Summary
Cosmid gene libraries were established from two strains of Azospirillum lipoferum (ATCC 29708 and 29731). The clones carrying the nif structural genes (nifHDK) were isolated. These genes were found to be clustered and organized in the same order as in Klebsiella pneumoniae (nifHDK). After introduction into the respective K. pneumoniae nif - mutants the cloned A. lipoferum nifHDK (Ali nifHDK) restored nitrogenase activity up to approximately 2% of wildtype.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Ruvkun, G. B. and Ausubel, F. M. (1980). Proc. Natl. Acad. Sci. USA 77, 191–195
Elmerich, C. and Franche, C. (1982). In Azospirillum: Genetics, Physiology, Ecology. W. Klingmüller (Ed.) Birkhäuser Verlag, Basel, Experientia Supplementum 42, 9–17
Wenzel, W., Singh, M. and Klingmüller, W. (1983). In Azospirillum II: Genetics, Physiology, Ecology. W. Klingmüller (Ed.), Birkhäuser Verlag, Basel, Experientia Supplementum 48, 39–46
Quiviger, B., Franche, C., Lutfalla, G., Rice, D., Haselkorn,R. and Elmerich, C. (1982). Biochimie 64, 495–503
Fischer, H.-M. and Hennecke, H. (1984). Mol. Gen. Genet. 196, 537–540
Haselkorn, R., Golden, J., Robinson, S. J., Avtges, P., Kranz, R. G. and Scolnik, P. A. (1985). In Nitrogen fixation and C02 Metabolism. P.W. Ludden and J. E. Burris (Eds.), Elsevier Science Publishing Co., Inc., pp 83–90
Adams, T. H., McClung, C. R. and Chelm, B. K. (1984). J. Bacteriol. 159, 857–862
Tarrand, J. J., Krieg, N. and Döbereiner, J. (1978). Can. J. Microbiol. 24, 967–980
Martinez-Drets, G., Del Gallo, M., Burpee, C. and Burris, R. H. (1984). J. Bacteriol. 159, 80–85
Goebel, E.M. and Krieg, N. R. (1984). J. Bacteriol. 159, 86–92
Hartmann, A., Singh, M. and Klingmüller, W. (1983). Can. J. Microbiol. 29, 916–923
Hohn, B., (1979). In Methods in Enzymology 68. R. Wu (Ed.), Academic press, New York, London, Toronto, pp. 299–309.
Merrick, M., Filser, M., Kennedy, C. and Dixon, R. (1978). Mol. Gen. Genet. 165, 103–111
Dixon, R., Kennedy, C., Kondorosi, A., Krishnapillai, V. and Merrick, M. (1977). Mol. Gen. Genet. 157, 189–198
Riedel, G. E., Ausubel, F. M. and Cannon, F. C. (1979). Proc. Natl. Äcad. Sei. USA 76, 2866–2870
Cannon, F. C., Dixon, R. A., Postgate, J. R. and Primrose, S.B. (1974). Mol. Gen. Genet. 174, 59–66
Marmur, J. (1961). J. Mol. Biol. 3, 208–218
Rodriguez, R. L. and Tait, R. C. (1983). Recombinant DNA techniques. An introduction. Addison-Wesley publishing company, London
Singh, M. and Klingmüller, W. In: Trends in molecular genetics. U. Sinha and W. Klingmüller (Eds.) Spectrum Publishing House, Patna, Delhi, pp. 15–34, (in press)
Rigby, P.W.J., Dieckmann, M., Rhodes, C. and Berg, P. (1977). J. Mol. Biol. 113, 237–251
Singh, M. and Klingmüller, W. (1984). In Proceedings of the 3rd international symposium on nitrogen fixation with non- legumes, Helsinki, (in press)
Jones, R., Woodley, P. and Robson, R. (1984). Mol. Gen. Genet. 197, 318–327
Riedel, G. E., Brown, S. E. and Ausubel, F. M. (1983). J. Bacterid. 153, 45–56
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1985 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Fahsold, R., Singh, M., Klingmüller, W. (1985). Cosmid Cloning of Nitrogenase Structural Genes of Azospirillum Lipoferum. In: Klingmüller, W. (eds) Azospirillum III. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70791-9_4
Download citation
DOI: https://doi.org/10.1007/978-3-642-70791-9_4
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-70793-3
Online ISBN: 978-3-642-70791-9
eBook Packages: Springer Book Archive