Abstract
The demonstration of the genetically determined alpha-l-antitrypsine (PI) phenotypes may be achieved generally by isoelectrofocusing (IEF) on polyacrylamide gels (PAGIF) with carrier ampholytes. This method which originally was applied at a pH-gradient pH 3.5 — pH 6.does not produce a sufficient resolution quality though recently carrier ampholytes with a pH gradient pH 4.2 – 4.9 are offering an improved resolution (3).
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Ferstl, F., Hoppe, H.H. (1986). Alpha-l-Antitrypsine (Pi) Polymorphism. Improved Pi-M-Subtyping by PAGIF with Immobilized Gradients.. In: Brinkmann, B., Henningsen, K. (eds) 11th Congress of the Society for Forensic Haemogenetics (Gesellschaft für forensische Blutgruppenkunde e.V.). Advances in Forensic Haemogenetics, vol 1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71150-3_20
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DOI: https://doi.org/10.1007/978-3-642-71150-3_20
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