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Symmetric Versus Asymmetric Fusion Combinations in Higher Plants

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Plant Protoplasts and Genetic Engineering I

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 8))

Abstract

The terms symmetric and asymmetric hybrids refer to the nuclear constitution of fusion products. Symmetric hybrids are defined as consisting of complete sets of chromosomes from both the parents, while asymmetric ones result from a preferential or unidirectional loss of chromosomes belonging to one of the fusion partners. Since protoplasts can be isolated from almost any plant species and readily induced to fuse in all possible combinations, it was expected and even accepted that fusion technology was going to make important contributions in both basic and applied research. This matter was critically evaluted by Harms (1983 a), Burgess (1984) and reviewed in detail by Gleba and Sytnik (1984) and Horn et al. (1986).

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References

  • Ambros PF, Matzke MA, Matzke AJM (1986) Detection of 17 kb unique sequence (T-DNA) in plant chromosomes by in situ hybridization. Chromosoma 94:11–18

    Article  CAS  Google Scholar 

  • Bayliss MW (1975) The effect of growth in vitro on the chromosome complement of Daucus carota suspension cultures. Chromosoma 51:401–411

    Article  Google Scholar 

  • Binding H (1976) Somatic hybridization experiments in Solanaceous species. Mol Gen Genet 144:171–175

    Article  Google Scholar 

  • Binding H, Nehls R (1978) Somatic cell hybridization of Vicia faba + Petunia hybrida. Mol Gen Genet 164:137–143

    Article  Google Scholar 

  • Brar DS, Khush GS (1986) Wide hybridization and chromosome manipulation in cereals. In: Evans DA, Sharp WR, Ammirato PV (eds) Handbook of plant cell culture, Vol. 4. Macmillan, New York, pp 221–263

    Google Scholar 

  • Brunold C, Kruger-Lebus C, Saul MW, Wegmuller S, Potrykus I (1987) Combination of kanamycin resistance and nitrate reductase deficiency as selectable markers in one nuclear genome provides a universal somatic hybridizer in plants. Mol Gen Genet 208:469–473

    Article  CAS  Google Scholar 

  • Burgess J (1984) The revolution that failed. New Sci 104/1428:26–29

    Google Scholar 

  • Constabel F, Weber G, Kirkpatrick JW, Pahl K (1976) Cell division of intergeneric protoplast fusion products. Z Pflanzenphysiol 79:1–7

    Google Scholar 

  • Dirks R, Negrutiu I, Heinderickx M, Jacobs M (1986) Genetic analysis of revertants for the nitrate reductase function in Nicotiana plumbaginifolia. Mol Gen Genet 202:309–311

    Article  CAS  Google Scholar 

  • Dudits D, Hadlaczky G, Bajszar GY, Kouczc C, Lazar G, Horvath G (1979) Plant regeneration from intergeneric cell hybrids. Plant Sci Lett 15:101–112

    Article  CAS  Google Scholar 

  • Dudits D, Fejer O, Hadlaczky F, Koucz C, Lapar G, Horvath G (1980) Intergeneric gene transfer mediated by plant protoplast fusion. Mol Gen Genet 179:283–288

    Article  Google Scholar 

  • Endo T, Komiya T, Masumitsu Y, Morikawa H, Yamada Y (1987) An intergeneric hybrid cell line of Duboisia hopwoodii and N. tabacum by protoplast fusion. J Plant Physiol 129:453–459

    Google Scholar 

  • Famelaer Y, Cammaerts D, Karp A, Sidorov V, De Brouwer D, Negrutiu I, Jacobs M (1986) Cellular engineering by “gamma fusion” and “egg transformation”. In: Nuclear techniques and in vitro culture for plant improvement. International Atomic Energy Agency Vienna, IAEA-SM-282/44, pp 453–461

    Google Scholar 

  • Fitter MS, Normann PM, Hahn MG, Wingate VPM, Lamb C (1987). Identification of somatic hybrids in plant protoplast fusion with monoclonal antibodies to plasma-membrane antigens. Planta 170:49–54

    Article  Google Scholar 

  • Flick CE, Kut SA, Bravo JE, Gleba Yu, Evans DA (1985) Segregation of organelle traits following protoplast fusion in Nicotiana. Biotechnology 3:555–560

    Article  CAS  Google Scholar 

  • Fluhr R, Aviv D, Edelman M, Grahon E (1983) Cybrids containing mixed and sorted-out chloroplasts following interspecific somatic fusions in Nicotiana. Theor Appl Genet 65:289–294

    Article  Google Scholar 

  • Gleba YuYu, Sytnik KM (1984) Protoplast fusion — Genetic engineering in higher plants. Monographs on theoretical and applied genetics, Vol 8. Springer, Berlin Heidelberg New York Tokyo

    Google Scholar 

  • Gleba YuYu, Momot VP, Cherep NN, Skarzhynskaya MV (1982). Intertribal hybrid cell lines of Atropa belladonna × Nicotiana chinensis obtained by cloning individual protoplast fusion products. Theor Appl Genet 62:75–79

    Google Scholar 

  • Gleba YuYu, Momot VP, Okolot AN, Cherep NN, Skarzhynskaya MV, Kotov V (1983) Genetic processes in intergeneric cell hybrids Atropa + Nicotiana. Theor Appl Genet 65:269–276

    Article  Google Scholar 

  • Glimelius K (1985) Sexual and somatic hybridization. Hereditas Suppl 3:41–47

    Google Scholar 

  • Glimelius K, Fahlesson J, Sjödin C, Sundberg E, Djupsjöbacka M, Fellner-Feldegg H, Bonnet HT (1986) Somatic hybridization and cybridization as potential methods for widening of the gene-pools of crops within Brassicaceae and Solanaceae. In: Horn W, Jensen CJ, Odenbach W, Schieder O (eds) Genetic Manipulation in plant breeding. Proceedings International Symposium Eucarpia, Sept. 8-13, 1985, Berlin, de Gruyter, Berlin, pp 663–682

    Google Scholar 

  • Gould AR, Daines RJ (1986) Plant protoplasts and the cell cycle. In: Fowke LC, Constabel F (eds) Plant Protoplasts. CRC Press Inc, Boca Raton Florida, pp 67–77

    Google Scholar 

  • Grimsley N, Hohn T, Davies W, Hohn B (1987) Agrobacterium-mediated delivery of infectious maize streak virus into maize. Nature 325:177–179

    Article  CAS  Google Scholar 

  • Gupta PP, Schieder O, Gupta M (1984) Intergeneric nuclear gene transfer between somatically and sexually incompatible plants through asymmetric protoplast fusion. Mol Gen Genet 197:30–35

    Article  CAS  Google Scholar 

  • Hamill JD, Pental D, Cocking EC (1985) Analysis of fertility in somatic hybrids of Nicotiana rustica and N. tabacum and progeny over two sexual generations. Theor Appl Genet 71:486–490

    Article  Google Scholar 

  • Harms CT (1983a) Somatic hybridization by plant protoplast fusion. In: Potrykus I, Harms CT, Hin-nen A, Hutter R, King PJ, Shillito RD (eds) Protoplasts 1983. Birkhäuser, Basel, pp 69–84

    Google Scholar 

  • Harms CT (1983 b). Somatic incompatibility in the development of higher plant somatic hybrids. Q Rev Biol 58:325–351

    Article  Google Scholar 

  • Hayashi J, Yonekawa H, Murakami J, Tagashira Y, Perreirra-Smith OM, Shay JW (1987) Mitochon-drial genomes in intraspecies mammalian cell hybrids display codominant or dominant/recessive behaviour. Exp Cell Res 172:218–227

    Article  PubMed  CAS  Google Scholar 

  • Hoffmann F, Adachi T (1981) Arabidobrassica: chromosomal recombination and morphogenesis in asymmetric intergeneric hybrid cells. Planta 153:586–593

    Article  Google Scholar 

  • Horn W, Jensen CJ, Odenbach W, Schieder O (eds) (1986) Genetic manipulation in plant breeding. Proceedings International Symposium Eucarpia, Sept. 8-13, 1985, Berlin, de Gruyter, Berlin

    Google Scholar 

  • Imamura J, Saul MW, Potrykus J (1987) X-ray irradiation promoted asymmetric somatic hybridization and molecular analysis of the products. Theor Appl Genet 74:445–450

    Article  CAS  Google Scholar 

  • Installe P, Negrutiu I, Jacobs M (1985) Protoplast-derived plants in Nicotiana plumbaginifolia: improving the regeneration response of wild type and mutant cultures. J Plant Physiol 119:443–454

    CAS  Google Scholar 

  • Jia J, Potrykus I, Lazar GB, Saul M (1983) Hyoscyamus-Nicotiana fusion hybrids selected via auxotroph complementation and verified by species-specific DNA hybridization. In: Potrykus I, Harms CT, Hinnen A, Hutter R, King PJ, Shillito RD (eds) Protoplasts 1983. Birkhäuser, Basel, pp 110–111

    Google Scholar 

  • Kushnir SG, Schlumukov LR, Pogrebnyak NJ, Berger S, Gelba Yu (1987) Functional cybrid plants possessing a Nicotiana genome and an Atropa plastome. Mol Gen Genet 209:159–163

    Article  PubMed  CAS  Google Scholar 

  • Lima-de-Faria A, Hiraoka T, Jarl C, Bornman CH, Joworska H (1983) Fusion of bull and human with Nicotiana protoplasts. Hereditas 99:191–201

    Article  Google Scholar 

  • Lo Schiavo F, Giovinazzo G, Terzi M (1983) 8-Azaguanine resistant carrot cell mutants and their use as universal hybridizers. Mol Gen Genet 192:326–329

    Article  Google Scholar 

  • Maliga P, Lazar G, JoO’ I, Nagy AH, Menczel L (1977) Restoration of morphogenic potential in Nicotiana by somatic hybridization. Mol Gen Genet 157:291–296

    Article  CAS  Google Scholar 

  • Medgyesy P, Feyes E, Maliga P (1985) Interspecific chloroplast recombination in a Nicotiana somatic hybrid. PNAS 82:6960–6964

    Article  PubMed  CAS  Google Scholar 

  • Medgyesy P, Pay A, Marton L (1986) Transmission of parental chloroplasts in Nicotiana. Mol Gen Genet 204:195–198

    Article  CAS  Google Scholar 

  • Melchers G (1977) Plant hybrids by fusion of protoplasts. In: Beers RF, Bassett EG (eds) Recombinant molecules: impact on science and society. Raven, New York, pp 209–227

    Google Scholar 

  • Menczel L, Galiba G, Nagy F, Maliga P (1982) Effect of radiation dosage on efficiency of chloroplast transfer by protoplast fusion in Nicotiana. Genetics 100:487–495

    PubMed  CAS  Google Scholar 

  • Morgan A, Maliga P (1987) Rapid chloroplast segregation and recombination of mitochondrial DNA in Brassica cybrids. Mol Gen Genet 209:240–246

    Article  PubMed  CAS  Google Scholar 

  • Mouras A, Salessess G, Lutz A (1978) Sur l’utilisation de protoplastes en cytologie: amélioration D’une méthode récente en vue de l’identification des chromosomes mitotiques des genres Nicotiana et Prunus. Caryologia 31:117–127

    Google Scholar 

  • Mouras A, Wildenstein C, Salesses G (1986) Analysis of karyotype and C-banding pattern of N. plumbaginifolia using two techniques. Genetica 68:197–203

    Article  Google Scholar 

  • Mouras A, Saul MW, Essad S, Potrykus I (1987) Localization by in situ hybridization of a low copy chimaeric resistance gene introduced into plants by direct gene transfer. Mol Gen Genet 207:204–209

    Article  CAS  Google Scholar 

  • Negrutiu I, Jacobs M, Cattoir-Reynaerts A (1984) Progress in cellular engineering of plants: biochemical and genetic assessment of selectable markers from cultured cells. Plant Mol Biol 3:289–302

    Article  CAS  Google Scholar 

  • Negrutiu I, De Brouwer D, Watts JW, Sidorov VI, Dirks R, Jacobs M (1986) Fusion of plant protoplasts: a study using auxotrophic mutants of Nicotiana plumbaginifolia. Theor Appl Genet 72:279–286

    Article  Google Scholar 

  • Negrutiu I, Mouras A, Horth M, Jacobs M (1987) Direct gene transfer to plants: present developments and some future prospectives. Plant Physiol Biochem 25:493–503

    CAS  Google Scholar 

  • Nehls R (1978) The use of metabolic inhibitors for the selection of fusion products of higher plant protoplasts. Mol Gen Genet 166:117–118

    Article  Google Scholar 

  • Nicoll MF, Chapman GP, James DJ (1987) Endosperm responses to irradiated pollen in apples. Theor Appl Genet 74:508–515

    Article  Google Scholar 

  • O’Connell MA, Hanson MR (1985) Regeneration of somatic hybrid plants formed between Lycoper-sicon aesculentum and Solanum rickii Theor Appl Genet 72:59–65

    Google Scholar 

  • Pelletier G, Vedel F, Belliard G (1985) Cybrids in genetics and breeding. Hereditas Suppl 3:49–56

    Google Scholar 

  • Pental D, Hamill DJ, Pirrie A, Cocking EC (1986) Somatic hybridization of Nicotiana tabacum and Petunia hybrida. Recovery of plants with P. hybrida nuclear genome and Nicotiana tabacum chloroplast genome. Mol Gen Genet 202:342–347

    Article  CAS  Google Scholar 

  • Poulsen C, Porath D, Sacristan MD, Melchers G (1980) Peptide mapping of the ribulese biphosphate carboxylase small subunit from the somatic hybrid of tomato and potato. Carlsberg Res Commun 45:249–267

    Article  CAS  Google Scholar 

  • Puite KJ, Van Wikselaar P, Verhoeven H (1985) Electrofusion, a simple and reproducible technique in somatic hybridization of Nicotiana plumbaginifolia mutants. Plant Cell Rep 4:274–276

    Article  Google Scholar 

  • Rothenberg M, Hanson MR (1987) Recombination between parental mitochondrial DNA following protoplast fusion can occur in a region which normally does not undergo intragenomic recombination in parental plants. Curr Genet 12:235–240

    Article  CAS  Google Scholar 

  • Saul MW, Potrykus I (1984) Species-specific repetitive DNA used to identify interspecific somatic hybrids. Plant Cell Rep 3:65–67

    Article  CAS  Google Scholar 

  • Schieder O, Gupta PP, Krumbiegel-Schroener G, Hein T, Steffen A (1985) Novel techniques in handling and manipulating cells. Hereditas Suppl 3:65–75

    Google Scholar 

  • Schweiger HG, Dirk J, Koop HU, Kranz E, Neuhaus G, Spangenberg G, Wolff D (1987) Individual selection, culture, and manipulation of higher plant cells. Theor Appl Genet 73:769–783

    Article  Google Scholar 

  • Sidorov VA, Zubco MK, Kuchko AA, Komanitsky IK, Gleba YuYu (1987) Somatic hybridization in potato: use of gamma-irradiated protoplasts of Solanum pinnatisectum in genetic reconstruction. Theor Appl Genet 74:346–368

    Article  Google Scholar 

  • Singh G, Neckelmann N, Wallace DC (1987) Conformational mutations in human mitochondrial DNA. Nature 329:270–272

    Article  PubMed  CAS  Google Scholar 

  • Somers DA, Narayanan KR, Kleinhofs A, Cooper-Blaud S, Cocking EC (1986) Immunological evidence for transfer of the barley nitrate reductase structural gene to Nicotiana tabacum by protoplast fusion. Mol Gen Genet 204:296–301

    Article  CAS  Google Scholar 

  • Spangenberg G, Schweiger HG (1986) Controlled electrofusion of different types of protoplasts including cell reconstitution in Brassica napus. Eur J Cell Biol 41:51–56

    Google Scholar 

  • Spangenberg G, Neuhaus G, Schweiger HG (1986) Expression of foreign genes in a higher plant cell after electrofusion-mediated cell reconstitution of a microinjected karyoplast and a cytoplast. Eur J Cell Biol 42:236–238

    CAS  Google Scholar 

  • Tempelaar MJ, Jones MGK (1985) Directed electrofusion between protoplasts with different responses in a mass function system. Plant Cell Rep 4:92–95

    Article  Google Scholar 

  • Ward M (1984) Fusion of plant protoplasts with animal cells. In: Beers RF, Bassett EG (eds) Cell fusion: gene transfer and transformation. Raven, New York, pp 189–197

    Google Scholar 

  • Watts JW, King JM (1984) A simple method for large-scale electrofusion and culture of plant protoplasts. Biosci Rep 4:335–342

    Article  PubMed  CAS  Google Scholar 

  • Ye J, Hauptmann RM, Smith AG, Widholm JM (1987) Selection of a Nicotiana plumbaginifolia universal hybridizer and its use in intergeneric somatic hybrid formation. Mol Gen Genet 208:474–480

    Article  CAS  Google Scholar 

  • Zuckerman SH, Gillespie FP, Solus JF, Rybczynski R, Eisenstadt JM (1986) Mitochondrial protein synthesis in interspecific somatic cell hybrids. Somatic Cell Mol Genet 12:449–458

    Article  CAS  Google Scholar 

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© 1989 Springer-Verlag Berlin Heidelberg

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Negrutiu, I. et al. (1989). Symmetric Versus Asymmetric Fusion Combinations in Higher Plants. In: Bajaj, Y.P.S. (eds) Plant Protoplasts and Genetic Engineering I. Biotechnology in Agriculture and Forestry, vol 8. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73614-8_21

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  • DOI: https://doi.org/10.1007/978-3-642-73614-8_21

  • Publisher Name: Springer, Berlin, Heidelberg

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