Abstract
Autoantibodies against double-stranded DNA (dsDNA) are a characteristic marker of human systemic lupus erythematosus (SLE) and its murine models. These antibodies play a major role in the pathogenic mechanisms, since their titers correlate with disease activity and deposits of DNA/anti-DNA immune complexes are found in diseased organs (Tan, 1989). Furthermore, nephritis has been induced in SLE-prone mice by injection of monoclonal anti-dsDNA antibodies (Vlahakos et al, 1992). The typical SLE anti-dsDNA antibody is of high-affinity and of the IgG isotype. This contrasts with the IgM anti-DNA antibodies of the so-called “natural autoantibody repertoire” which can be isolated not only from SLE patients but also from normal donors. These antibodies are of lower affinity, polyreactive and prefer single-stranded DNA (ssDNA) to dsDNA as the target antigen. We have generated human monoclonal anti-dsDNA antibodies of the IgG isotype by direct fusion of peripheral blood lymphocytes isolated from patients with SLE with the cell line CB-F7. The the variable region genes of the antibodies were sequenced and compared to the most homologous germline genes.
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© 1994 Springer-Verlag Berlin Heidelberg
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Fehr, H.K., Winkler, T.H., Kalden, J.R. (1994). Molecular analysis of anti-dsDNA autoantibodies from patients with systemic lupus erythematosus. In: Zouali, M. (eds) Autoimmunity: Experimental Aspects. NATO ASI Series, vol 80. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78779-9_12
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DOI: https://doi.org/10.1007/978-3-642-78779-9_12
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