Abstract
The insecticide/ascaricide dinitro-ortho-cresol (DNOC) was tested for mutagenicity in BASC or Muller-5 test with Drosophila melanogaster. The frequencies of induced sexlinked recessive lethals in premeiotic and postmeiotic germ cell stages of spermatogenesis were determined after exposure of adult wild-type males by oral application of DNOC. Sex-linked recessive lethals were scored in the F2 generation according to standard procedures.
The mutagenic activity of DNOC at concentrations of 0.25 to 1.01 mM (50–200 ppm) in postmeiotic germ cell stages (11 lethals/1570 chromosomes tested = 0.70%) > premeiotic stages (3/1001 = 0.29%). The mutations rate in all germ cell stages was 0.59% (18/3140). The lowest effective concentration for the induction of recessive lethals in nature sperm was 0.25 mM which is 1/3 to ½ LD50 which is approximately 0.61 mM. The frequency of spontaneously occuring lethality was 0.06 (1/1718). The data presented here show significant differences in Kastenbaum-Bowman test.
Dimethylnitrosamine (6.8 mM = 500 ppm) induced a rate of 21.0% of lethals (112/534).
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Müller, J., Haberzettl, R. (1980). Mutagenicity of DNOC in Drosophila Melanogaster. In: Chambers, P.L., Klinger, W. (eds) Further Studies in the Assessment of Toxic Actions. Archives of Toxicology, vol 4. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67729-8_12
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DOI: https://doi.org/10.1007/978-3-642-67729-8_12
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