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Abstract

A significant advantage in DNA fingerprinting technology was made in the early 1990s through the development of a novel polymerase chain reaction (PRC)-based strategy which involves the use of a single oligonucleotide primers of arbitrary sequence to amplifly random genomic DNA fragments (Welsh and McClelland 1990; Williams et al. 1990; Caetano-Anollés et al. 1991). This strategy, often referred to as arbitrarily primed PCR, allows detection of polymorphisms between individuals (or strains) as differences between the patterns of DNA fragments amplified from the different DNAs using a given primer.

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© 1997 Springer-Verlag Berlin Heidelberg

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Micheli, M.R., Bova, R. (1997). Overview. In: Micheli, M.R., Bova, R. (eds) Fingerprinting Methods Based on Arbitrarily Primed PCR. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60441-6_1

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  • DOI: https://doi.org/10.1007/978-3-642-60441-6_1

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-47812-3

  • Online ISBN: 978-3-642-60441-6

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