Abstract
Sodium dodecylsulphate (SDS) consists of an aliphatic chain of 12 C atoms to which at one end a sulphate residue is bound. It forms complexes with both the polar and non- polar amino acid residues of proteins irrespective of their sizes and shapes, leaving the primary structure uninfluenced. In electrophoresis SDS is used:
-
a)
to separate (enzyme) proteins into their monomeric constituents,
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b)
to estimate the molecular mass of unfolded (and reduced) polypeptides, and
-
c)
to keep membrane proteins in a solubilized state.
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© 1994 Springer-Verlag Berlin Heidelberg
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Rothe, G.M. (1994). Sodium Dodecylsulphate Electrophoresis. In: Electrophoresis of Enzymes. Springer Labmanual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79069-0_4
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DOI: https://doi.org/10.1007/978-3-642-79069-0_4
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