Abstract
One of the most classical and well known in vitro system is the Ames test, making use of a liver homogenate providing the metabolic competence needed for the detection of mutagenic reactive metabolites in Salmonella bacterium. Liver cells were isolated and have been tried over many years to maintain their differentiation status. The application of established cell lines for metabolism related studies was developed. The importance of cytochromes P450 became evident in the meantime, resulting into studies based on individual purified and reconstituted cytochromes P450. Finally, genetically engineered cell systems for metabolism studies were created (Doehmer 1993).
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References
Doehmer J (1993) V79 Chinese hamster cells genetically engineered for cytochrome P450 and their use in mutagenicity and metabolism studies. Toxicol 82:105–118
Jensen KG, Loft S, Doehmer J, Poulsen HE (1993) Metabolism of phenacetin in V79 Chinese hamster cell cultures expressing rat liver cytochrome P450 1A2 compared to isolated rat hepatocytes. Biochem Pharmacol 45:1171–1173
Moll R, Franke WW, Schiller DL (1982) The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells. Cell 31:11–24
Pfeifer AMA, Cole KE, Smoot DT, Weston A, Groopman JD, Shields PG, Vignaud JM, Juillerat M, Lipsky MM, Trump BF, Lechner JF, Harris CC (1993) Simian virus 40 large tumor antigenimmortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens. Proc Natl Acad Sci USA 90:5123–5127
Rogiers V, Vandenberghe Y, Callaerts A, Verleye G, Cornet M, Mertens K, Sonck W, Vercruysse A (1990) Phase I and phase II xenobiotic biotransformation in cultures and co-cultures of addult rat hepatocytes. Biochem Pharmacol 40:1701–1708
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© 1995 Springer-Verlag Berlin Heidelberg
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Doehmer, J. (1995). In Vitro Systems as Analytical Tools for Understanding and Predicting Drug Metabolism In Vivo. In: Arinç, E., Schenkman, J.B., Hodgson, E. (eds) Molecular Aspects of Oxidative Drug Metabolizing Enzymes. NATO ASI Series, vol 90. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79528-2_20
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DOI: https://doi.org/10.1007/978-3-642-79528-2_20
Publisher Name: Springer, Berlin, Heidelberg
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