Abstract
The mean resting human platelet was specified to be 1 × 3.1 µm2 in dimension, 4–7.6µm3 in volume (Frojmovic and Milton 1982) and 10pg in weight (Iyengar et al. 1979). Carefully prepared platelet-rich plasma (PRP) collected from citrated blood after venipuncture contains discocytes. The number of discocytes with small pseudopodia (echinodiscocytes) does not exceed 10%. Contact activation during centrifugation is the most abundant reason for the presence of activated platelets in PRP. The prefixation of the whole blood with low concentrations of aldehyde before centrifugation is well recommended (Stockinger et al. 1969) to preserve the discoid shape of platelets for ultra-structural examination. Proven washing procedures (Patscheke 1981) or the preparation of gel filtered platelets — preconditions for investigations on platelets under physiological concentrations of divalentions — preserve the resting state of platelets (Figs, 1a, 2a, 3). Additives like adenosine diphosphate (ADP) scavengers (apyrase) or inhibitors of thromboxane A2 are used to prevent platelet activation.
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Morgenstern, E. (1997). Human Platelet Morphology/Ultrastructure. In: von Bruchhausen, F., Walter, U. (eds) Platelets and Their Factors. Handbook of Experimental Pharmacology, vol 126. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60639-7_2
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