Abstract
The correlated data obtained from multicolour, multiparameter flow cytometric analysis provides an accurate method to characterize sub populations of cells. Multicolour immuno fluorescence is also applicable where cell numbers are very low but there is a requirement for the determination of many specific cell surface markers, such as fine needle aspirates or bone marrow specimens. Commercial flow cytometers (both the sorters and the benchtop analysers) are available with two or more lasers which permits the use of multiple excitation wavelengths and a wider range of fluorochromes.
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References
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Beavis AJ and Pennline KJ. Detection of cell surface antigens using antibody-conjugated fluorospheres (ACF): Application for Six-Colour immuno fluorescence. BioTechniques 21: 498–503; 1996.
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© 2000 Springer-Verlag Berlin Heidelberg
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Beavis, A. (2000). Phenotypic Analysis Using Five-Colour Immunofluorescence and Flow Cytometry. In: Diamond, R.A., Demaggio, S. (eds) In Living Color. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57049-0_15
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DOI: https://doi.org/10.1007/978-3-642-57049-0_15
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-62978-5
Online ISBN: 978-3-642-57049-0
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