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Heat-labile uracil-DNA glycosylase from a psychrophilic marine bacterium

  • Chapter
Biotechnological Applications of Cold-Adapted Organisms

Abstract

Uracil DNA glycosylase (UDG, EC 3.2.2.3), also known as uracil-N glycosylase (UNG) is the first enzyme involved in the base repair pathway for removal of uracil from DNA. The base uracil is removed from mutagenic U/G mispairs resulting from the deamination of cytosine and from U:A pairs resulting from misincorporation of dUMP during DNA synthesis.1,2 A mutagenic U/G mismatch leads to a C → T transition mutation in the next round of DNA synthesis. Although uracil is not mutagenic when base-pairing with adenine, uracil in place of thymine in regulatory DNA sequences can disrupt the binding of specific proteins.3 Uracil-DNA glycosylases hydrolyse the N-glycosidic bond linking the base to the deoxyribose sugar, generating an abasic site, which is subsequently removed by an apurinic/apyrimidinic (AP)-endonuclease and a phosphodiesterase. The resultant gap is filled in by a DNA polymerase and sealed by a DNA ligase.3 Uracil-DNA glycosylase shows high selectivity for the excision of uracil from DNA; it removes related bases such as isodialuric acid, 5-hydroxyuracil and alloxan at rates some orders of magnitude lower than with uraci1.4 No activity has been detected against any normal DNA base, nor against uracil in RNA. UNG excises uracil from single-and double-stranded DNA; for double-stranded DNA a preference for certain sequence contexts was shown.5

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Author information

Authors and Affiliations

  1. Boehringer Mannheim GmbH, Nonnenwald 2, D-82377, Penzberg, Germany

    H. Sobek

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  1. H. Sobek
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Editors and Affiliations

  1. Institute of Microbiology, University of Innsbruck, Technikerstraße 25, A-6020, Innsbruck, Austria

    Rosa Margesin  & Franz Schinner  & 

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© 1999 Springer-Verlag Berlin Heidelberg

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Sobek, H. (1999). Heat-labile uracil-DNA glycosylase from a psychrophilic marine bacterium. In: Margesin, R., Schinner, F. (eds) Biotechnological Applications of Cold-Adapted Organisms. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-58607-1_6

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  • DOI: https://doi.org/10.1007/978-3-642-58607-1_6

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-63663-9

  • Online ISBN: 978-3-642-58607-1

  • eBook Packages: Springer Book Archive

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