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Genetic Transformation of Pea (Pisum sativum)

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Transgenic Crops II

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 47))

Abstract

Several approaches have been used to study plant tissue differentiation processes. Wounding, such as organ explantation in vitro (e.g., Lutova and Zabelina 1988) and exogenous phytohormone application (Gamborg et al. 1974; Kartha et al. 1974; Malmberg 1979; Hussey and Gunn 1984; Rubluo et al. 1984; Ezhova et al. 1985) showed that the main morphogenetic processes were directed by auxins and cytokinins (auxin/cytokinin ratio) and depended on internal factors (plant genotype, age and tissue), which can determine the endogenous phytohormonal balance of plant tissue and its susceptibility to phytohormones. Transformation with Agrobacterium tumefaciens and A. rhizogenes strains, which introduce bacterial phytohormonal genes into the plant genome, allows the study of plant morphogenetic responses to directed changing of the endogenous hormonal balance (Medford et al. 1989; Sitbon et al.1991). Transformation can also be used as an inducing condition to reveal differences between genetic forms in traits which characterize plant hormonal status. This approach reveals forms with atypical responses to transformation, i.e., potential hormonal mutants.

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Authors and Affiliations

  1. Dept. of Genetics, St. Petersburg State University, Universitetskaya emb., 7/9, 199034, St. Petersburg, Russia

    N. V. Malysheva, Z. B. Pavlova, N. S. Chernysh & L. A. Lutova

  2. Lab. of Biotechnology, All-Russian Institute of Agricultural Microbiology, sh. Podbelskiy 3, 189620 Pushkin 8, St. Petersburg, Russia

    L. V. Kravchenko

  3. Lab. of Plant Growth Regulators, All-Russian Institute of Plant Protection, sh. Podbelskiy 3, 189620 Pushkin 8, St. Petersburg, Russia

    Y. N. Kislin

  4. Lab. of Biochemistry, All-Russian Plant Research Institute, Isaakievskaya sq. 2, 199000, St. Petersburg, Russia

    V. Chmelev

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  1. N. V. Malysheva
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  2. Z. B. Pavlova
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  3. N. S. Chernysh
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  4. L. V. Kravchenko
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  5. Y. N. Kislin
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  6. V. Chmelev
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  7. L. A. Lutova
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  1. New Delhi, India

    Y. P. S. Bajaj

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Malysheva, N.V. et al. (2001). Genetic Transformation of Pea (Pisum sativum). In: Bajaj, Y.P.S. (eds) Transgenic Crops II. Biotechnology in Agriculture and Forestry, vol 47. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56901-2_19

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  • DOI: https://doi.org/10.1007/978-3-642-56901-2_19

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  • Print ISBN: 978-3-642-63130-6

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