Skip to main content

Aconitum spp. (Monkshood): Somatic Embryogenesis, Plant Regeneration, and the Production of Aconitine and Other Alkaloids

  • Chapter
Medicinal and Aromatic Plants III

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 15))

Abstract

Aconitum spp., aconite or monkshood, is a perennial herb of the family Ranun-culaceae. It is erect and grows to a height of about 1 m. The leaves are deep green and lustrous, and purple flowers bloom in the autumn. The aconite consists of both parent and daughter roots. Both are obconical in shape, dark brown in color, from 4 to 10 cm in length and from 1 to 3 cm in diameter at the crown. In northern countries, natives used the root extract of the plants as an arrow poison. In Japan this poison was also used by the Ainu for making poisoned arrows for bear hunting. It is well known that the roots contain diterpenoid alkaloids which are mainly classified into a strongly toxic group(aconitine-type) and a weakly toxic group(atisine type). The former include aconitine, mesaconitine, hypaconitine, jesaconitine, and neopelline. The latter covers anisine, kobushine, pseudokobusine, telatisine, songorine, atidine, napelline, heteratisine, ignarine, and hysognavine. The roots also contain a cardiac-activating alkaloid, higenamine (Kosuge and Yokota 1976), and coryneine (Konno et al. 1984) (Fig. 1).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Cervelli R (1986) Somatic embryogenesis and shoot multiplication of the threatened perennial: northern monkshood Aconitum noveboracens. In: 6th Int Congr Plant tissue and cell cultures, Minneapolis, MN,Abstr, p 189.

    Google Scholar 

  • Cervelli R (1987) In vitro propagation of Aconitum noveboracense and Aconitum napellus. HortSci 22:304–305.

    CAS  Google Scholar 

  • Chang W, Hsing Y (1980) In vitro flowering of embryoid derived from mature root culture of ginseng (Panax ginseng). Nature 284:340–342.

    Article  Google Scholar 

  • Hatano K, Shoyama Y, Nishioka I (1984) Study on Aconitum spp. (2) Propagation of Aconitum carmichaeli by anther culture. In: 31st Annu Meet Jpn Soc Pharmacognosy, Abstr, p 69.

    Google Scholar 

  • Hatano K, Shoyama Y, Nishioka I (1985) Study on Aconitum spp. (3) Clonal propagation of Aconitum carmichaeli by tip tissue culture. In: 105th Annu Meet Pharmaceut Soc Jpn, Abstr, p 429.

    Google Scholar 

  • Hatano K, Shoyama Y, Nishioka I (1987) Somatic embryogenesis and plant regeneration from the anther of Aconitum carmichaeli Debx. Plant Cell Rep 6:446–448.

    CAS  Google Scholar 

  • Hatano K, Kamura K, Shoyama Y. Nishoka I (1988) Clonal multiplication of Aconitum carmichaeli by tip tissue culture and alkaloid contents of clonally propagated plant. Planta Med 54:152–155.

    Article  PubMed  CAS  Google Scholar 

  • Hikino H, Konno C (1981) Determination of aconitine alkaloids by high-performance liquid chromatography. J Chromatogr 211:123–128.

    Article  CAS  Google Scholar 

  • Hikino H, Murakami M, Konno C, Watanabe H (1983) Determination of aconitine alkaloids in Aconitum root. Planta Med 48:67–71.

    Article  PubMed  CAS  Google Scholar 

  • Hiraoka N, Kodama T, Oyanagi M, Nakano S, Tomita Y, Yamada N, Iida O, Satake M (1986) Characteristics of Bupleurum falcatum plants propagated through somatic embryogenesis of callus culture. Plant Cell Rep 5:319–321.

    Article  CAS  Google Scholar 

  • Kitagawa I, Yoshikawa M, Chen ZL, Kobayashi K (1982) Four new lipo-alkaloids from aconiti tuber. Chem Pharm Bull 30:758–761.

    Article  CAS  Google Scholar 

  • Kitagawa I, Chen ZL, Yoshihara M, Yoshikawa M (1984a) Chemical studies on crude drug processing II. Aconiti tuber (1). On the constituents of “Chuon-wu”, the dried tuber of Aconitum carmichaeli Debx. Yakugaku Zasshi 104:848–857.

    PubMed  CAS  Google Scholar 

  • Kitagawa I, Chen ZL, Yoshihara M, Yoshikawa M (1984b) Chemical studies on crude drug processing IV. Aconiti tuber (3). Quantitative determination of aconitine alkaloids in aconiti tuber by means of high performance liquid chromatography. Yakugaku Zasshi 104:867–872.

    PubMed  CAS  Google Scholar 

  • Konno C, Shirasaka M, Hikino H (1984) Cardioactive principle of Aconitum carmichaeli roots. Planta Med 35:150–155.

    Article  Google Scholar 

  • Kosuge T, Yokota M (1976) Studies on cardiac principle of aconiti root. Chem Pharm Bull 24:176–178.

    Article  PubMed  CAS  Google Scholar 

  • Miura Y, Fukui H, Tabata M (1988) Reduced inhomogeneity of Angelica acutiloba plants propagated clonally through somatic embryoids. Planta Med 54:79–81.

    Article  PubMed  CAS  Google Scholar 

  • Mori T, Ohsawa T, Murayama M, Bando H, Wada K, Amiya T (1989) Studies on Aconitum species. VIII. Components of “Kako-bushimatsu”. Heterocycles 29:873–885.

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497.

    Article  CAS  Google Scholar 

  • Sakai K (1933) A brief note on the chromosomes in some Aconitum species. Trans Sapporo Nat Hist Soc 13:74–77.

    Google Scholar 

  • Shono K (1965) Changes in organ forming capacity of carrot root calluses during subcultures. Plant Cell Physiol 6:403.

    Google Scholar 

  • Shoyama Y, Nishioka I, Fujioka N, Kohda H, Yamasaki K (1987) Clonal multiplication of Panax japonicus by tissue culture. Shoyakugaku Zasshi 41:333–337.

    Google Scholar 

  • Shoyama Y, Kamura K, Nishioka I (1988) Somatic embryogenesis and clonal multiplication of Panax ginseng. Planta Med 54:155–156.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1991 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Shoyama, Y., Nishioka, I., Hatano, K. (1991). Aconitum spp. (Monkshood): Somatic Embryogenesis, Plant Regeneration, and the Production of Aconitine and Other Alkaloids. In: Bajaj, Y.P.S. (eds) Medicinal and Aromatic Plants III. Biotechnology in Agriculture and Forestry, vol 15. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84071-5_4

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-84071-5_4

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-84073-9

  • Online ISBN: 978-3-642-84071-5

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics