Abstract
One of the most critical stages in producing a DNA fingerprint or DNA profile is hybridisation of the membrane-bound DNA samples to a labelled multilocus or single locus (VNTR) probe. Until the early 1990’s the probes used were usually prepared by radioactive labelling of purified plasmid inserts. More recently non-isotopic labelling systems have become available and probes for DNA fingerprinting and DNA profiling can now be purchased labelled and ready to use eg, NICE™ probes (Cellmark). Apart from their obvious safety and environmental advantages, non-isotopic probes allow reduced labour costs and more reproducible, high quality results and have therefore been very widely adopted (Ref. 1).
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A F Giles, K J Booth, J R Parker, A J Garman, D T Carrick, H Akhavan, A P Schaap. Rapid, simple Non-Isotopic Probing of Southern Blots for DNA Fingerprinting. In Advances in Forensic Haemogenetics 3. Eds H F Polesky and W R Mayr. Springer-Verlag Berlin Heidelberg 1990.
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© 1996 Springer-Verlag Berlin Heidelberg
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Childs, W.P., Rysiecki, G. (1996). Evaluation of Hybridisation Equipment for Use with Non Isotopically Labelled Probes. In: Carracedo, A., Brinkmann, B., Bär, W. (eds) 16th Congress of the International Society for Forensic Haemogenetics (Internationale Gesellschaft für forensische Hämogenetik e.V.), Santiago de Compostela, 12–16 September 1995. Advances in Forensic Haemogenetics, vol 6. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80029-0_105
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DOI: https://doi.org/10.1007/978-3-642-80029-0_105
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