Abstract
Group B streptococci (GBS), or Streptococcus agalactiae, has remained the leading cause of bacterial sepsis and meningitis in neonates for the last two decades [1]. The incidence of perinatal group B streptococcal disease in the United States has been decreasing because intrapartum antibiotic prophylaxis has been widely used for prevention of GBS disease [2]. Identification of GBS-colonized women is critical for prevention of neonatal GBS infections. Currently, prenatal screening culture, including broth culture in selective medium, is the gold standard method for detection of anogenital GBS colonization [1]. However, culture methods require up to 48 h to yield results and only predict 87% of women likely to be colonized by GBS at delivery [3]. A rapid, sensitive and specific test for detection of GBS directly from clinical specimens would allow for a simpler and more efficient prevention program.
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References
Centers for Disease Control and Prevention (1996) Prevention of perinatal group B streptococcal disease: a public health perspective (RR-27). MMWR Morb Mortal Wkly Rep 45: 1–24
Schrag SJ, Zywicki S, Farley MM, Reingold AL, Harrison LH, Lefkowitz LB, Hadler JL, Danila R, Cieslak PR, Schuchat A (2000) Group B streptococcal disease in the era of intrapartum antibiotic prophylaxis. N Engl J Med 342: 15–20
Yancey MK, Schuchat A, Brown LK, Lee Venture V, Markenson GR (1996) The accuracy of late antenatal screening cultures in predicting genital group B streptococcal colonization at delivery. Obstet Gynecol 88: 811–815
Baker CJ (1996) Inadequacy of rapid immunoassays for intrapartum detection of group B streptococcal carriers. Obstet Gynecol 88: 51–55
Bourbeau PP, Heiter BJ, Figdore M (1997) Use of Gen-Probe AccuProbe Group B streptococcus test to detect group B streptococci in broth cultures of vaginal-anorectal specimens from pregnant women: comparison with traditional culture method. J Clin Microbiol 35: 144–147
Backman A, Lantz P-G, Râdstrom P, Olcén P (1999) Evaluation of an extended diagnostic PCR assay for detection and verification of the common causes of bacterial meningitis in CSF and other biological samples. Mol Cell Probes 13: 49–60
Mawn JA, Simpson AJ, Heard AJ (1993) Detection of the C protein gene among group B streptococci using PCR. J Clin Pathol 46: 633–636
Podbielski A, Blankenstein O, Lutticken R (1994) Molecular characterization of the cf b gene encoding group B streptococcal CAMP-factor. Med Microbiol Immunol 183: 239–256
Rosenstraus M, Wang Z, Chang SY, DeBonville D, Spadoro JP (1998) An internal control for routine diagnostic PCR: design, properties, and effect on clinical performance. J Clin Microbiol 36: 191–197
Neumaier M, Braun A, Wagener C (1998) Fundamentals of quality assessment of molecular amplification methods in clinical diagnostics. International Federation of Clinical Chemistry Scientific Division Committee on Molecular Biology Techniques. Clin Chem 44: 12–26
Ke D, Ménard C, Picard FJ, Boissinot M, Ouellette M, Roy PH, Bergeron MG (2000) Development of conventional and real-time PCR assays for the rapid detection of group B streptococci. Clin Chem 46: 324–331
Bergeron MG, Ke D, Ménard C, Picard FJ, Gagnon M, Bernier M, Ouellette M, Roy PH, Marcoux S, Fraser WD (2000) Rapid detection of group B streptococci in pregnant women at delivery (see comments). N Engl J Med 343: 175–179
Rosa-Fraile M, Rodriguez-Granger J, Cueto-Lopez M, Sampedro A, Gaye EB, Haro JM, Andreu A (1999) Use of Granada medium to detect group B streptococcal colonization in pregnant women. J Clin Microbiol 37: 2674–2677
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© 2002 Springer-Verlag Berlin Heidelberg
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Ke, D., Ménard, C., Picard, F.J., Bergeron, M.G. (2002). Rapid Detection of Group B Streptococci Using the LightCycler Instrument. In: Reischl, U., Wittwer, C., Cockerill, F. (eds) Rapid Cycle Real-Time PCR — Methods and Applications. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-48351-6_9
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DOI: https://doi.org/10.1007/978-3-642-48351-6_9
Publisher Name: Springer, Berlin, Heidelberg
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