Abstract
Levine produced encephalitis in Lewis rats after injection of pertussis vaccine together with spinal cord tissue of guinea-pigs.
This animal model was used as an assay for the possible side-effects of pertussis vaccines prepared from whole bacteria or with extracted antigens.
Wistar and Lewis rats were injected with a mixture of guinea-pig spinal cord and cFA together with vaccines into the pad of each hind food. During a period of 25 days, the rats were observed for paralysis, ataxia, and death. Wistar rats were not found to be sensitive enough. Lewis rats were high susceptible in this model; they developed a high rate of allergic encephalopathy. DPT-vaccines prepared with soluble antigens showed a reduced neurotoxic activity.
The results were compared with the histamine-sensitizing assay and the mouse weight-gain test. In these assays similar results were found. The proposed animal assay is recommended in the preclinical testing of pertussis vaccines.
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References
Brouwer, R., Cohen, H.: Reactogenicity for infants of pertussis vaccines, harvested by centrifugation or acid precipitation. J. biol. Standard 4, 5–12 (1976)
Levine, S.: Hyperacute, neutrophilic, and localized forms of experimental allergic encephalomyelitis: a review. Acta neuropath. 28, 179–189 (1974)
Ström, J.: Reactions of a cerebral nature in conjunction with triple vaccination in Sweden, International Symp. on Pertussis, Symp. Series Immunobiol. Standard, Vol. 13, 157–160. Basel-München-New York: Karger 1970
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Ronneberger, H., Zwisler, O. (1980). Allergic Encephalomyelitis in Rats — Toxicity Assay for Pertussis Vaccines. In: Chambers, P.L., Klinger, W. (eds) Further Studies in the Assessment of Toxic Actions. Archives of Toxicology, vol 4. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67729-8_40
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DOI: https://doi.org/10.1007/978-3-642-67729-8_40
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