Abstract
The recent demonstration that C.B-17scid/scid mice (hereafter referred to as SCID mice) could be grafted successfully with human haematopoietic cells (Hosier et al., 1988; McCune et al., 1988; Kamel-Reid and Dick, 1988) has opened the way to the use of the SCID-hu mouse as a model for studying human immune-function in vivo. However, if SCID-hu mice are generated by intraperitoneal injection of about 5×107 human peripheral blood lymphocytes (PBLs), then consideration must be given to the fact that human B cell lymphomas regularly develop between 2–4 months post-engraftment. The causal role of Epstein-Barr virus (EBV) in the development of these lymphomas has been demonstrated (Mosier et al., 1988). Thus, all of the lymphomas contained EBV DNA and, furthermore, lymphomas did not develop in mice engrafted with PBLs from donors where there was no serological evidence of EBV infection. In this preliminary report we have further characterised these EBV-positive lymphomas in order to determine whether they might be used as a model for EBV-associated malignancies.
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References
Cleary ML, Dorfman RF, Sklar J (1986) Failure in immunological control of the virus infection: Post-transplant lymphomas. In: Epstein MA, Achong BG (eds) The Epstein-Barr Virus: Recent Advances. William Heinemann Medical Books, London, pp 163–182
Gregory CD, Rowe M, Rickinson AB (1990) Different Epstein-Barr virus (EBV)-B cell interactions in phenotypieally distinct clones of a Burkitt lymphoma cell line. J. Gen. Virol. (in press)
Henle W, Henle G (1985) Epstein-Barr virus and human malignancies. In: Klein G (ed) Adv. Viral Oncology vol. 5. Raven Press, New York. pp 201–238
Kamel-Reid S, Dick JE (1988) Engraftment of immune-deficient mice with human hematopoietic stem cells. Science 242: 1706–1709
Knutson JC, Sugden B (1989) Immortalization of B lymphocytes by Epstein-Barr virus: What does the virus contribute to the cell? In: Klein G (ed) Adv. Viral Oncology vol. 8. Raven Press, New York. pp 151–172
Lenoir GM, Bornkamm (1987) Burkitt’s lymphoma, a human cancer model for the study of the multistep development of cancer: proposal for a new scenario. In: Klein G (ed) Adv. Viral Oncology vol. 7. Raven Press, New York. pp 173–206
McCune JM, Namikawa R, Kaneshima H, Schultz LD, Lieberman M, Weissman IL (1988) The SCID-hu mouse: Murine model for the analysis of human hematolymphoid differentiation and function. Science 241: 1632–1639
Mosier DE, Gulizia RJ, Baird SM, Wison DB, (1988) Transfer of a functional human immune system to mice with severe combined immunodeficiency. Nature 335: 256–259
Rickinson AB (1986) Cellular immunological responses to the virus infection. In: Epstein MA, Achong BG (eds) The Epstein-Barr Virus: Recent Advances. William Heinemann Medical Books, London. pp 75–126
Rowe M, Rowe DT, Gregory CD, Young LS, Farrell PJ, Rupani H, Rickinson AB (1987) Differences in B cell growth phenotype reflect novel patterns of Epstein-Barr virus latent gene expression in Burkitt’s lymphoma. EMBO J. 6: 2743–2751
Rowe M, Gregory C (1989) Epstein-Barr virus and Burkitt’s lymphoma. In: Klein G (ed) Adv. Viral Oncology vol. 8. Raven Press, New York. pp 237–259
Shapiro RS, McClain K, Frizzera G, Gajl-Peczalska KJ, Kersey JH, Blazer BR, Arthur DC, Patton DF, Greenberg JS, Burke B, Ramsay NKC, McGlave P, Filipovich AH (1988) Epstein-Barr virus associated B cell lymphoproliferative disorders following bone marrow transplantation. Blood 71:1234–1243
Torsteinsdottir S, Brautbar C, Klein G, Klein E, Masucci MG (1988) Differential expression of HLA antigens on B-cell lines of normal and malignant origin: a consequence of immune surveillance or a phenotypic vestige of the progenitor cells? Int. J. Cancer 41: 913–919
Young L, Alfieri C, Hennessey K, Evans H, O’Hara C, Anderson KC, Ritz J, Shapiro RS, Rickinson A, Kieff E, Cohen JI (1989) Expression of Epstein-Barr virus transformation-associated genes in tissues of patients with EBV lympho proliferative disease. N. Eng. J. Med. 321: 1080–1085
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Rowe, M., Young, L.S., Rickinson, A.B. (1990). Analysis of Epstein-Barr Virus Gene Expression in Lymphomas Derived from Normal Human B Cells Grafted into SCID Mice. In: Potter, M., Melchers, F. (eds) Mechanisms in B-Cell Neoplasia 1990. Current Topics in Microbiology and Immunology, vol 166. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75889-8_40
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DOI: https://doi.org/10.1007/978-3-642-75889-8_40
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