Abstract
Fluorescence spectroscopy of nucleic acids at room temperature has been limited by the short lifetimes of the excited states of purines and pyrimidines (1) and scarce information is available from measurements of the stationary emission. Recently attempts have been made by using the time structure of synchrotron radiation in order to obtain time resolved emission spectra (2,3). The large pulse width (1.7 ns) of the synchrotron pulse used has however prevented the measurement of response times in the ps domain. Inherently better time resolution is provided by mode locked and synchronously pumped dye laser pulses for the excitation of nucleoside fluorescence. Their detection however has been limited by the time response of photodetectors usually in the range of a few hundred ps. Here we demonstrate the advantage of using multichannel plate detectors with short transient time jitter for single photon detection (4) together with deconvolution procedures for measurements of decay times of a few ps as well as for time resolved fluorescence spectroscopy of nucleic acids.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Daniels,M.: in “Physico-chemical properties of nucleic acids” (I.Duchesne, ed.), Academic Press, London-New York (1973), p. 99.
Ballini,J.P.,Daniels,M.,Vigny,P.: J. Luminescence 27, 389 (1982).
Ballini,J.P.,Vigny,P.,Daniels,M.: Biophys. Chem. 19, 61 (1983).
Oba,K.,Ito,M.: Computer analysis of timing properties of micro channel plate photomultiplier tube. Proceed. 8th Symposium on Photoelectronic image devices, Imperial College of Science and Technology, London, Sept. 1983.
Rigler,R.,Ehrenberg,M.: Quart. Rev. Biophys. 9, 1 (1976).
Beddard,G.S.,Doust,T.,Porter,G.: Chem. Phys. 61, 17 (1981).
Ehrenberg,M.,Cronvall,E.,Rigler,R.: Febs Letters 18, 199 (1971).
Mahr,H.,Hirsch,M.D.: Opt. Commun. 13, 96 (1975).
Karplus,M.,McCammon,J.A.: Ann. Rev. Biochem. 53, 263 (1983).
Börresen,H.C.: Acta Chem. Scand. 17, 921 ( 1969.
Morgan,J.P.,Daniels,M.: PhotochemnPhotobiol. 31, 101 (1979).
Hélène,C.,Michelson,A.M.: BBA 142, 12 (1966).
Eisinger,J.,Guéron,M.,Shulman,R.G.,Yamane,T.: Proc. Natl. Acad. Sci. 55, 1015 (1966).
Eisinger,J.,Shulman,R.G.: J. Mol. Biol. 28, 445 (1967).
Hélène,C.: in “Physico-chemical properties of nucleic acids (I.Duchesne, ed.), Academic Press, London-New York (1973), p. 119.
Shapiro,S.L.: in “Biological Events Probed by Ultrafast Laser Spectroscopy” (R.R.Alfano, ed.), Academic Press, New York-London (1982), p. 161.
Ehrenberg,M.,Rigler,R.: Chem. Phys. Letters 14, 539 (1972).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Rigler, R., Claesens, F., Lomakka, G. (1984). Picosecond Single Photon Fluorescence Spectroscopy of Nucleic Acids. In: Auston, D.H., Eisenthal, K.B. (eds) Ultrafast Phenomena IV. Springer Series in Chemical Physics, vol 38. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-82378-7_126
Download citation
DOI: https://doi.org/10.1007/978-3-642-82378-7_126
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-82380-0
Online ISBN: 978-3-642-82378-7
eBook Packages: Springer Book Archive