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Transport in Plasma Membrane Vesicles — Approaches and Perspectives

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The Plant Plasma Membrane

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Abstract

Preparations of isolated vesicles represent an ideal system for the study of transport processes associated with native membranes. With this approach, it is possible to characterize single transport systems without the complications associated with examining transport in the intact cell. Since the vesicles are not associated with the metabolic machinery of the cell, solute transport can be studied in the absence of metabolic transformation or compartmentation and the energy supply to drive transport (e.g., ATP) can be added exogenously.

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Abbreviations

BTP:

bis-tris propane

CCCP:

carbonylcyanide m-chlorophenylhydrazone

DES:

diethylstilbestrol

DCCD:

N,N′-dicyclohexylcarbodiimide

EPR:

electron paramagnetic resonance

PMSF:

phenylmethylsulfonyl fluoride

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Authors and Affiliations

  1. Department of Agronomy, University of Illinois, 1102 S. Goodwin Avenue, Urbana, IL, 61801, USA

    D. P. Briskin

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  1. D. P. Briskin
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Editors and Affiliations

  1. Department of Plant Biochemistry, University of Lund, Box 7007, S-220 07, Lund, Sweden

    Christer Larsson

  2. Department of Plant Physiology, University of Lund, Box 7007, S-220 07, Lund, Sweden

    Ian M. Møller

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© 1989 Kluwer Academic Publishers

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Briskin, D.P. (1989). Transport in Plasma Membrane Vesicles — Approaches and Perspectives. In: Larsson, C., Møller, I.M. (eds) The Plant Plasma Membrane. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74522-5_7

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  • DOI: https://doi.org/10.1007/978-3-642-74522-5_7

  • Publisher Name: Springer, Berlin, Heidelberg

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