Abstract
This chapter will be primarily concerned with techniques that have been devised for isolating and studying the lampbrush chromosomes of Amphibia. No attempt will be made to cover conventional methods applied to whole oocytes for light or electron microscopy, though it is worth a mention that in my experience Sanfelice’s (1918) fixative is by far the most reliable for morphological studies with the light microscope on fixed, sectioned and stained oocyte nuclei. Two accounts of the procedures appropriate for isolating and examining lampbrush chromosomes are available in the earlier literature (Callan and Lloyd 1960, Gall 1966) but they do not include recent technical developments; several of these are to be found in Macgregor and Varley’s (1983) book on techniques for studying the chromosomes of animals.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 1986 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Callan, H.G. (1986). Techniques for the Study of Lampbrush Chromosomes. In: Lampbrush Chromosomes. Molecular Biology, Biochemistry and Biophysics, vol 36. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-82792-1_2
Download citation
DOI: https://doi.org/10.1007/978-3-642-82792-1_2
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-82794-5
Online ISBN: 978-3-642-82792-1
eBook Packages: Springer Book Archive