Abstract
In 1986 Langenberg and colleagues [5] showed that chromosomal DNA restriction endonuclease digest analysis provided a sensitive between isolates of Helicobacter pylori. Since then such data heve been used increasingly to characterize clinical isolates of H. pylori in treatment and epidemiological studies (for reviews see [3,9]). The total DNA patterns are generally complex and difficult to apply to large numbers of strains, but in 1990 Morgan and Owen [6] demonstrated that simpler fingerprints highlighting specific DNA sequences, namely ribosomal (r)RNA genes, within total digests. Subsequent studies provided further evidence that rRNA gene probe patterns (ribopatterns) are a simple but highly discriminatory electrophoretic fingerprint for H. pylori [9–12, 16]. The aim of the present report is to describe some recent developments and applications of ribopatterns to typing H. pylori.
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References
Bukholm G, Nedenskov-Sorensen P, Bovre K (1989) DNA-DNA hybridization incompatibility of Campylobacter pylori with other Campylobacter and Wolinella species. APMIS 97: 472–474
Costas M (1990) Numerical analysis of sodium dodecyl sulphate — polyacrylamide gel electrophoretic protein patterns for the classification, identification and typing of medically important bacteria. Electrophoresis 11:382–391
Costas M, Owen RJ, Bickley J, Morgan DR (1991) Molecular techniques for studying the epidemiology of infection by Helicobacter pylori. Scand J Gas roentero l26 [Suppl181]: 20–32
Grimont F, Grimont PAD (1986) Ribosomal ribonucleic acid gene restrietion patterns as potential taxonomie tools. Ann Inst Pasteur Microbiol 137B: 165–175
Langenberg W, Rauws EAJ, Widjojokusomo A et al. (1986) Identification of Campylobacter pylori isolates by restriction endonuclease analysis. J Clin Microbiol 24: 414–417
Morgan DD, Owen RJ (1990) Use of DNA restriction digest and ribosomal RNA gene probe patterns to fingerprint Helicobacter pylori and Helicobacter mustelae isolated from human and animal hosts. Molec Cell Prob 4:321–334
Owen RJ (1989) Chromosomal DNA fingerprinting - a novel method of species and strain identification applicable to microbial pathogens. J Med Microbiol 30:89–99
Owen RJ, Beck A (1987) Evaluation of three procedures using a laser densitometer and microcomputer for estimating molecular sizes of restriction endonuclease digest fragments and application toCampylobacter jejuni chromosomal DNA. Lett Appl Microbiol 4:5–8
Owen RJ, Bickley J, Costas M, Morgan DR (1991) Genomic variation of Helicobacter pylori: application to identification of strains. Scand J Gastroenterol 26 [Suppl 181]: 43–50
Owen RJ, Bickley J, Moreno M et al. (1991) Biotype and macromolecular profiles of cytotoxin- producing strains of Helicobacter pylori from antral gastric mucosa. FEMS Microbiol Lett 79:199–204
Owen RJ, Bickley J, Lastovica A et al. (1992) Ribosomal RNA gene patterns ofHelicobacter pylori from surgical patients with healed and recurrent peptic ulcers. Epidemiol Infect 108:39–50
Owen RJ, Figura N, Moreno M (1992) Biotype and DNA fingerprints of cytotoxigenic Helicobacter pylori from patients with gastritis and peptic ulceration in Italy. Eur J Epidemiol 8:15–21
Pitcher DG, Saunders NA, Owen RJ (1989) Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8:151–156
Paster BJ, Dewhirst FE (1988) Phylogeny of Campylobacters, wolinellas, Bacteroides gingivalis, and Bacteroides ureolyticus by 16S ribosomal ribonucleic acid sequencing. Int J Syst Bacteriol 38:56–62
Pitcher DG, Owen RJ, Dyal P, Beck A (1987) Synthesis of a biotinylated DNA probe to detect ribosomal RNA cistrons in Providencia stuartii. FEMS Microbiol Lett 48:283–287
Prewett EJ, Bickley J, Owen RJ, Pounder RE (1992) DNA patterns of Helicobacter pylori isolated from gastric antrum, body, and duodenum. Gastroenterology 102:829–833
Sneath PHA, Sokal RR (1973) Numerical taxonomy. Freeman, San Francisco
Stull TL, LiPuma JJ, Edlind TD (1988) A broad spectrum probe for molecular epidemiology of bacteria: ribosomal RNA. J Infect Dis 157:280–286
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© 1994 Springer-Verlag Berlin Heidelberg
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Owen, R.J. (1994). Developments in Ribotyping of Helicobacter pylori . In: Gasbarrini, G., Pretolani, S. (eds) Basic and Clinical Aspects of Helicobacter pylori Infection. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78231-2_40
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DOI: https://doi.org/10.1007/978-3-642-78231-2_40
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