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Hormonal Regulation of Growth and Function of Insulin-Producing Cells in Culture

  • Conference paper
Hormonally Defined Media

Part of the book series: Proceedings in Life Sciences ((LIFE SCIENCES))

Abstract

The endocrine pancreas in mammals is organized in numerous small clusters of cells, the islets of Langerhans, consisting of 1000–3000 cells of which the insulin-producing beta-cells amount to about 60–70%. The islets represent only 1–2% of the total pancreas, and it was not before the introduction of the collagenase digestion technique (Moskalewski, 1965) that larger quantities of isolated pancreatic islets could be obtained for biochemical studies (for a review, see Nielsen and Lernmark, 1983).

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References

  • Andersson A (1975) Synthesis of DNA in isolated pancreatic islets maintained in tissue culture. Endocrinology 96: 1051–1054.

    Article  CAS  Google Scholar 

  • Andersson A (1978) Isolated mouse pancreatic islets in culture: Effects of serum and different culture media on the insulin production of the islets. Diabetologia 14: 397–404.

    Article  PubMed  CAS  Google Scholar 

  • Andersson A, Hellerstrom C (1972) Metabolic characteristics of isolated pancreatic islets in tissue culture. Diabetes 21 (suppl. 2): 546–554.

    PubMed  CAS  Google Scholar 

  • Billaudel B, Sutter BCJ (1981) Modulation of the direct effect of corticosteroid upon glucose-induced insulin secretion of rat isolated islets of Langerhans. Diab. Metab. 7: 91–96.

    CAS  Google Scholar 

  • Borelli MI, Garcia ME, Durum CLG, Gagliardino JJ (1982) Glucocorticoid- induced changes in insulin secretion related to the metabolism and ultrastructure of pancreatic islets. Horm. Metab. Res. 14: 287–292.

    Article  PubMed  CAS  Google Scholar 

  • Borg LAH (1982) Effects of steroid hormones on intracellular insulin degradation in isolated islets of Langerhans of the mouse. Diabetologia 23: 157.

    Google Scholar 

  • Brunstedt J (1980) Rapid isolation of functionally intact pancreatic islets from mice and rats by Percoll TMgradient centrifugation. Diab. Metab. 6: 87–89.

    CAS  Google Scholar 

  • Brunstedt J (1982) Expression of the insulin gene: Regulation by glucose, hydrocortisone, and growth hormone. Special FEBS Meeting on Cell Function and Differentiation, April 25–29, 1982 Athens, Greece, Abstracts p. 149.

    Google Scholar 

  • Brunstedt J, Nielsen JH (1981) Direct long-term effect of hydrocortisone on insulin and glucagon release from mouse pancreatic islets in tissue culture. Acta Endocr. (Cph) 96: 498–504.

    CAS  Google Scholar 

  • Chick WL (1973) Beta cell replication in rat pancreatic monolayer cultures. Effects of glucose, tolbutamide, glucocorticoid, growth hormone and glucagon. Diabetes 22: 687–693.

    PubMed  CAS  Google Scholar 

  • Fong HKW, Chick WL, Sato G (1981) Hormones and factors that stimulate growth of a rat islet tumor cell line in serum-free medium. Diabetes 30: 1022–1028.

    Article  PubMed  CAS  Google Scholar 

  • Green IC, Taylor KW (1972) Effects of pregnancy in the rat on the size and insulin secretory response of the islets of Langerhans. J. Endocrinol. 54: 317–325.

    Article  PubMed  CAS  Google Scholar 

  • Heding L (1971) Radioimmunological determination of pancreatic and gut glucagon in plasma. Diabetologia 7: 10–19.

    Article  PubMed  CAS  Google Scholar 

  • Hellerstrom C (1963) Effects of steroid diabetes on the pancreatic islets of guinea pigs with special reference to the A, cells. Acta Soc. Med. Upsal. 68: 1–16.

    Google Scholar 

  • Kern H, Logothetopoulos J (1970) Steroid diabetes in the guinea pig. Diabetes 19: 145–154.

    PubMed  CAS  Google Scholar 

  • King DL, Chick WL (1976) Pancreatic beta cell replication: Effects of hexose sugars. Endocrinology 99: 1003–1009.

    Article  PubMed  CAS  Google Scholar 

  • Logothetopoulos J (1972) Islet cell regeneration and neogenesis. In: Steiner DF, Freinkel H (eds.) Handbook of Physiology, Sect. 7, vol. 1. American Physiological Society, Washington DC, pp. 67–76.

    Google Scholar 

  • Moskalewski S (1965) Isolation and culture of the islets of Langer¬hans of the guinea pig. Gen. Comp. Endocr. 5: 342–353.

    Article  Google Scholar 

  • Nielsen JH (1981) Beta cell function in isolated human pancreatic islets in long-term tissue culture. Acta Biol. Med. Germ. 40: 55–60.

    PubMed  CAS  Google Scholar 

  • Nielsen JH (1982) Effects of growth hormone, prolactin, and placental lactogen on insulin content and release and deoxyribonucleic acid synthesis in cultured pancreatic islets. Endocrinology 110: 600–606.

    Article  PubMed  CAS  Google Scholar 

  • Nielsen JH, Brunstedt K (1982) Growth hormone as a growth factor for normal pancreatic islet cells in primary culture. In: Sirbasku DA, Sato G (eds.): Growth of cells in hormonally defined media. Cold Spring Habor Conference on -Cell Proliferation vol. 9 (in press).

    Google Scholar 

  • Nielsen JH, Lernmark A (1983) Purification of islets and cells from islets. In: Pretlow TG and Pretlow T (eds.) Cell Separation: Methods and selected applications. Academic Press, New York, vol. 2 (in press).

    Google Scholar 

  • Nielsen JH, Brunstedt J, Andersson A, Frimodt-M0ller C (1979) Preservation of beta cell function in adult human pancreatic islets for several months in vitro. Diabetologia 16: 97–100.

    Article  PubMed  CAS  Google Scholar 

  • Nielsen JH, J0rgensen R, Jensen D, Brunstedt K (1981) Direct effect of pregnancy hormones on growth and function of pancreatic islets in tissue culture. Diabetologia 21: 308–309.

    Article  Google Scholar 

  • Pierluissi J, Pierluissi R, Ashcroft SJH (1982) Effects of hypophysectomy and growth hormone on cultured islets of Langerhans of the rat. Diabetologia 22: 134–137.

    PubMed  CAS  Google Scholar 

  • Rabinovitch A, Blondel B, Murray T, Mintz DH (1980) Cyclic adenosine-351-monophosphate stimulates islet B cell replication in neonatal pancreatic monolayer cultures. J. Clin. Invest. 66: 1065–1071.

    Article  PubMed  CAS  Google Scholar 

  • Rabinovitch A, Quigley C, Russell T, Patel Y, Mintz DH (1982) Insulin and multiplication stimulating activity (an insulin-like growth factor) stimulate islet β-cell replication in neonatal rat pancreatic monolayer cultures. Diabetes 31: 160–164.

    Article  PubMed  CAS  Google Scholar 

  • Rabinovitch A, Quigley C, Rechler MM (1983) Growth hormone stimulates β-cell replication in neonatal rat pancreatic monolayer cultures. Diabetes (in press).

    Google Scholar 

  • Swenne I, Hellerstrom C (1982) Pancreatic β-cell regeneration in rats of various ages. Diabetologia 23: 203.

    Article  Google Scholar 

  • Swenne I, Bone AJ, Howell SL, Hellerstrom C (1980) Effects of glucose and amino acids on the biosynthesis of DNA and insulin in fetal rat islets maintained in tissue culture. Diabetes 29: 686–692.

    PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Hagedorn Research Laboratory, DK-2820, Gentofte, Denmark

    J. Høiriis Nielsen

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  1. J. Høiriis Nielsen
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Editor information

Editors and Affiliations

  1. Institut für Neurobiologie, Universität Heidelberg, Im Neuenheimer Feld 504, 6900, Heidelberg 1, Germany

    G. Fischer

  2. Institut für Pharmakologie, Universität Mainz, Obere Zahlbacher Str. 67, 6500, Mainz, Germany

    R. J. Wieser

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© 1983 Springer-Verlag Berlin Heidelberg

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Nielsen, J.H. (1983). Hormonal Regulation of Growth and Function of Insulin-Producing Cells in Culture. In: Fischer, G., Wieser, R.J. (eds) Hormonally Defined Media. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69290-1_31

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  • DOI: https://doi.org/10.1007/978-3-642-69290-1_31

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-69292-5

  • Online ISBN: 978-3-642-69290-1

  • eBook Packages: Springer Book Archive

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