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Bronchoalveolar Lavage

  • Chapter
Toxicology of Inhaled Materials

Part of the book series: Handbook of Experimental Pharmacology ((HEP,volume 75))

Abstract

After filling all or part of the lungs with saline via the airways, the fluid can be withdrawn. The recovered saline contains both cells and molecules harvested from airway and alveolar lining fluids. Thus, bronchoalveolar lavage (BAL) is a way to biopsy the extensive surfaces of the respiratory tract. This is both an advantage and a limitation. Sampling problems, so often characteristic of light and electron microscopy, do not occur since all of the surface in a lobe or lung is sampled. The average response of the entire lung can easily be described. On the other hand, as with many pulmonary function tests such as FEV1, there is little or no information regarding the particular area injured. One should also note that BAL is a convenient way to recover macrophages and other cells for further in vitro studies. Thus, BAL is often a first step to studies of in vitro phagocytosis, chemotaxis, or mediator release. As will be evident from the rest of this chapter, there is little doubt that BAL has joined the armamentarium of analytic techniques available to the inhalation toxicologist.

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Authors
  1. J. D. Brain
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  2. Barbara D. Beck
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Editors and Affiliations

  1. Toxicology, Biology Division, Oak Ridge National Laboratory, P.O. Box Y, 37831, Oak Ridge, TN, USA

    Hanspeter Witschi M.D. (Section Head) (Section Head)

  2. Department of Environmental Science and Physiology, Harvard University School of Public Health, 665 Huntington Ave., 02115, Boston, MA, USA

    Joseph D. Brain S.D. in Hyg. (Professor of Physiology, Director, Respiratory Biology Program) (Professor of Physiology, Director, Respiratory Biology Program)

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© 1985 Springer-Verlag Berlin Heidelberg

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Brain, J.D., Beck, B.D. (1985). Bronchoalveolar Lavage. In: Witschi, H., Brain, J.D. (eds) Toxicology of Inhaled Materials. Handbook of Experimental Pharmacology, vol 75. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69521-6_8

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  • DOI: https://doi.org/10.1007/978-3-642-69521-6_8

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-69523-0

  • Online ISBN: 978-3-642-69521-6

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