Abstract
The first clinical trial for gene transfer in humans was approved in the United States in 1989. In this trial, genetically marked lymphocytes that had been removed from a melanoma biopsy were transduced with a retroviral vector containing the gene conferring resistance to the neomycin analogue G-418, propagated ex vivo and returned to the patient. The purpose of this gene marking study was to determine if the G-418 resistant lymphocytes honed specifically to melanoma and to determine the lifespan of the marked lymphocytes. Results of various gene marker trials have been published (Dunbar et al. 1994; Brenner 1996). Marker trials have demonstrated some homing to tumors, and marked lymphocytes have been detectable for years past re-infusion.
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© 1998 Springer-Verlag Berlin Heidelberg
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McGarrity, G.J. (1998). Human Gene Therapy: Review and Outlook. In: Clynes, M. (eds) Animal Cell Culture Techniques. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80412-0_33
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DOI: https://doi.org/10.1007/978-3-642-80412-0_33
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