Abstract
The polymerase chain reaction (PCR) is a powerfulin vitromethod in molecular biology for selective, highly specific and exceptionally efficient amplification of nucleic acid sequences. In the 10 years since the first publication on PCR (Saiki et al., 1985) this method has grown to rival in popularity traditional microbiological, genetic and technical procedures for cloning, sequencing, gene detection and related procedures. Furthermore, in the meantime PCR and all of its different applications are rapid and convenient alternatives to traditional procedures such as blotting technologies, conventional hybridization and molecular cloning. Initially, PCR was a rather complex and tricky generic procedure applied to basic research problems in molecular biology. It has developed into a simple, multipurpose procedure more or less optimized for diverse applications in nearly every biological discipline and commercial area. There are frequent instances of PCR techniques having passed into the service laboratory environment. These service laboratories are providing a broad range of diagnostic tests mainly covering medical and forensic applications, but also environmental, agricultural and veterinary topics.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Akane A, Shiono H, Matsubara K, Nakamura H, Hasegawa M, Kagawa M. Purificaton of forensic specimens for the polymerase chain reaction (PCR) analysis. J Forensic Sci 1993, 38: 691–701
Barnes WM. PCR amplification of up to 35-kb DNA with high fidelity and high yield from kbacteriophage templates. Proc Natl Acad Sci USA 1994, 91: 2216–2220
Bauer P, Rolfs A, Regitz-Zagrosek V, Hildebrand A, Fleck E. MMLV reverse transcriptase creates PCR artifacts after effective DNase I digestion of purified RNA. BioTechniques1997, 22: 1128–1132
Becker Y, Darai G (eds.). PCR: protocols for diagnosis of human and animal virus dis-ease. Springer Verlag, Berlin, Heidelberg, New York, 1995 (ISBN 3–540–58899-X)
Cheng S, Fockler C, Barnes WM, Higuchi R. Effective amplification of long targets from cloned inserts and human genomic DNA. Proc Natl Acad Sci USA 1994, 91: 5695–5699
Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidium thio-cyanate-phenol-chloroform extration. Anal Biochem 1987, 162: 156–169
Clewley JP (ed.). The polymerase chain reaction (PCR) for human viral diagnosis. CRCPress, Boca Raton, Ann Arbor, 1995 (ISBN 0–8493–0–8493)
Dieffenbach CW, Dveksler GS (eds.). PCR primer: a laboratory manual. CSHL Press, New York, 1995 (ISBN 0–87969–0–87969)
Dutton CM, Paynton C, Sommer SS. General method for amplifying regions of very high G+C content. Nucl Acids Res 1993, 21: 2953–2954
Ehrlich GD, Greenberg SJ (eds.). PCR-based diagnostics in infectious disease. Blackwell Scientific Publications, Cambridge, Oxford, 1994 (ISBN 0–86542–0–86542)
Finckh U, Sander T, Rommelspacher H, Schmidt LG, Rolfs A. Allele-specific PCR for simultaneous amplification of both alleles of a deletion polymorphism in intron 6 of the human dopamine 2 receptor gene (DRD2). DNA Sequence 1996, 6: 87–94
Finckh U, Seeman P, von Widdern 0, Rolfs A. Simple PCR amplification of the entire glucocerebrosidase gene (GBA) coding region for diagnostic sequence analysis. DNA Sequence, 1998, 8: 349–356
Griffin HG, Griffin AM (eds.). PCR technology - current innovations. CRC Press, Boca Raton, Ann Arbor, 1994 (ISBN 0–8493–0–8493)
Holodniy M, Kim S, Katzenstein D, Konrad M, Groves E, Merigan TC. Inhibition of human immunodeficiency virus gene amplification by heparin. J Clin Microbiol 1991, 29: 676–679
Kalinina O, Lebedeva I, Brown J, Silver J. Nanoliter scale PCR with TaqMan detection. Nucleic Acids Res 1997, 25: 1999–2004
Lassner D, Pustowoit B, Rolfs A (eds.). Modern applications of DNA amplification tech-niques - problems and new tools. Plenum Press, New York, 1997 (ISBN 0–306–0–306)
McPherson MJ, Hames BD, Taylor GR (eds.). PCR 2 - a practical approach. IRL Press,Oxford, New York, 1995 (ISBN 0–19–0–19)
Miller SA, Dykes DD, Polesky HF. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 1988, 16: 1215
Morris T, Robertson B, Gallagher M. Rapid reverse transcription-PCR detection of hepatitis C virus RNA in serum by using the TaqMan fluorogenic detection system. J Clin Microbiol 1996, 34: 2833–2936
Mullis KB, Ferré F, Gibbs RA (eds.). The polymerase chain reaction. Birkhäuser, Boston, Basel, Berlin. 1994 (ISBN 0–8176–0–8176)
Newton CR (ed.). PCR - essential data. Wiley Press, Chichester, 1995 (ISBN 0–47195222–2)
Nuovo GJ. PCRin situhybridization. Raven Press, New York, 1994, 2nd edition (ISBN 0–7817–0183-X)
Persing DH (ed.). PCR protocols fo emerging infectious diseases. A supplement to: Diagnostic molecular microbioloy: principles and applications. ASM Press, Washington, 1996 (ISBN 1–55581–1–55581)
Rolfs A, Schuller I, Finckh U, Weber-Rolfs I. PCR: clinical diagnostics and research. Springer, Berlin, Heidelberg, 1992
Rolfs A, Weber-Rolfs I, Finckh U (eds.). Methods in DNA amplification. Plenum Press, New York, 1994 (ISBN 0–306–0–306)
Saiki RK, Scharf S, Fallona F, Mullis KB, Horn GT, Erlich HA, Arnheim N. Enzymatic amplication of ß-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 1995, 230: 487–491
Sarkar G (ed.). PCR in neuroscience. Methods in neuroscience, PM Conn (Eds.), Vol. 26. Academic Press, San Diego, New York, 1995 (ISBN 0–12–0–12)
Schuchard M, Sarkar G, Ruesink T, Spelsberg T C. Two step “hot” PCR amplification of GC-rich avianc-mycsequences. BioTechniques 1993, 14: 390–394
Taylor AC. Titration of heparinase for removel of the PCR-inhibitory effect of heparin in DNA samples. Mol Ecol 1997, 6: 383–385
Vandevyver C, Motmans K, Raus J. Quantificaton of cytokine mRNA expression by RTPCR and electrochemiluminescence. Genome Research 1995, 5: 195–201
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Rolfs, A., Finckh, U., Bauer, P. (2000). Amplification of Nucleic Acids by Polymerase Chain Reaction: Overview on Principles and Applications. In: Kessler, C. (eds) Nonradioactive Analysis of Biomolecules. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57206-7_25
Download citation
DOI: https://doi.org/10.1007/978-3-642-57206-7_25
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-64601-3
Online ISBN: 978-3-642-57206-7
eBook Packages: Springer Book Archive