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Glutathione S-Transferase Subunits in the Mouse and Their Catalytic Activities Towards Reactive Electrophiles

  • Conference paper
Mouse Liver Tumors

Part of the book series: Archives of Toxicology ((TOXICOLOGY,volume 10))

Abstract

The glutathione S-transferases (GST) are a major, multi-gene, group of detoxication proteins. A rapid, two-step purification, that employs S-hex- ylglutathione affinity chromatography and hydroxyapatite HPLC, is described for the GST isoenzymes in mouse liver. The major hepatic forms comprise Yf(Mr 24500)-, Ya(Mr 26000)- and Yb(Mr 27000)-type subunits. The isoeletric points of the Yf, Ya and Yb dimers are 8.6, 9.2 and 7.8–8.2 respectively. Immunochemical experiments showed the purified mouse subunits cross-reacted with antisera raised against rat GST subunits that possessed equivalent molecular masses; the subunit sizes of the distinct subunit types are conserved between these two species. The catalytic activities of the purified mouse GST subunits are described. In common with other species the mouse GST are subject to tissue-specific expression. However, only mouse liver and not rat, guinea pig, hamster or human livers express the Yf polypeptide. The significance of this difference is unclear but, in the rat, the Yf subunit can serve as a marker for pre-neoplastic hepatocellular carcinogenesis.

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Author information

Authors and Affiliations

  1. Department of Clinical Chemistry, The Royal Infirmary, University of Edinburgh, Edinburgh, EH3 9YW, Scotland, UK

    J. D. Hayes, R. E. Coulthwaite, P. K. Stockman & A. J. Hussey

  2. Department of Biochemistry, Trinity College, Dublin 2, Ireland

    T. J. Mantle

  3. ICRF Laboratory of Molecular Pharmacology and Drug Metabolism, Department of Biochemistry, University of Edinburgh, Edinburgh, EH8 9XD, Scotland, UK

    C. R. Wolf

Authors
  1. J. D. Hayes
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  2. R. E. Coulthwaite
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  3. P. K. Stockman
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  4. A. J. Hussey
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  5. T. J. Mantle
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  6. C. R. Wolf
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Editor information

Editors and Affiliations

  1. Department of Pharmacology and Therapeutics, Trinity College, University of Dublin, Dublin 2, Ireland

    Philip L. Chambers

  2. Institut für Toxikologie und Pharmakologie, der Universität Würzburg, Versbacher Landstraße 9, 8700, Würzburg, Germany

    Dietrich Henschler

  3. Institut für Toxikologie, der Universität Mainz, Obere Zahlbacher Straße 67, 6500, Mainz, Germany

    Franz Oesch

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© 1987 Springer-Verlag

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Hayes, J.D., Coulthwaite, R.E., Stockman, P.K., Hussey, A.J., Mantle, T.J., Wolf, C.R. (1987). Glutathione S-Transferase Subunits in the Mouse and Their Catalytic Activities Towards Reactive Electrophiles. In: Chambers, P.L., Henschler, D., Oesch, F. (eds) Mouse Liver Tumors. Archives of Toxicology, vol 10. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71617-1_11

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  • DOI: https://doi.org/10.1007/978-3-642-71617-1_11

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-17124-9

  • Online ISBN: 978-3-642-71617-1

  • eBook Packages: Springer Book Archive

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