Summary
In this minireview we summarize our studies on the consequences of acylation for the structure and function of the transmembrane helical peptide gramicidin A. It is shown that acylation of the C-terminal ethanolamine group does not largely affect the polypeptide structure and conformation. However, the lifetime of the cation specific channels formed in black lipid membranes is greatly increased upon acylation. The conformation of the covalently attached acyl chain in bilayers of dimyristoylphosphatidylcholine was analyzed by 2H NMR in oriented systems using palmitoylgramicidin in which the acyl chain was either per- or specifically deuterated. From C8 towards the methyl end the order and dynamics of the covalently attached acyl chains follow closely that of the phospholipids in a liquid crystalline bilayer. In contrast, the carboxyl part of the acyl chain is highly ordered and takes up a defined conformation. The results are discussed in the light of the structural and functional consequences of acylation of transmembrane proteins.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Beers MF and Fisher AB (1992) Surfactant protein C–A review of its unique properties and metabolism. Am J Physiol 63: 1151–1160
Killian JA (1992) Gramicidin and gramicidin-lipid interactions. Biochim Biophys Acta 1113: 391–425
Schmidt MFG (1989) Fatty acylation of proteins. Biochim Biophys Acta 988: 411–426
Schultz AM, Henderson LE and Oroszlan S. (1988) Fatty acylation of proteins. Annu Rev Cell Biol 4: 611–647
Seelig J and Niederberger W (1974) Deuterium labeled lipids as structural probes in liquid crystalline bilayers. J Am Chem Soc 96: 2069–2072
Taylor MJ, Mattice GL, Hinton JF and Koeppe RE II (1991) NMR
studies of acylated gramicidin in d6-DMSO solution and d25-SDS micelles. Biophys J 59:319a
Vogt TCB, Killian JA, Demel RA and De Kruiff B (1991) Synthesis of acylated gramicidins and the influence of acyation on the interfacial properties and conformational behavior of gramicidin A. Biochim Biophys Acta 1069: 157–164
Vogt TCB, Killian JA, De Kruijff B and Andersen OS (1992) Influence of acylation on the channel characterstics of gramicidin A. Biochemistry 31: 7320–7324
Vogt TCB, Killian JA and De Kruijff B (1993) The structure and dynamics of the acyl chain of a palmitoylated transmembrane polypeptide. Biochemistry, submitted
Williams LP, Narcessian EJ, Andersen OS, Waller GR, Taylor MJ, Lazenby JP, Hinton JF and Koeppe RE II (1992) Molecular and channel forming characteristics of gramicidin Ks: a family of naturally occuring gramicidins. Biochemistry 31: 7311–7319
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1994 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Vogt, T.C.B., Killian, J.A., de Kruijff, B. (1994). Structural and Functional Consequences of Acylation of a Transmembrane Peptide. In: Op den Kamp, J.A.F. (eds) Biological Membranes: Structure, Biogenesis and Dynamics. NATO ASI Series, vol 82. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78846-8_12
Download citation
DOI: https://doi.org/10.1007/978-3-642-78846-8_12
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-78848-2
Online ISBN: 978-3-642-78846-8
eBook Packages: Springer Book Archive