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Cryopreservation of Potato Germplasm

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Potato

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 3))

Abstract

To maintain germplasm of potato, the ‘seeds’ (tubers) have to be grown and multiplied in nurseries every year. Numerous clones of potato are being maintained at the International Potato Centre, Lima by this method, which is not only time-consuming, labour-intensive, expensive and cumbersome, but above all, the material is exposed to the hazards of pests and diseases, and may result in the total loss of germplasm. Thus, the problem of the conservation of genetic resources of vegetatively propagated plants has encouraged attempts to develop unconventional methods. As an alternative, meristem cultures have been employed for storage (see Sects. V.2 and V.3, this Vol.). However, this method is effective for short-term maintenance as it involves transfer to fresh media and constant vigilance and control of storage conditions. At present, no method is available by which long-term conservation of germplasm of vegetatively propagated plants can be ensured.

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References

  • Bajaj YPS (1976) Regeneration of plants from cell suspensions frozen at -20, -70 and -196 C. Physiol Plant 37:263–268

    Article  Google Scholar 

  • Bajaj YPS (1977) Initiation of shoots and callus from potato tuber-sprouts and axillary buds frozen at -196 C. Crop Improv 4:48–53

    Google Scholar 

  • Bajaj YPS (1978) Tuberization in potato plants regenerated from freeze-preserved meristems. Crop Improv 5:137–141

    Google Scholar 

  • Bajaj YPS (1979 a) Long-term preservation of potatoes through freeze storage of meristem cultures. Proc Int Symp Post harvest technology -utilization of potatoes. CIP Simla New Delhi, pp 283–292

    Google Scholar 

  • Bajaj YPS (1979 b) Technology and prospects of cryopreservation of germplasm. Euphytica 28:267–285

    Article  Google Scholar 

  • Bajaj YPS (1979c) Establishment of germplasm banks through freeze storage of plant tissue cultures and their implications in agriculture. In: Sharp WR, Larsen PO, Paddock EF, Raghavan V (eds) Plant cell and tissue culture -principles and applications. Ohio State Univ Press, Columbus, pp 745–774

    Google Scholar 

  • Bajaj YPS (1981) Regeneration of plants from potato meristems freeze-preserved for 24 months. Euphytica 30:141–145

    Article  Google Scholar 

  • Bajaj YPS (1982) Cryopreservation of germplasm of potato, cassava, peanut and chickpea. In: Fujiwara A (ed) Plant tissue culture 1982. Maruzen, Tokyo, pp 799–800

    Google Scholar 

  • Bajaj YPS (1983 a) Cassava plants from meristem cultures freeze-preserved for three years. Field Crop Res 7:161–167

    Article  Google Scholar 

  • Bajaj YPS (1983 b) Production of normal seeds from plants regenerated from meristems of Arachis hypogaea and Cicer arietinum cryopreserved for 20 months. Euphytica 32:425–430

    Article  Google Scholar 

  • Bajaj YPS (1984 a) Cryoconservation of germplasm of crops -Present status and prospects. In: Novak FJ, Havel L, Doleiel J (eds) Int Symp Plant tissue and cell culture, application to crop improvement. Czech Acad Sci, Prague, pp 607–616

    Google Scholar 

  • Bajaj YPS (1984b) Biotechnology of the conservation of germplasm and its implications in agriculture and forestry. Proc Int Symp Genetic manipulation of crops. Acad Sin, Beijing

    Google Scholar 

  • Bajaj YPS (1985) Cryopreservation of germplasm of potato (Solarium tuberosum L.) and cassava (Manihot esculenta Crantz): Viability of excised meristems cryopreserved up to four years. Indian J Exp Biol 23:285–287

    Google Scholar 

  • Bajaj YPS (1986 a) In vitro preservation of genetic resources -Techniques and problems. Int Symp Nuclear techniques and in-vitro culture for plant improvement. IAEA, Vienna, pp 43–57

    Google Scholar 

  • Bajaj YPS (1986 b) Cryopreservation of potato somoclones. In: Semal J (ed) Somaclonal variations in crop improvement. Nijhoff, Dordrecht Lancaster, pp 244–250

    Google Scholar 

  • Bajaj YPS (ed) (1986c) Biotechnology in agriculture and forestry 2. Crops I. Springer, Berlin Heidelberg New York Tokyo

    Google Scholar 

  • Bajaj YPS (1987) Cryopreservation of pollen and pollen-embryos, and the establishment of pollenbanks. In: Giles KL, Prakash J (eds) Pollen development and cytology. Academic Press, London New York (in press)

    Google Scholar 

  • Bajaj YPS, Dionne LA (1967) Organ formation in potato callus by plating cell suspensions. Am J Bot 54:640

    Google Scholar 

  • Bajaj YPS, Dionne LA (1968) The continuous culture of excised potato roots. N Z J Bot 6:386–394

    Google Scholar 

  • Bajaj YPS, McAllan W (1969) Effect of various light treatments on chlorophyll formation in excised potato roots. Physiol Plant 22:25–28

    Article  Google Scholar 

  • Bajaj YPS, Reinert J (1977) Cryobiology of plant cell cultures and establishment of gene-banks In: Reinert J, Bajaj SYPS (eds) Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer, Berlin Heidelberg New York, pp 757–777

    Google Scholar 

  • Chen HH, Gavinlertavatana P, Li PH (1979) Cold acclimation of stemcultured plants and leaf callus of Solarium species. Bot Gaz 140:142–147

    Article  Google Scholar 

  • Grout BWW, Henshaw GG (1978) Freeze preservation of potato shoot tip cultures. Ann Bot (London) 42:1227–1229

    Google Scholar 

  • Hellergren J, Li PH (1981) Survival of Solarium tuberosum suspension cultures to -14 °C. The mode of action of proline. Physiol Plant 52:449–453

    Article  CAS  Google Scholar 

  • Howard HW (1958) The storage of potato pollen. Am Potato J 35:676

    Article  Google Scholar 

  • King JR (1955) Irish potato pollen storage. Am Potato J 32:460

    Article  Google Scholar 

  • Roest S, Bokelmann GS (1976) Vegetative propagation of Solarium tuberosum L. in vitro. Potato Res 19:173–178

    Article  CAS  Google Scholar 

  • Towill LE (1981a) Solarium etuberosum A model for studying the cryobiology of shoot-tips in the tuber bearing (Solarium) species. Plant Sci Lett 20:315–324

    Article  Google Scholar 

  • Towill LE (1981b) Liquid nitrogen preservation of pollen from tuber bearing Solarium species. Hort Sci 16:177–179

    Google Scholar 

  • Montezuma-de-Carvalho J, Guimaraes MLL (1976) Isolation of a callus from potato roots. Bol Soc Borteriana (2 a Ser) L:143–163

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Wheatherhead MA, Grout BWW, Henshaw GG (1978) Advantages of storage of potato pollen in liquid nitrogen. Potato REs 21:331–334

    Article  Google Scholar 

  • White PR (1934) Multiplication of viruses of tobacco and aucuba mosaic in growing excised tomato roots. Phytopathology 24:1003–1011

    Google Scholar 

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© 1987 Springer-Verlag Berlin Heidelberg

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Bajaj, Y.P.S. (1987). Cryopreservation of Potato Germplasm. In: Bajaj, Y.P.S. (eds) Potato. Biotechnology in Agriculture and Forestry, vol 3. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72773-3_33

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  • DOI: https://doi.org/10.1007/978-3-642-72773-3_33

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-72775-7

  • Online ISBN: 978-3-642-72773-3

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