Abstract
The extraction procedure involves three consecutive extractions with phenol and three washings of the extracted RNA with 70 % ethanol. Several measures ensure high quality of the RNA:
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Working under conditions of high pH in which the binding between RNA and coat proteins is weakened
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Applying macaloid (a clay) to adsorb RNase
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Use of SDS to denature RNase
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Adding EDTA for binding oxidising ions, such as Fe3+, which may otherwise generate harmful oxygen radicals
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Use of redistilled phenol to avoid the presence of oxidised polyphenols
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Use of chloroform to remove fatty acids
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Use of isoamyl alcohol to avoid generation of foam due to the presence of SDS
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© 1998 Springer-Verlag Berlin Heidelberg
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Dijkstra, J., de Jager, C.P. (1998). RNA Extraction from Purified Virus Particles. In: Practical Plant Virology. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72030-7_49
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DOI: https://doi.org/10.1007/978-3-642-72030-7_49
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-48981-5
Online ISBN: 978-3-642-72030-7
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