-Seylers Z. physiol. Chem. 233, 215 (1935).

    Google Scholar 

  100. Wajzeb, J.: C. R. Acad. Sci. (Paris) 229, 1270 (1949).

    Google Scholar 

  101. WaBbttbg, O., and W. Christian: Bioehem. Z. 287, 291 (1936).

    Google Scholar 

  102. WaBbttbg, O., W. Christian and A. Gbiese: Bioehem. Z. 282, 157 (1935).

    Google Scholar 

  103. Wills, E. D., and A. Wobmall: Bioehem. J. 47, 158 (1950).

    CAS  Google Scholar 

  104. Wolin, M. J., F. J. Simpson and W. A. Wood: J. biol. Chem. 232, 559 (1958).

    PubMed  CAS  Google Scholar 

Download references

Authors
  1. E. R. Waygood
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Rohringer
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Microbiology, University of Manitoba, Winnipeg, Canada

    B. D. Sanwal Dr. (Associate Professor) (Associate Professor)

Rights and permissions

Reprints and permissions

Copyright information

© 1964 Springer-Verlag OHG. Berlin · Göttingen · Heidelberg

About this chapter

Cite this chapter

Waygood, E.R., Rohringer, R. (1964). Enzymes of the Pentose Phosphate Cycle. In: Linskens, H.F., Sanwal, B.D., Tracey, M.V. (eds) Modern Methods of Plant Analysis / Moderne Methoden der Pflanzenanalyse. Modern Methods of Plant Analysis / Moderne Methoden der Pflanzenanalyse, vol 7. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-48141-3_20

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-48141-3_20

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-48143-7

  • Online ISBN: 978-3-642-48141-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation