Abstract
Human adenoviruses, especially types 2, 5, and 12, have been the subject of extensive studies because of their ability to cause morphological transformation of cultured cells or, in the case of Ad12, to induce tumors in animals. On the other hand, the adenovirus/cell system has clearly established itself as a useful tool for studying gene expression in mammalian cells. Several precise chemical tools for studying the adenovirus genome have become available in recent years; these include methods for separating restriction enzyme fragments of viral DNA and hybridization methods for detecting viral RNAs. The application of recombinant DNA technology has yielded precise information about the genomic and the messenger RNA sequences expressed by different regions of the genome. Most but not all of the proteins encoded by the adenovirus genome have been characterized by structure, but the function of the proteins is still unresolved.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Achten S, Doerfler W (1982) Virus-specific proteins in adenovirus type 12-transformed and tumor cells as detected by immunoprecipitation. J Gen Virol 59:357–366
Anderson CW, Lewis JB, Atkins JF, Gesteland RF (1974) Cell-free synthesis of adenovirus 2 proteins programmed by fractioned messenger RNA : a comparison of polypeptide products and messenger RNA lengths. Proc Natl Acad Sci USA 71:2756–2760
Berget AM, Sharp PA (1979) Structure of late adenovirus 2 heterogenous nuclear RNA. J Mol Biol 129:547–565
Berk AJ, Lee F, Harrison T, Williams J, Sharp PA (1979) Preearly adenovirus 5 gene product regulates synthesis of early viral messenger RNAs. Cell 17:1935–1944
Bos JL, Polder LJ, Bernards R, Schrier PI, van der Elsen PJ, van der Eb AJ, van Ormondt H (1981) The 2.2 kb E1b mRNA of human Adl2 and Ad5 codes for two tumor antigens starting at different AUG triplets. Cell 27:121–131
Cepko CL, Chanelian PS, Sharp PH (1982) Immunoprecipitation with two-dimensional pools as a hybridoma screening technique: production and characterization of monoclonal antibodies against adenovirus 2 proteins. Virology 11:385–401
Challberg M, Kelly TS (1979) Adenovirus DNA replication in vitro. Proc Natl Acad Sci USA 76:655–659
Chow LT, Roberts JM, Lewis JB, Broker TR (1977) A map of cytoplasmic RNA transcripts from lytic adenovirus type 2, determined by electron microscopy of RNA:DNA hybrids. Cell 11:819–836
Chow LT, Broker TR, Lewis JB (1979) Complex splicing patterns of RNAs from the early regions of adenovirus 2. J Mol Biol 14:265–304
Craig EA, Raskas HJ (1974) Effect of cycloheximide on RNA metabolism early in productive infection with adenovirus 2. J Virol 14:26–32
Doerfler W (1968) The fate of DNA of adenovirus type 12 in baby hamster kidney cells. Proc Natl Acad Sci USA 60:636–643
Doerfler W (1969) Nonproductive infection of baby hamster kidney cells (BHK21) with adenovirus type 12. Virology 38:587–606
Doerfler W (1970) Integration of DNA of adenovirus type 12 into the DNA of baby hamster kidney cells. J Virol 6:652–666
Doerfler W (1975) Integration of viral DNA into the host genome. In: Current Topics in Microbiology and Immunology Vol 71. Springer, Berlin Heidelberg New York, pp 1–78
Doerfler W (1977) Animal virus-host genome interactions. In: Fraenkel-Conrat H, Wagner RR (eds) Comprehensive virology. Plenum, New York, vol 10, pp 279–399
Doerfler W (1981) DNA methylation-a regulatory signal in eucaryotic gene expression. J Gen Virol 57:1–20
Doerfler W (1982) Uptake, fixation and expression of foreign DNA in mammalian cells: the organization of integrated adenovirus DNA sequences. In: Graf T, Jaenisch R (eds) Tumorviruses, neoplastic transformation and differentiation. Springer, Berlin Heidelberg New York, pp 127–194 (Current topics in microbiology and immunology, vol 101)
Doerfler W (1983) DNA methylation and gene activity. Annu Rev Biochem 52:1–20
Doerfler W, Lundholm U (1970) Absence of replication of the DNA of adenovirus type 12 cells. Virology 40:754–757
Doerfler W, Lundholm U, Hirsch-Kaufmann M (1972) Intracellular forms of adenovirus deoxyribonucleic acid. I. Evidence for a deoxyribonucleic acid-protein complex in baby hamster kidney cells infected with adenovirus type 12. J Virol 9:297–308
Doerfler W, Burger H, Ortin J, Fanning E, Brown DT, Westphal M, Winterhoff U, Weiser B, Schick J (1974) Integration of adenovirus DNA into the cellular genome. Cold Spring Harbor Symp Quant Biol 39:505–521
Doerfler W, Stabel S, Ibelgaufts H, Sutter D, Neumann R, Groneberg J, Scheidtmann KH, Deuring R, Winterhoff U (1979) Selectivity in integration sites of adenoviral DNA. Cold Spring Harbor Symp Quant Biol 44:551–564
Eggerding F, Raskas HJ (1978) Effect of protein synthesis inhibitors on viral RNAs synthesized early in adenovirus type 2 infection. J Virol 25:453–458
Esche H (1982) Viral gene products in adenovirus type 2-transformed hamster cells. J Virol 41:1076–1082
Esche H, Bause E (1984) Antibodies specific for amino-and carboxy-terminal regions of adenovirus type 12 tumor antigens. Virus Research (submitted)
Esche H, Siegmann B (1982) Expression of early viral gene products in adenovirus type 12-infected and-transformed cells. J Gen Virol 60:99–113
Esche H, Schilling R, Doerfler W (1979) In vitro translation of adenovirus type 12-specific mRNA isolated and transformed cells. J Virol 30:21–31
Evans RM, Fraser N, Ziff E, Weber J, Wilson M, Darnell JE (1977) The initiator sites for RNA transcription in Ad2 DNA. Cell 12:733–739
Fanning E, Doerfler W (1976) Intracellular forms of adenovirus DNA. V. Viral DNA sequences in hamster cells abortively infected and transformed with human adenovirus type 12. J Virol 20:373–383
Flint SJ, Gallimore PH, Sharp PH (1975) Comparison of viral RNA sequences in adenovirus-transformed and lytically infected cells. J Mol Biol 96:47–68
Flint SJ, Sambrook J, Williams JF, Sharp PH (1976) Viral nucleic acid sequence in transformed cells. IV. A study of the sequences of adenovirus 5 DNA and RNA in four lines of adenovirus 5-transformed rodent cells using specific fragments of the viral genome. Virology 72:456–470
Gallimore PH, Sharp PA, Sambrook J (1974) Viral DNA in transformed cells. II. A study of the sequences of adenovirus 2 DNA in nine lines of transformed rat cells using specific fragments of the viral genome. J Mol Biol 89:49–72
Ginsberg HS, Bello LJ, Levine AJ (1967) Control of biosynthesis of host macromolecules in cells infected with adenoviruses. In: Colter JS, Paranchych W (eds) The molecular biology of adenoviruses. Academic, New York, pp 547–573
Ginsberg HS, Ensinger MS, Kauffman RS, Mayer AJ, Londholm U (1974) Cell transformation: a study of regulation with types 5 and 12 adenovirus temperature-sensitive mutants. Cold Spring Harbor Symp Quant Biol 39:412–426
Graham FL, Abrahams PJ, Mulder C, Heijneker HL, Wamaar SO, de Vries FAJ, Fiers W, van der Eb AJ (1974) Studies on in vitro transformation by DNA fragments of human adenoviruses and simian virus 40. Cold Spring Harbor Symp Quant Biol 39:637–650
Graham FL, Harrison T, Williams J (1978) Defective transforming capacity of adenovirus type 5 host-range mutants. Virology 86:10–21
Green M (1970) Oncogenic viruses. Annu Rev Biochem 39:701–756
Green M, Parson TJ, Pina M, Fujinaga K, Caffier H, Landgraf-Leurs IM (1970) Transcription of adenovirus genes in productively infected and in transformed cells. Cold Spring Harbor Symp Quant Biol 35:803–818
Green M, Pina M, Kimes R, Wensink PC, Mac Hattie LA, Thomas CA (1967) Adenovirus DNA I. Molecular weight and conformation. Proc Natl Acad Sci USA 57:1302–1311
Horwitz MS (1978) Temperature sensitive replication of H5ts125 adenovirus DNA in vitro. Proc Natl Acad Sci USA 75:4291–4295
Huebner RJ, Rowe WP, Turner HC, Lane WT (1963) Specific adenovirus complement-fixing antigens in virus-free hamster and rat tumors. Proc Natl Acad Sci USA 50:379–389
Jochemsen H, Daniels GSG, Kupker H, van der Eb AJ (1980) Identification and mapping of early gene products of adenovirus type 12. Virology 105:172–188
Jochemsen H, Daniels GSG, Hertoghs JJL, Schrier PI, van den Elson PJ, van der Eb AJ (1982) Identification of adenovirus type 12 gene products involved in transformation and oncogenesis. Virology 122:15–28
Jones N, Shenk T (1978) Isolation of deletion and substitution mutants of adenovirus type 5. Cell 13:181–197
Jones N, Shenk T (1979) An adenovirus type 5 early gene function regulates expression of other early viral genes. Proc Natl Acad Sci USA 76:3665–3669
Jung YH, Wold WSM, Sugawara K, Green M (1978) Evidence for an adenovirus type 2 coded early glycoprotein. J Virol 28:314–323
Kuhlmann I, Achten S, Rudolph R, Doerfler W (1982) Tumor induction by human adenovirus type 12 in hamsters: loss of the viral genome from adenovirus type 12-induced tumor cells is compatible with tumor formation. EMBO J 1:79–86
Levine AJ (1984) The adenovirus early proteins. In: Doerfler W (ed) The molecular biology of adenoviruses I. Springer, Berlin Heidelberg New York (Current topics in microbiology and immunology, vol 109)
Levinson AD, Levine AL (1977) The group C adenovirus tumor antigens: identification in infected and transformed cells and a peptide map analysis. Cell 1:871–879
Lewis JB, Esche H, Smart JE, Stillman B, Harter ML, Mathews MB (1979) Organization and expression of the left third of the genome of adenovirus. Cold Spring Harbor Symp Quant Biol 44:493–508
Lichy JH, Field J, Horwitz M (1982) Seperation of the adenovirus terminal protein precursor from its associated DNA polymerase : role of both proteins in the initiation of adenovirus DNA replication. Proc Natl Acad Sci USA 79:5225–5229
Manley JL, Sharp PA, Gefter M (1979) RNA synthesis in isolated nuclei: in vitro mutation of adenovirus 2 major late mRNA precursor. Proc Natl Acad Sci USA 76:160–164
Nevins J (1982) Induction of the synthesis of a 70,000 dalton mammalian heat shock protein by the adenovirus E1A gene product. Cell 29:913–919
Nevins J, Darnall JE (1978) Groups of adenovirus type 2 mRNAs derived from large primary transcript: probably nuclear origin and possible common 3´-ends. J Virol 25:811–823
Ortin J, Doerfler W (1975) Transcription of the genome of adenovirus type 12. I. Viral mRNA in abortively infected and transformed cells. J Virol 15:27–35
Ortin J, Scheidtmann KH, Greenberg R, Westphal M, Doerfler W (1976) Transcription of the genome of adenovirus type 12. III. Maps of stable RNA from productively infected human cells and abortively infected and transformed hamster cells. J Virol 20:355–372
Persson H, Kvist S, Ostberg L, Petersson PA, Philipson L (1979) Adenoviral early glycoprotein E3–191X and its association with transplantation antigens. Cold Spring Harbor Symp Quant Biol 44:509–517
Pettersson U, Mathews MB (1977) The gene and messenger RNA for adenovirus polypeptide IX. Cell 12:741–750
Philipson L, Pettersson U, Lindberg U (1975) Molecular biology of adenoviruses. Virology 14:1–115
Raska K, Strohl WA (1972) The response of BHK21 cells to infection with type 12 adenovirus. VI. Synthesis of Virus-specific RNA. Virology 47:734–742
Reuther-Brötz M (1981) Identifikation und Kartierung der späten Proteine von Adenovirus Typ 12. Thesis, University of Cologne, Cologne, Germany
Reuther M, Esche H (1984, to be published) The map of adenovirus type 12 late proteins. Virology
Rosenwirth B, Shiroki K, Levine AJ, Shimojo H (1975) Isolation and characterization of adenovirus type 12 DNA binding proteins. Virology 67:14–23
Russel WC, Hayashi K, Sanderso PJ, Pereira HG (1967) Adenovirus antigens: a study of their properties and sequential development in infection. J Virol 1:495–507
Saito I, Shiroki K, Shimojo H (1983) Messenger RNA species and proteins of adenovirus type 12 transforming regions: identification of proteins translated from multiple coding stretches in 2.2 kb region 1B mRNA in vitro and in vivo. Virology 127:271–289
Sambrook J, Botchan M, Gallimore P, Ozanne B, Pettersson U, Sharp WJ (1974) Viral DNA sequences in cells transformed by simian virus 40, adenovirus type 2 and adenovirus type 5. Cold Spring Harbor Symp Quant Biol 39:615–632
Sarnow P, Sullivan CA, Levine AJ (1982) A monoclonal antibody detecting the adenovirus 5 E1b-58kd tumor antigen: characterization of the Elb-58kd tumor antigen in adenovirus infected and transformed cells. Virology 34:650–657
Sawada Y, Fujinaga K (1980) Mapping of adenovirus 12 mRNAs transcribed from the transforming region. J Virol 36:639–651
Scheidtmann KH, Ortin J, Doerfler W (1975) Transcription of the genome of adenovirus type 12. II. Viral mRNA in productively infected KB cells. Eur J Biochem 58:283–290
Schirm S, Doerfler W (1981) Expression of viral DNA in adenovirus type 12-transformed cells, in tumor cells, and in revertants. J Virol 39:694–702
Schrier PI, Bernards R, Vaessen RTMJ, Houweling A, van der Eb AJ (1984, to be published) Highly oncogenic adenovirus 12 switches of the expression of class I major histocompility antigens in transformed rat cells. J Immunol
Sekikawa K, Shiroki K, Shimojo H, Ojima S, Fujinaga K (1978) Transformation of a rat cell line by an adenovirus 7 DNA fragment. Virology 88:1–7
Sharp PA, Gallimore PH, Flint SJ (1974) Mapping of adenovirus 2 RNA sequences in lytically infected cells and transformed cell lines. Cold Spring Harbor Symp Quant Biol 39:457–474
Shenk T, Jones N, Colby W, Fowlkes D (1979) Functional analysis of Ad5 host range deletion mutants defective for transformation of rat embryo cells. Cold Spring Harbor Symp Quant Biol 44:367–375
Shimojo H, Shiroki K, Yamaguchi K (1974) The viral DNA replication machinery of adenovirus 12. Cold Spring Harbor Symp Quant Biol 39:533–538
Shiroki K, Handa H, Shimojo H, Yano H, Ojima S, Fujinaga K (1977) Establishment and characterization of rat cell lines transformed by restriction endonuclease fragments of adenovirus 12 DNA. Virology 82:462–471
Stabel S, Doerfler W, Friis RR (1980) Integration sites of adenovirus type 12 DNA in transformed hamster cells and hamster tumor cells. J Virol 36:22–40
Starzinski-Powitz A, Schulz M, Esche H, Mukai N, Doerfler W (1982) The adenovirus type 12-mouse cell system: permissivity and analysis of integration patterns of viral DNA in tumor cells. EMBOJ 1:493–491
Stillman B (1981) Adenovirus DNA-replication in vitro: A protein linked to the 5´ end of nascent DNA strands. J Virol 37:139–147
Stillman B, Lewis JB, Chow LT, Mathews MB, Smart JE (1981) Identification of the gene and mRNA for the adenovirus terminal protein precursor. Cell 23:497–508
Strohl WA (1969) The response of BHK21 cells to infection with type 12 adenovirus. I. Cell killing and T antigen synthesis as correlated viral genome functions. Virology 39:642–652
Sugisaki H, Sugimoto K, Takanami M, Shiroki K, Saito I, Shimojo H, Sawada Y, Uemizu Y, Uesugi SI, Fujinaga K (1980) Structure and gene organization in the transforming HindIII-G fragment of Adl2. Cell 20:777–786
Sutter D, Westphal M, Doerfler W (1978) Pattern of integration of viral DNA sequences in the genomes of adenovirus type 12-transformed hamster cells. Cell 14:569–585
Tooze J (ed) (1981) Molecular biology of tumor viruses. 2nd ed, part 2. DNA tumor viruses. Cold Spring Harbor, New York
van den Elsen PJ, de Pater S, Houwelinh A, van der Veer J, van der Eb AJ (1981) The relationship between region E1A and E1B of human adenoviruses in cell transformation. Gene 18:175–185
van den Elsen PJ, Houweling A, van der Eb AJ (1983) Expression of region E1B of human adenoviruses in the absence of a functional E1A region is not sufficient for complete transformation. Virology
van der Eb AJ, Mulder C, Graham FL, Houweling A (1977) Transformation with specific fragments with transforming activity of adenovirus 2 and 5 DNA. Gene 2:115–132
van der Eb AJ, van Ormondt H, Schrier PI, Lupker JH, Jochemsen H, van den Elsen PJ, De Leys RJ, Kratt J, van Beveren CP, Dykema R, De Wood A (1979) Structure and function of the transforming genes of human adenoviruses and SV40. Cold Spring Harbor Symp Quant Biol 44:383–399
van der Vliet PC, Levine AJ (1973) DNA-binding proteins specific for cells infected by adenovirus. Nature 246:170–174
van der Vliet PC, Sussenbach JS (1975) An adenovirus type 5 gene function required for initiation of viral DNA replication. Virology 67:415–427
van der Vliet PC, Levine AJ, Ensinger MS, Ginsberg HS (1975) Thermolabile DNA binding proteins from cells infected with a temperature-sensitive mutant of adenovirus defective in viral DNA synthesis. J Virol 15:348–354
Vardimon L, Renz D, Doerfler W (1983) Can DNA methylation regulate gene expression? Cancer Res 84:90–102
Wadell G (1979) Classification of human adenoviruses by SDS-polyacrylamide gel electrophoresis of structural polypeptides. Intervirology 11:47–57
Weber J, Mak S (1972) Synthesis of viral components in hybrids of differentially permissive cells infected with adenovirus type 12. Exp Cell Res 74:423–429
Wilson M, Fraser N, Darnell J (1979) Mapping of RNA initiation sites by high dose of UV irradiation. Evidence for three independent promoters within the left 11% of the Ad2-genome. Virology 94:175–184
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Esche, H., Reuther, M., Schughart, K. (1984). Early and Late Proteins of Adenovirus Type 12: Translation Mapping with RNA Isolated from Infected and Transformed Cells. In: Doerfler, W. (eds) The Molecular Biology of Adenoviruses 3. Current Topics in Microbiology and Immunology, vol 111. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69549-0_4
Download citation
DOI: https://doi.org/10.1007/978-3-642-69549-0_4
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-69551-3
Online ISBN: 978-3-642-69549-0
eBook Packages: Springer Book Archive