Abstract
The brain, one of the last frontiers for humankind, performs highly sophisticated information processing such as memory, recognition and thinking. Recently, functional imaging techniques, such as positron emission tomography (PET) and functional magnetic resonance imaging (MRI), have been revealing many new insights into such brain activities. However, further improvements in spatial and temporal resolution for these techniques are still needed to study detailed activities of neurons and their networks. To fill this gap, the so-called optical method has been developed by the pioneering groups (see reviews Cohen and Lesher 1986; Grinvald and Frostig 1988), and this method may be considered to be at an experimental stage, mainly because, in spite of special instrumentation and expertise, the data obtained are not easy to interpret as the network activities in the brain. In this sense, even now to many neuroscientists electrophysiology would still be the method of choice for such studies.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Antic S, Zecevic D (1995) Optical signals from neurons with internally applied voltage-sensitive dyes. J Neurosci 15:1392–1405
Chien CB, Pine J (1991) Voltage-sensitive dye recording of action potentials and synaptic potentials from sympathetic microcultures. Biophys J 60:697–711
Cohen LB, Lesher S (1986) Optical monitoring of membrane potential:methods of multisite optical measurement. Soc Gen Physiol Ser (USA) 40:71–99
Elias SA, Yae H, Ebner TJ (1993) Optical imaging of parallel fiber activation in the rat cerebellar cortex:spatial effects of excitatory amino acids. Neuroscience 52:771–786
Fukunishi K, Murai N, Uno H (1993) Spatiotemporal observation of guinea pig auditory cortex with optical recording. Jpn J Physiol (Suppl 1) 43:861–66
Grinvald A, Frostig RD, Lieke E, Hildesheim R (1988) Optical imaging of neuronal activity. Physiol Rev 68:1285–1366
Kinoshita K Jr, Ashikawa I, Saita N, Yoshimura H, Itoh H, Nagayama K, Ikegami A (1988) Electroporation of cell membrane visualized under a pulsed-laser fluorescence microscope. Biophys J 53:1015–1019
Lev-Ram V, Grinvald A (1987) Activity-dependent calcium transients in central nervous system myelinated axons revealed by the calcium indicator Fura-2. Biophys J 52:571–576
Momose-Sato Y, Sakai T, Komuro H, Hirota A, Kamino K (1991) Optical mapping of the early development of the response pattern to vagal stimulation in embryonic chick brain stem. J Physiol (Lond) 442:649–668
Orbach HS, Van Essen DC (1993) In vivo tracing of pathways and spatio-temporal activity patterns in rat visual cortex using voltage sensitive dyes. Exp Brain Res 94:371–392
Sawaguchi T (1994) Modular activation and suppression of neocortical activity in the monkey revealed by optical imaging. Neuroreport 6:185–189
Sinha SR, Patel SS, Saggau P (1995) Simultaneous optical recording of evoked and spontaneous transients of membrane potential and intracellular calcium concentration with high spatio-temporal resolution. J Neurosci Methods 60:49–60
Sugitani M, Sugai T, Tanifuji M, Onoda N (1994) Signal propagation from piriform cortex to the endopiriform nucleus in vitro revealed by optical imaging. Neurosci Lett 171:175–178
Sutor B, Hablitz JJ, Rucker F, ten Bruggencate G (1994) Spread of epileptiform activity in the immature rat neocortex studied with voltage-sensitive dyes and laser scanning microscopy. J Neurophysiol (USA) 72:1756–1768
Takahashi M, Tsuchiya H, Hosoi S, Hayakawa T (1993) Development of 50 x 50 fiber-array photodiode camera. Proc SPIE (USA) 1757:111–114
Wellis DP, Kauer JS (1994) GABAergic and glutamatergic syanaptic input to identified granule cells in salamander olfactory bulb. J Physiol (Lond) 475:419–430
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Hosoi, S., Tsuchiya, H., Takahashi, M., Kashiwasake-Jibu, M., Sakatani, K., Hayakawa, T. (1998). Time-Resolved Imaging of Membrane Potentials and Cytoplasmic Ions at the Cellular Level with a 50x50 Fiber Array Photodiode Camera. In: Isenberg, G. (eds) Modern Optics, Electronics and High Precision Techniques in Cell Biology. Principles and Practice. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80370-3_4
Download citation
DOI: https://doi.org/10.1007/978-3-642-80370-3_4
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-80372-7
Online ISBN: 978-3-642-80370-3
eBook Packages: Springer Book Archive