Abstract
In photosynthetic reaction centers determination of ultrafast electron transfer rates as a function of the thermodynamical driving force ΔG allows conclusions on the mechanism of primary charge separation. In this paper we present femtosecond timeresolved fluorescence measurements on the primary donor species P* in reaction centers from Rhodobacter Sphaeroides R26 after modifying the energetics of the neighboring pigment, a bacteriochlorophyll molecule. The modification was achieved by thermally replacing the pigment by a specially designed derivative, the Ni-bacteriochlorophyll. Room temperature rates for the primary charge separation in the modified reaction centers are the same as in the native ones. This supports a sequential two step mechanism also in native reaction centers.
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Häberle, T., Lossau, H., Hartwich, G., Scheer, H., Michel-Beyerle, M.E. (1996). Ultrafast Electron Transfer in Modified Photosynthetic Reaction Centers from Rhodobacter Sphaeroides Measured by Fluorescence Upconversion. In: Waidelich, W., Hügel, H., Opower, H., Tiziani, H., Wallenstein, R., Zinth, W. (eds) Laser in Forschung und Technik / Laser in Research and Engineering. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80263-8_41
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DOI: https://doi.org/10.1007/978-3-642-80263-8_41
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